H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Media, and Growth Conditions
2.2. Gene Deletion and Organelle Labelling
2.3. Confocal and Airyscan Microscopy
3. Results
3.1. H2O2 and CCCP Induce Mitochondrial Constriction and Membrane Depolarization
3.2. H2O2-Induced Mitochondrial Constriction Sites Are in Contact with the Endoplasmic Reticulum
3.3. Mitochondrial Constrictions and ER-Dotted Structures Are Formed during Asexual Development
3.4. Calcium Is Required for Mitochondrial Constrictions Induced by H2O2 or CCCP but Not for the Remodeling of the Mitochondrial Outer Membrane
3.5. MdmB, a Subunit of the ERMES Complex, Is Required for Mitochondrial Constriction and Division
4. Discussion
4.1. H2O2 Induces Mitochondrial Depolarization, Constriction and Rearrangements of the Outer Mitochondrial Membrane
4.2. Intracellular Ca2+ and MdmB, a Subunit of the ERMES Complex, Are Required for H2O2-Induced Mitochondrial Constriction and Division
4.3. H2O2 Differential Roles in Mitochondrial Dynamics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Garrido-Bazán, V.; Aguirre, J. H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans. J. Fungi 2022, 8, 829. https://doi.org/10.3390/jof8080829
Garrido-Bazán V, Aguirre J. H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans. Journal of Fungi. 2022; 8(8):829. https://doi.org/10.3390/jof8080829
Chicago/Turabian StyleGarrido-Bazán, Verónica, and Jesús Aguirre. 2022. "H2O2 Induces Calcium and ERMES Complex-Dependent Mitochondrial Constriction and Division as Well as Mitochondrial Outer Membrane Remodeling in Aspergillus nidulans" Journal of Fungi 8, no. 8: 829. https://doi.org/10.3390/jof8080829