Viscoelastic Oxidized Alginates with Reversible Imine Type Crosslinks: Self-Healing, Injectable, and Bioprintable Hydrogels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Alginate Oxidation and Model Reactions
2.2. Hydrogel Formation
2.3. Rheology
2.3.1. Storage and Loss Moduli
2.3.2. Viscoelasticity
2.3.3. Self-Healing
2.3.4. Cell Viability
2.3.5. Cell Spreading
2.4. Injectability and Bioprintability
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Alginate Purification
4.3. Synthesis of Oxidized Alginate
4.4. Preparation of Hydrogels for Rheology and Cell Culture
4.5. Rheological Measurements
4.6. Macroscopic Self-Healing Experiment
4.7. Printability and Injectability
4.8. Cell Culture
4.9. Viability Assays
4.10. Bioprintability Viability
4.11. Cell Spreading
4.12. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Hafeez, S.; Ooi, H.W.; Morgan, F.L.C.; Mota, C.; Dettin, M.; Van Blitterswijk, C.; Moroni, L.; Baker, M.B. Viscoelastic Oxidized Alginates with Reversible Imine Type Crosslinks: Self-Healing, Injectable, and Bioprintable Hydrogels. Gels 2018, 4, 85. https://doi.org/10.3390/gels4040085
Hafeez S, Ooi HW, Morgan FLC, Mota C, Dettin M, Van Blitterswijk C, Moroni L, Baker MB. Viscoelastic Oxidized Alginates with Reversible Imine Type Crosslinks: Self-Healing, Injectable, and Bioprintable Hydrogels. Gels. 2018; 4(4):85. https://doi.org/10.3390/gels4040085
Chicago/Turabian StyleHafeez, Shahzad, Huey Wen Ooi, Francis L. C. Morgan, Carlos Mota, Monica Dettin, Clemens Van Blitterswijk, Lorenzo Moroni, and Matthew B. Baker. 2018. "Viscoelastic Oxidized Alginates with Reversible Imine Type Crosslinks: Self-Healing, Injectable, and Bioprintable Hydrogels" Gels 4, no. 4: 85. https://doi.org/10.3390/gels4040085