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Article

Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM

1
Department of Laboratory Medicine, AZ Delta Hospital, Deltalaan 1, 8800 Roeselare, Belgium
2
Department of Microbiology and Infection Control, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Laarbeeklaan 101, 1090 Brussels, Belgium
3
Department of Laboratory Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium
4
Department of Laboratory Medicine and National Reference Centre for Mycosis, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium
*
Author to whom correspondence should be addressed.
J. Fungi 2021, 7(5), 356; https://doi.org/10.3390/jof7050356
Received: 28 March 2021 / Revised: 26 April 2021 / Accepted: 28 April 2021 / Published: 1 May 2021
Two colorimetric broth microdilution antifungal susceptibility tests were compared, Sensititre YeastOne and MICRONAUT-AM for nine antifungal agents. One hundred clinical Candida isolates were tested, representing a realistic population for susceptibility testing in daily practice. The reproducibility characteristics were comparable. Only for fluconazole, caspofungin, 5-flucytosine and amphotericin B, an essential agreement of ≥90% could be demonstrated. Sensititre minimal inhibitory concentrations (MICs) were systematically higher than MICRONAUT MICs for all antifungals, except for itraconazole. CLSI clinical breakpoints (CBPs) and epidemiological cut-off values (ECVs) were used for Sensititre MICs while for MICRONAUT the EUCAST CBPs and ECVs were used. Only fluconazole, micafungin, and amphotericin B had a categorical agreement of ≥90%. For fluconazole, micafungin, and amphotericin B the susceptibility proportions were comparable. Susceptibility proportion of posaconazole and voriconazole was higher using the MICRONAUT system. For itraconazole and anidulafungin, the susceptibility proportion was higher using Sensititre. It was not possible to determine the true MIC values or the correctness of a S/I/R result since both commercial systems were validated against a different reference method. These findings show that there is a significant variability in susceptibility pattern and consequently on use of antifungals in daily practice, depending on the choice of commercial system. View Full-Text
Keywords: Candida; antifungal agents; antifungal susceptibility testing; colorimetry; CLSI; EUCAST; MICRONAUT-AM; Sensititre YeastOne; MIC Candida; antifungal agents; antifungal susceptibility testing; colorimetry; CLSI; EUCAST; MICRONAUT-AM; Sensititre YeastOne; MIC
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MDPI and ACS Style

Philips, S.; Van Hoecke, F.; De Laere, E.; Vervaeke, S.; De Smedt, R.; Boelens, J.; De Geyter, D.; Piérard, D.; Lagrou, K. Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM. J. Fungi 2021, 7, 356. https://doi.org/10.3390/jof7050356

AMA Style

Philips S, Van Hoecke F, De Laere E, Vervaeke S, De Smedt R, Boelens J, De Geyter D, Piérard D, Lagrou K. Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM. Journal of Fungi. 2021; 7(5):356. https://doi.org/10.3390/jof7050356

Chicago/Turabian Style

Philips, Sophie, Frederik Van Hoecke, Emmanuel De Laere, Steven Vervaeke, Roos De Smedt, Jerina Boelens, Deborah De Geyter, Denis Piérard, and Katrien Lagrou. 2021. "Comparison of Two Commercial Colorimetric Broth Microdilution Tests for Candida Susceptibility Testing: Sensititre YeastOne versus MICRONAUT-AM" Journal of Fungi 7, no. 5: 356. https://doi.org/10.3390/jof7050356

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