Next Article in Journal
Mixed Etiology COVID-19 Associated Pulmonary Aspergillosis (CAPA)—A Case Report and Brief Review of the Literature
Previous Article in Journal
Regulation of Pkc1 Hyper-Phosphorylation by Genotoxic Stress
 
 
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host

1
Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s/n, Guanajuato 36050, Gto., Mexico
2
Centro de Investigación en Dinámica Celular, Laboratorio de Glicobiología Humana y Diagnóstico Molecular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mor., Mexico
*
Author to whom correspondence should be addressed.
J. Fungi 2021, 7(10), 875; https://doi.org/10.3390/jof7100875
Received: 24 August 2021 / Revised: 8 October 2021 / Accepted: 11 October 2021 / Published: 18 October 2021
(This article belongs to the Section Fungal Pathogenesis and Disease Control)

Abstract

:
Protein glycosylation is a highly conserved post-translational modification among organisms. It plays fundamental roles in many biological processes, ranging from protein trafficking and cell adhesion to host–pathogen interactions. According to the amino acid side chain atoms to which glycans are linked, protein glycosylation can be divided into two major categories: N-glycosylation and O-glycosylation. However, there are other types of modifications such as the addition of GPI to the C-terminal end of the protein. Besides the importance of glycoproteins in biological functions, they are a major component of the fungal cell wall and plasma membrane and contribute to pathogenicity, virulence, and recognition by the host immunity. Given that this structure is absent in host mammalian cells, it stands as an attractive target for developing selective compounds for the treatment of fungal infections. This review focuses on describing the relationship between protein glycosylation and the host–immune interaction in medically relevant fungal species.

1. Introduction

Protein glycosylation is a highly conserved post-translational modification found in both prokaryotic and eukaryotic cells characterized by the addition of oligosaccharides and glycolipids to the peptide backbone. Glycans influence biological processes, such as protein folding, targeted transport, cellular localization, and mediation of host–pathogen interactions, among others [1]. Glycosylation is carried out by different mechanisms that occur in different cell compartments. According to the amide or hydroxyl groups of amino acids to which glycans are attached, glycoproteins are divided into N-linked glycoproteins or O-linked glycoproteins, although other types of glycoproteins have been described that are not part of this work. In the former, glycans are linked to asparagine residues, contained within a consensus sequon, Asn-X-Ser/Thr (where X is any amino acid except for proline) [2]; meanwhile, in the latter, glycans are attached to a subset of serines, threonines, or hydroxylysines [3,4,5].
Another post-translational modification is the anchoring of cell membrane and cell wall proteins to glycosylphosphatidylinositol (GPI) covalently attached to the C-terminus. GPI biosynthesis is a multistep conserved pathway in eukaryotes that culminates in the generation of GPI glycolipids that anchor many proteins to the cell surface. GPI anchor synthesis appears to be essential in lower eukaryotes, affecting growth and viability [6]. Additionally, there is evidence that GPI-anchored proteins are important for the pathogenicity and virulence of many pathogens, as discussed in the following sections.
As mentioned, glycoproteins are involved in a wide range of biological processes which ensure cell stability [7]. For instance, they are a major constituent of the fungal cell wall and plasma membrane, and the biosynthetic pathways of these glycans have been thoroughly dissected in organisms such as Candida albicans and Saccharomyces cerevisiae. Rather than being a rigid and impenetrable shield, the fungal wall is plastic, permeable, and a molecular scaffold to display molecules to respond to changes in the environment or stresses. In addition, it is paramount for viability, morphogenesis, and pathogenesis [8]. This wall is composed of structural polysaccharides (glucans, chitin, and chitosan) and GPI-, N-linked, and O-linked glycoproteins [8]. Moreover, in fungal infections, the cell wall is one of the first points of contact with the host, being essential for fungal pathogenicity and virulence as well as the host’s immune response [9]. The cell wall plays a major role during fungal invasion. It is capable of inducing immune responses due to the components that are not found in the host cells, through the recognition of cell surface fungal-specific molecules, named pathogen-associated molecular patterns, by pattern recognition receptors [10]. Some of the wall antigens are also directly involved in fungal adhesion and colonization of the host tissues [11].
Since N-linked and O-linked glycoproteins, as well as GPI-anchored proteins (GPI-AP), are important secreted proteins, and cell wall and plasma membrane components, it is now well established that they contribute to fungal pathogenicity and virulence, and recognition by the host immunity. In this review, we provide an up-to-date view of these protein modifications in medically relevant fungal species.

