On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery
Abstract
:1. Introduction
2. Results and Discussion
- a)
- functionalization of the magnetite (Fe3O4) NPs with APTMS in order to introduce -NH2 groups on the NPs surface (NPs-NH2); and
- b)
- binding of CMC polymer to the (Fe3O4) NPs-NH2, via the formation of an amide bond between the CMC carboxylic groups and the -NH2 groups on the NPs surface in order to get the hybrid hydrogel.
2.1. Characterization and Morphological Analysis of the NPs-NH2 and the Hydrogel CMC-NPs
Fe3O4 NPs | Fe3O4 NPs-NH2 | |
---|---|---|
Size (nm) | 2004 ± 207 | 499 ± 10 |
PDI ζ-potential | 0.88 ± 0.09 17 ± 2 | 0.35 ± 0.08 23 ± 1 |
2.2. Water Uptake (WU) and Rheological Measurements
Sample | G' (Pa) | G" (Pa) |
---|---|---|
CMC-DAP | 1025 ± 300 | 300 ± 90 |
CMC-NPs (Fe3O4) | 3250 ± 120 | 258 ± 30 |
2.3. Magnetic Properties
Sample | 0Hc 2.5 K (mT) a | MS 2.5 K (Am2/kg) b | MR 2.5 K c | 0Hc 300 K (mT) d | MS 300 K (Am2/kg) e | MR 300 K f |
---|---|---|---|---|---|---|
NPs | 24.6 | 86 | 0.39 | 5.0 | 76 | 0.17 |
NPs-NH2 | 23.2 | 83 | 0.37 | 4.6 | 74 | 0.13 |
CMC-NPs hydrogel | 27.8 | 16 | 0.27 | 6.8 | 14 | 0.12 |
2.4. Biological Tests
2.5. Release of Doxorubicin from Drug-Loaded CMC-NPs Hydrogel
3. Conclusions
4. Materials and methods
4.1. Materials
4.2. Methods
4.2.1. Functionalization of Fe3O4 NPs
4.2.2. Synthesis of the Hybrid Hydrogel
4.2.3. FT-IR and UV-Visible Spectroscopy
4.2.4. Dynamic Light Scattering (DLS)
4.2.5. FESEM and STEM
4.2.6. Water Uptake
4.2.7. Rheological Measurements
4.2.8. Magnetic Properties
4.2.9. Biological Tests
4.2.10. Drug Loading and Release
Acknowledgements
Conflicts of Interest
References
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Uva, M.; Mencuccini, L.; Atrei, A.; Innocenti, C.; Fantechi, E.; Sangregorio, C.; Maglio, M.; Fini, M.; Barbucci, R. On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery. Gels 2015, 1, 24-43. https://doi.org/10.3390/gels1010024
Uva M, Mencuccini L, Atrei A, Innocenti C, Fantechi E, Sangregorio C, Maglio M, Fini M, Barbucci R. On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery. Gels. 2015; 1(1):24-43. https://doi.org/10.3390/gels1010024
Chicago/Turabian StyleUva, Marianna, Lorenzo Mencuccini, Andrea Atrei, Claudia Innocenti, Elvira Fantechi, Claudio Sangregorio, Melania Maglio, Milena Fini, and Rolando Barbucci. 2015. "On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery" Gels 1, no. 1: 24-43. https://doi.org/10.3390/gels1010024