Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Strain and Culture Conditions
2.2. Growth Rate Determination
2.3. Identification and Quantification of Compatible Solutes
2.4. Compatible Solutes in Salt-Adapted Mycelium
2.5. Compatible Solutes after Osmotic Shock
2.6. RNA Extraction and qPCR Analysis
2.7. Phylogenetic Analysis of MAPK Orthologs
2.8. Western Blot of Phosphorylated Hog
2.9. Na+/K+ Quantification
2.10. Quantification of Oxidative Stress Markers and Antioxidant Responses in A. sydowii
2.11. Statistical Analysis
3. Results and Discussion
3.1. Compatible Solutes in Salt-Adapted A. sydowii Cells
3.2. Transcriptional Profiling of Genes Coding for Osmolyte Synthesis Enzymes
3.3. Dynamics of Compatible Solutes Accumulation after Hypoosmotic or Hyperosmotic Shock
3.4. Hog Phosphorylation Response to Osmotic Shock
3.5. Na+/K+ Ratio in A. sydowii
3.6. Stress Induces Changes in the Redox State of A. sydowii
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Rodríguez-Pupo, E.C.; Pérez-Llano, Y.; Tinoco-Valencia, J.R.; Sánchez, N.S.; Padilla-Garfias, F.; Calahorra, M.; Sánchez, N.d.C.; Sánchez-Reyes, A.; Rodríguez-Hernández, M.d.R.; Peña, A.; et al. Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock. J. Fungi 2021, 7, 414. https://doi.org/10.3390/jof7060414
Rodríguez-Pupo EC, Pérez-Llano Y, Tinoco-Valencia JR, Sánchez NS, Padilla-Garfias F, Calahorra M, Sánchez NdC, Sánchez-Reyes A, Rodríguez-Hernández MdR, Peña A, et al. Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock. Journal of Fungi. 2021; 7(6):414. https://doi.org/10.3390/jof7060414
Chicago/Turabian StyleRodríguez-Pupo, Eya Caridad, Yordanis Pérez-Llano, José Raunel Tinoco-Valencia, Norma Silvia Sánchez, Francisco Padilla-Garfias, Martha Calahorra, Nilda del C. Sánchez, Ayixón Sánchez-Reyes, María del Rocío Rodríguez-Hernández, Antonio Peña, and et al. 2021. "Osmolyte Signatures for the Protection of Aspergillus sydowii Cells under Halophilic Conditions and Osmotic Shock" Journal of Fungi 7, no. 6: 414. https://doi.org/10.3390/jof7060414