Multilocus Genotyping and Intergenic Spacer Single Nucleotide Polymorphisms of Amylostereum areolatum (Russulales: Amylostereacea) Symbionts of Native and Non-Native Sirex Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Symbiont Isolation
2.2. Colony Polymerase Chain Reaction (PCR) and Sequencing
2.3. Multilocus Genotyping
2.4. Fragment Analysis of Intergenic Spacer (IGS) Types
2.5. Data Analysis
3. Results
3.1. Phylogenetic Relationship in Amylostereum
3.2. Haplotype Relationships Based on IGS Sequences and Fragment Analysis Data
3.3. Co-Infestation of S. noctilio and S. nitobei in Pinus
4. Discussion
4.1. Multilocus Genotyping and IGS Heterogeneity of A. areolatum
4.2. Sirex noctilio and S. nitobei-A. areolatum Association
4.3. Focus for Further Research
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Composition | Dosage (μL) |
---|---|
The supernatant | 1 |
Premix Taq (Ex Taq Version 2.0) 1.25 U/25 μL | 12.5 |
Primer (F) 10 pmol/μL | 1 |
Primer (R) 10 pmol/μL | 1 |
Sterile distilled water | 9.5 |
Target Gene | Primer Sequence (5′–3′) | Reference | PCR Amplification Conditions |
---|---|---|---|
ITS | 95 °C 4 min; 95 °C 1 min; 55 °C 1 min; 72 °C 1 min; 72 °C 10 min; 35 cycles | ||
ITS1-F | 5′-CTTGGTCATTTAGAGGAAGTAA | Gardes and Bruns (1993) [32] | |
ITS4-B | 5′-CAGGAGACTTGTACACGGTCCAG | ||
tef1 | 95 °C 4 min; 95 °C 1 min; 55 °C 1 min; 72 °C 1 min; 72 °C 10 min; 35 cycles | ||
tef1F | 5′-TACAAGTGCGGTGGTATTGACA | Morehouse et al. (2003) [33] | |
tef1R | 5′-ACGGACTTGACTTCAGTGGT | ||
RPB2 | 95 °C 4 min; 95 °C 1 min; 50 °C 2 min; 72 °C 2 min; 72 °C 10 min; 35 cycles | ||
RPB2-6F | 5′-TGGGGTATGGTCTGTCCTGC | Liu et al. (1999) [34] | |
fRPB2-7.c R | 5′-TGGGGTATGGTCTGTCCTGC | ||
mtSSU | 95 °C 4 min; 95 °C 1 min; 55 °C 1 min; 72 °C 1 min; 72 °C 10 min; 35 cycles | ||
MS1 | 5′-CAGCAGTCAAGAATATTAGTCAATG | White et al. (1990) [35] | |
MS2 | 5′-GCGGATTATCGAATTAAATAAC |
MLG 1 | Isolate Code | ITS-tef1-RPB2-mtSSU Types | Woodwasp/Tree Host | Collection Site | No. Samples | IGS |
---|---|---|---|---|---|---|
A. areolatum | ||||||
A1 | RMK11-001 | A-A-A-A | S. juvencus, S. noctilio, U. albicornis, U. gigas | Denmark, Hungary, and Spain | 16 | BC |
A2 | RMK11-011 | B-A-A-B | S. juvencus | Hungary | 1 | BD |
A3 | RMK11-022 | B-A-B-B | S. noctilio | Hungary | 1 | BD |
A4 | B1395 | C-A-B-B | S. nitobei | Japan | 2 | D2 |
A5 | GR94 | B-A-C-B | S. juvencus, S. noctilio | Australia, Denmark, Hungary, Spain, and US | 14 | BD |
A6 | B1352 | D-A-C-B | tree source (Picea abies) | Germany | 1 | D |
A7 | B1385 | D-A-C-D | S. juvencus | Germany | 1 | BD |
A8 | AH1-17 | B-A-C-E | S. noctilio | US | 1 | D |
A9 | ScyME9/10 | E-B-C-C | S. nitidus | US | 1 | BE |
A10 | Ecogrow | F-C-D-B | Ecogrow nematode culture | Australia | 1 | BD |
A11 | DAOM:239281 | D-C-D-B | S. noctilio | Canada, South Africa, Chile | 8 | BD |
A12 | DAOM:Francke | D-C-D-D | Germany | 1 | BD | |
A13 | D18 | D-D-D-E | S. noctilio, S. nitobei | DM, JBT (this study) | 4 | BD |
A14 | D3 | D-D-E-E | S. noctilio | DM (this study) | 1 | BD |
A15 | D10 | D-E-D-E | S. noctilio | DM, YS, HG, and JBT (this study) | 11 | BD |
A16 | M1 | G-E-B-B | S. nitobei | JBT, LY, YS (this study) | 9 | D |
A17 | L29 | G-D-B-B | S. nitobei | LY (this study) | 1 | D |
A. chailletii | U. gigas | Australia, Denmark, Hungary, Spain, and US | 1 | |||
A. laevigatum | - | U. antennatus | Japan | 1 |
Sirex Host | Samples, n | A. areolatum | A. chailletii | |
---|---|---|---|---|
IGS-D2 | IGS-B1D2 | |||
Sirex noctilio | 26 | - | 26 | - |
Sirex nitobei | 44 | 18 | 2 | 24 |
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Wang, M.; Fu, N.; Gao, C.; Wang, L.; Ren, L.; Luo, Y. Multilocus Genotyping and Intergenic Spacer Single Nucleotide Polymorphisms of Amylostereum areolatum (Russulales: Amylostereacea) Symbionts of Native and Non-Native Sirex Species. J. Fungi 2021, 7, 1065. https://doi.org/10.3390/jof7121065
Wang M, Fu N, Gao C, Wang L, Ren L, Luo Y. Multilocus Genotyping and Intergenic Spacer Single Nucleotide Polymorphisms of Amylostereum areolatum (Russulales: Amylostereacea) Symbionts of Native and Non-Native Sirex Species. Journal of Fungi. 2021; 7(12):1065. https://doi.org/10.3390/jof7121065
Chicago/Turabian StyleWang, Ming, Ningning Fu, Chenglong Gao, Lixia Wang, Lili Ren, and Youqing Luo. 2021. "Multilocus Genotyping and Intergenic Spacer Single Nucleotide Polymorphisms of Amylostereum areolatum (Russulales: Amylostereacea) Symbionts of Native and Non-Native Sirex Species" Journal of Fungi 7, no. 12: 1065. https://doi.org/10.3390/jof7121065