Next Article in Journal
Evaluation of Three Commercial PCR Assays for the Detection of Azole-Resistant Aspergillus fumigatus from Respiratory Samples of Immunocompromised Patients
Previous Article in Journal
Review of T-2307, an Investigational Agent That Causes Collapse of Fungal Mitochondrial Membrane Potential
 
 
Article

Resolution of the Hypoxylon fuscum Complex (Hypoxylaceae, Xylariales) and Discovery and Biological Characterization of Two of Its Prominent Secondary Metabolites

1
Helmholtz-Centre for Infection Research GmbH, Department of Microbial Drugs, Inhoffenstraße 7, 38124 Braunschweig, Germany
2
German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 38124 Braunschweig, Germany
3
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht 4199613776, Iran
4
Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030 Wien, Austria
5
Institute of Forest Entomology, Forest Pathology and Forest Protection, Department of Forest and Soil Sciences, BOKU-University of Natural Resources and Life Sciences, Franz Schwackhöfer Haus, Peter-Jordan-Straße 82/I, 1190 Vienna, Austria
6
Helmholtz-Centre for Infection Research GmbH, Department for Cell Biology, Inhoffenstraße 7, 38124 Braunschweig, Germany
7
Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Lei Cai
J. Fungi 2021, 7(2), 131; https://doi.org/10.3390/jof7020131
Received: 15 December 2020 / Revised: 6 February 2021 / Accepted: 6 February 2021 / Published: 11 February 2021
Hypoxylon, a large, cosmopolitan genus of Ascomycota is in the focus of our current poly-thetic taxonomic studies, and served as an excellent source for bioactive secondary metabolites at the same time. The present work concerns a survey of the Hypoxylon fuscum species complex based on specimens from Iran and Europe by morphological studies and high performance liquid chromatography coupled to mass spectrometry and diode array detection (HPLC-MS-DAD). Apart from known chemotaxonomic markers like binaphthalene tetrol (BNT) and daldinin F, two unprece-dented molecules were detected and subsequently isolated to purity by semi preparative HPLC. Their structures were established by nuclear-magnetic resonance (NMR) spectroscopy as 3′-malonyl-daldinin F (6) and pseudofuscochalasin A (4). The new daldinin derivative 6 showed weak cytotoxicity towards mammalian cells but bactericidal activity. The new cytochalasin 4 was compared to cytochalasin C in an actin disruption assay using fluorescence microscopy of human osteo-sarcoma U2OS cells, revealing comparable activity towards F-actin but being irreversible compared to cytochalasin C. Concurrently, a multilocus molecular phylogeny based on ribosomal and proteinogenic nucleotide sequences of Hypoxylon species resulted in a well-supported clade for H. fuscum and its allies. From a comparison of morphological, chemotaxonomic and phylogenetic evidence, we introduce the new species H. eurasiaticum and H. pseudofuscum. View Full-Text
Keywords: analytical chemistry; Ascomycota; bioactivity screening chemotaxonomy; molecular phylogenetics; polyphasic taxonomy; two new species analytical chemistry; Ascomycota; bioactivity screening chemotaxonomy; molecular phylogenetics; polyphasic taxonomy; two new species
Show Figures

Figure 1

MDPI and ACS Style

Lambert, C.; Pourmoghaddam, M.J.; Cedeño-Sanchez, M.; Surup, F.; Khodaparast, S.A.; Krisai-Greilhuber, I.; Voglmayr, H.; Stradal, T.E.B.; Stadler, M. Resolution of the Hypoxylon fuscum Complex (Hypoxylaceae, Xylariales) and Discovery and Biological Characterization of Two of Its Prominent Secondary Metabolites. J. Fungi 2021, 7, 131. https://doi.org/10.3390/jof7020131

AMA Style

Lambert C, Pourmoghaddam MJ, Cedeño-Sanchez M, Surup F, Khodaparast SA, Krisai-Greilhuber I, Voglmayr H, Stradal TEB, Stadler M. Resolution of the Hypoxylon fuscum Complex (Hypoxylaceae, Xylariales) and Discovery and Biological Characterization of Two of Its Prominent Secondary Metabolites. Journal of Fungi. 2021; 7(2):131. https://doi.org/10.3390/jof7020131

Chicago/Turabian Style

Lambert, Christopher, Mohammad Javad Pourmoghaddam, Marjorie Cedeño-Sanchez, Frank Surup, Seyed Akbar Khodaparast, Irmgard Krisai-Greilhuber, Hermann Voglmayr, Theresia E. B. Stradal, and Marc Stadler. 2021. "Resolution of the Hypoxylon fuscum Complex (Hypoxylaceae, Xylariales) and Discovery and Biological Characterization of Two of Its Prominent Secondary Metabolites" Journal of Fungi 7, no. 2: 131. https://doi.org/10.3390/jof7020131

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop