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Article

mRNA Inventory of Extracellular Vesicles from Ustilago maydis

1
Institute for Microbiology, Cluster of Excellence on Plant Sciences, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
2
Bioinformatics and Systems Biology, Justus-Liebig-Universität, 35392 Giessen, Germany
3
Biocenter of the LMU Munich, Genetics Section, Grosshaderner Str. 2-4, 82152 Planegg-Martinsried, Germany
4
Institute for Mathematical Modelling of Biological Systems, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Kuang R. Chung
J. Fungi 2021, 7(7), 562; https://doi.org/10.3390/jof7070562
Received: 8 June 2021 / Revised: 2 July 2021 / Accepted: 7 July 2021 / Published: 14 July 2021
(This article belongs to the Special Issue Signal Transductions in Fungi)
Extracellular vesicles (EVs) can transfer diverse RNA cargo for intercellular communication. EV-associated RNAs have been found in diverse fungi and were proposed to be relevant for pathogenesis in animal hosts. In plant-pathogen interactions, small RNAs are exchanged in a cross-kingdom RNAi warfare and EVs were considered to be a delivery mechanism. To extend the search for EV-associated molecules involved in plant-pathogen communication, we have characterised the repertoire of EV-associated mRNAs secreted by the maize smut pathogen, Ustilago maydis. For this initial survey, we examined EV-enriched fractions from axenic filamentous cultures that mimic infectious hyphae. EV-associated RNAs were resistant to degradation by RNases and the presence of intact mRNAs was evident. The set of mRNAs enriched inside EVs relative to the fungal cells are functionally distinct from those that are depleted from EVs. mRNAs encoding metabolic enzymes are particularly enriched. Intriguingly, mRNAs of some known effectors and other proteins linked to virulence were also found in EVs. Furthermore, several mRNAs enriched in EVs are also upregulated during infection, suggesting that EV-associated mRNAs may participate in plant-pathogen interactions. View Full-Text
Keywords: extracellular vesicles (EVs); mRNA; fungal pathogen; plant pathogen; Ustilago maydis extracellular vesicles (EVs); mRNA; fungal pathogen; plant pathogen; Ustilago maydis
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MDPI and ACS Style

Kwon, S.; Rupp, O.; Brachmann, A.; Blum, C.F.; Kraege, A.; Goesmann, A.; Feldbrügge, M. mRNA Inventory of Extracellular Vesicles from Ustilago maydis. J. Fungi 2021, 7, 562. https://doi.org/10.3390/jof7070562

AMA Style

Kwon S, Rupp O, Brachmann A, Blum CF, Kraege A, Goesmann A, Feldbrügge M. mRNA Inventory of Extracellular Vesicles from Ustilago maydis. Journal of Fungi. 2021; 7(7):562. https://doi.org/10.3390/jof7070562

Chicago/Turabian Style

Kwon, Seomun, Oliver Rupp, Andreas Brachmann, Christopher F. Blum, Anton Kraege, Alexander Goesmann, and Michael Feldbrügge. 2021. "mRNA Inventory of Extracellular Vesicles from Ustilago maydis" Journal of Fungi 7, no. 7: 562. https://doi.org/10.3390/jof7070562

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