Comparative Copper Resistance Strategies of Rhodonia placenta and Phanerochaete chrysosporium in a Copper/Azole-Treated Wood Microcosm
Abstract
:1. Introduction
2. Material and Methods
2.1. Microcosm Set-Up
2.2. Respiration Tests
2.3. X-ray Fluorescence Spectroscopy and Inductively Coupled Plasma Atomic Emission Spectrometry for Copper Quantification in the Liquid Phase
2.4. Scanning Electron Microscopy (SEM) and Electron Dispersive Spectroscopy (EDS) Microanalyses for Copper Quantification in Sawdust and Fungal Hyphae
2.5. High Performance Liquid Chromatography for Soluble Oxalate Quantification
2.6. Statistical Analyses
2.7. Genome Mining to Compare Copper Related Genes in R. placenta and P. chrysosporium
3. Results
3.1. Efficient Growth of Both the Brown-Rot Rhodonia Placenta and the White-Rot Phanerochaete Chrysosporium on Tanalith E3474-Treated Wood
3.2. Comparative Copper Bioleaching Ability of R. placenta and P. chrysosporium from Tanalith E3474 Treated Wood
3.3. SEM-EDS Based Comparative Analysis of Copper Detoxification Strategies between R. placenta and P. chrysosporium
3.4. Comparative Genomics of Copper-Related Genes in R. placenta and P. chrysosporium
4. Discussion
5. Conclusions and Perspectives
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pandharikar, G.; Claudien, K.; Rose, C.; Billet, D.; Pollier, B.; Deveau, A.; Besserer, A.; Morel-Rouhier, M. Comparative Copper Resistance Strategies of Rhodonia placenta and Phanerochaete chrysosporium in a Copper/Azole-Treated Wood Microcosm. J. Fungi 2022, 8, 706. https://doi.org/10.3390/jof8070706
Pandharikar G, Claudien K, Rose C, Billet D, Pollier B, Deveau A, Besserer A, Morel-Rouhier M. Comparative Copper Resistance Strategies of Rhodonia placenta and Phanerochaete chrysosporium in a Copper/Azole-Treated Wood Microcosm. Journal of Fungi. 2022; 8(7):706. https://doi.org/10.3390/jof8070706
Chicago/Turabian StylePandharikar, Gaurav, Kévin Claudien, Christophe Rose, David Billet, Benoit Pollier, Aurélie Deveau, Arnaud Besserer, and Mélanie Morel-Rouhier. 2022. "Comparative Copper Resistance Strategies of Rhodonia placenta and Phanerochaete chrysosporium in a Copper/Azole-Treated Wood Microcosm" Journal of Fungi 8, no. 7: 706. https://doi.org/10.3390/jof8070706