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Article

Global Transcriptome Profiling Identified Transcription Factors, Biological Process, and Associated Pathways for Pre-Harvest Aflatoxin Contamination in Groundnut

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Center of Excellence in Genomics & Systems Biology (CEGSB), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India
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Department of Genetics, Osmania University, Hyderabad 500007, India
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College of Life Science, China Jiliang University (CJLU), Hangzhou 310018, China
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Department of Biotechnology, University of Agricultural Sciences, Dharwad 580005, India
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Theme-Integrated Crop Improvement, Research Program-Asia, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India
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Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Starkville, MS 39762, USA
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Crop Genetics and Breeding Research Unit, USDA-ARS, Tifton, GA 31793, USA
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State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University, Murdoch, WA 6150, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Joseph M. Bliss
J. Fungi 2021, 7(6), 413; https://doi.org/10.3390/jof7060413
Received: 1 May 2021 / Revised: 20 May 2021 / Accepted: 23 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Host Defense against Fungi)
Pre-harvest aflatoxin contamination (PAC) in groundnut is a serious quality concern globally, and drought stress before harvest further exacerbate its intensity, leading to the deterioration of produce quality. Understanding the host–pathogen interaction and identifying the candidate genes responsible for resistance to PAC will provide insights into the defense mechanism of the groundnut. In this context, about 971.63 million reads have been generated from 16 RNA samples under controlled and Aspergillus flavus infected conditions, from one susceptible and seven resistant genotypes. The RNA-seq analysis identified 45,336 genome-wide transcripts under control and infected conditions. This study identified 57 transcription factor (TF) families with major contributions from 6570 genes coding for bHLH (719), MYB-related (479), NAC (437), FAR1 family protein (320), and a few other families. In the host (groundnut), defense-related genes such as senescence-associated proteins, resveratrol synthase, seed linoleate, pathogenesis-related proteins, peroxidases, glutathione-S-transferases, chalcone synthase, ABA-responsive gene, and chitinases were found to be differentially expressed among resistant genotypes as compared to susceptible genotypes. This study also indicated the vital role of ABA-responsive ABR17, which co-regulates the genes of ABA responsive elements during drought stress, while providing resistance against A. flavus infection. It belongs to the PR-10 class and is also present in several plant–pathogen interactions. View Full-Text
Keywords: aflatoxin; Aspergillus flavus; biotechnology; food safety; gene expression; genomics; pre-harvest aflatoxin contamination (PAC); RNA-seq; transcriptome analysis aflatoxin; Aspergillus flavus; biotechnology; food safety; gene expression; genomics; pre-harvest aflatoxin contamination (PAC); RNA-seq; transcriptome analysis
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MDPI and ACS Style

Soni, P.; Pandey, A.K.; Nayak, S.N.; Pandey, M.K.; Tolani, P.; Pandey, S.; Sudini, H.K.; Bajaj, P.; Fountain, J.C.; Singam, P.; Guo, B.; Varshney, R.K. Global Transcriptome Profiling Identified Transcription Factors, Biological Process, and Associated Pathways for Pre-Harvest Aflatoxin Contamination in Groundnut. J. Fungi 2021, 7, 413. https://doi.org/10.3390/jof7060413

AMA Style

Soni P, Pandey AK, Nayak SN, Pandey MK, Tolani P, Pandey S, Sudini HK, Bajaj P, Fountain JC, Singam P, Guo B, Varshney RK. Global Transcriptome Profiling Identified Transcription Factors, Biological Process, and Associated Pathways for Pre-Harvest Aflatoxin Contamination in Groundnut. Journal of Fungi. 2021; 7(6):413. https://doi.org/10.3390/jof7060413

Chicago/Turabian Style

Soni, Pooja, Arun K. Pandey, Spurthi N. Nayak, Manish K. Pandey, Priya Tolani, Sarita Pandey, Hari K. Sudini, Prasad Bajaj, Jake C. Fountain, Prashant Singam, Baozhu Guo, and Rajeev K. Varshney 2021. "Global Transcriptome Profiling Identified Transcription Factors, Biological Process, and Associated Pathways for Pre-Harvest Aflatoxin Contamination in Groundnut" Journal of Fungi 7, no. 6: 413. https://doi.org/10.3390/jof7060413

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