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Intake of Koji Amazake Improves Defecation Frequency in Healthy Adults

Hakkaisan Brewery Co., Ltd., 1051 Nagamori, Minamiuonuma City, Niigata 949-7112, Japan
Department of Endocrinology and Metabolism, Niigata University Graduate School of Medical and Dental Sciences, 754 Ichibancho, Asahimachi-dori, Chuo-ku, Niigata City, Niigata 951-8510, Japan
Niigata Medical Association of Occupational Health Inc., 1185-3 Kitaba, Nishi-ku, Niigata City, Niigata 950-1187, Japan
Niigata Bio-Research Park Inc., 316-2 Higashijima, Akiha-ku, Niigata City, Niigata 956-0841, Japan
Author to whom correspondence should be addressed.
Academic Editors: Katsuhiko Kitamoto and Yujiro Higuchi
J. Fungi 2021, 7(9), 782;
Received: 1 September 2021 / Revised: 17 September 2021 / Accepted: 18 September 2021 / Published: 21 September 2021
(This article belongs to the Special Issue Aspergillus oryzae and related Koji molds)
Reportedly, the intake of koji amazake, a beverage made from steamed rice fermented by Aspergillus oryzae, improves defecation frequency. However, its functional ingredients and mechanism of action remain unclear. To compare the effects of koji amazake and a placebo beverage on defecation frequency and to identify the functional ingredients and mechanism of action, a randomized, placebo-controlled, double-blind parallel-group comparative trial was performed on two groups. The koji amazake had 302 ± 15.5 mg/118 g of A. oryzae cells, which was not in the placebo. Compared with the placebo group, the koji amazake group showed a significant increase in weekly defecation frequency at 2 weeks (5.09 days vs. 4.14 days), 3 weeks (5.41 days vs. 4.18 days), and 4 weeks (5.09 days vs. 3.95 days), along with an increase in the weekly fecal weight at 4 weeks (724 g vs. 501 g). The intake of koji amazake did not induce significant intergroup differences in the fecal SCFA concentration, whereas it significantly decreased the relative abundance of Blautia and significantly increased that of Bacteroides at 3 weeks. Therefore, koji amazake intake improved defecation frequency, and A. oryzae cells played potentially important roles as functional ingredients. View Full-Text
Keywords: amazake; Aspergillus oryzae; defecation; intestinal microbiota; koji amazake; Aspergillus oryzae; defecation; intestinal microbiota; koji
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MDPI and ACS Style

Kurahashi, A.; Enomoto, T.; Oguro, Y.; Kojima-Nakamura, A.; Kodaira, K.; Watanabe, K.; Ozaki, N.; Goto, H.; Hirayama, M. Intake of Koji Amazake Improves Defecation Frequency in Healthy Adults. J. Fungi 2021, 7, 782.

AMA Style

Kurahashi A, Enomoto T, Oguro Y, Kojima-Nakamura A, Kodaira K, Watanabe K, Ozaki N, Goto H, Hirayama M. Intake of Koji Amazake Improves Defecation Frequency in Healthy Adults. Journal of Fungi. 2021; 7(9):782.

Chicago/Turabian Style

Kurahashi, Atsushi, Toshihiko Enomoto, Yoshifumi Oguro, Ayana Kojima-Nakamura, Kazuya Kodaira, Kenichi Watanabe, Nobuhiro Ozaki, Hiroshi Goto, and Masao Hirayama. 2021. "Intake of Koji Amazake Improves Defecation Frequency in Healthy Adults" Journal of Fungi 7, no. 9: 782.

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