2. The Candida albicans Protein Glycosylation Pathways

C. albicans is one of the most common human fungal pathogens that cause benign superficial infections of the mucosa and life-threatening systemic infections [12]. The deep-seated infection is associated with high mortality rates, and treatment is becoming challenging because of the increased number of isolates showing drug resistance to the conventional therapeutic options [12]. Therefore, the C. albicans cell wall biosynthetic routes, including protein glycosylation, and the interaction of the cell wall components with the host immunity have attracted attention in recent decades, with the idea to find targets for the development of new antifungal drugs or immunomodulatory therapies. Since this organism has been regarded as a biological model of a fungal pathogen, its protein glycosylation has been thoroughly studied, and here, we will take these pathways as an example to describe N-linked and O-linked glycosylation, and GPI anchor synthesis.

2.1. The N-Linked Glycosylation Pathway

The first steps of N-linked protein glycosylation are conserved among eukaryotes, such as fungi, plants, protozoa, and mammalian cells, although some peculiarities have been reported in particular species [13,14,15,16]. To obtain the final structure displayed on glycoproteins, N-linked glycans must undergo a series of modifications carried out by glycosidases and glycosyltransferases in the endoplasmic reticulum (ER) and the Golgi complex. This processing can be divided into two sequential stages: (a) the assembly of an oligosaccharide on an isoprenoid lipid in the rough ER by a set of specific glycosyltransferases, and its subsequent translocation to the nascent protein, and (b) further modification of an N-linked glycan by ER glycosidases and Golgi glycosyltransferases [13,17,18]. The enzymes involved in N-linked glycan synthesis are highlighted in Figure 1.

2.1.1. Assembly of Lipid-Linked Oligosaccharide

The lipid moiety of the intermediates involved in protein glycosylation reactions is a polymer of isoprene units (CH3–C(CH3) = CH–CH2–), dolichol (Dol) [2,19,20]. In C. albicans, as in other eukaryotic cells, the synthesis of Dol-PP-GlcNAc2Man5 is achieved in the ER cytoplasmatic face by the addition of N-acetyl-D-glucosamine (GlcNAc) and mannose (Man), using UDP-GlcNAc and GDP-Man as donor substrates [21]. Each of the sugar residues is added to the lipid-linked precursor by a specific glycosyltransferase; Alg7 and Alg13 add phospho-GlcNAc to Dol phosphate, and Alg1, Alg2, Alg11, Alg3, and Alg12 add the first, second, third, fourth, and fifth phospho-Man residues, respectively [22,23]. The precursor is then flipped into the ER lumen in a process that involves the RFT1 product [17]. Within the ER lumen, the mannosyltransferases Alg3, Alg9, and Alg12 add four more mannose residues to the lipid-linked precursor, using Dol-P-Man as the sugar donor, and then Alg6, Alg8, and Alg10 add three glucose (Glc) units to generate the final glycan precursor Dol-PP-GlcNAc2Man9Glc3 [2,13].
Once the precursor is assembled, it is transferred to the nascent protein by the oligosaccharyltransferase enzymatic complex [13,24]. C. albicans encodes all the subunit orthologs found in the S. cerevisiae oligosaccharyltransferase enzymatic complex, which is composed of nine different transmembrane subunits: Wbp1, Swp1, Stt3, Ost1, Ost2, Ost3, Ost4, Ost5, and Ost6, where Stt3 is the catalytic subunit [13,25].

2.1.2. Modification of the N-Linked Glycan Core by Glycosidases and Transferases

Once the oligosaccharide has been transferred to the nascent protein, it is processed by ER α-glycosidases: α-glucosidases I and II, and α-1,2-mannosidase [18,26]. α-Glucosidases I and II remove the terminal α-1,2-linked and two remaining α-1,3-linked glucose residues, respectively, and mannosidase generates the isomer Man8GlcNAc2 [24]. In C. albicans, CWH41 encodes α-glucosidase I, ROT2 and GTB1 encode the heterodimeric α-glucosidase II, and MNS1 encodes the ER mannosidase [3,26].
Following the glycosylation steps in the ER, N-linked glycans are elongated by Golgi mannosyltransferases, which require GDP-Man as a sugar donor, and the transport of this compound into the lumen of the Golgi apparatus is mediated by a specific nucleotide sugar transporter encoded by VRG4 [27]. The α-1,6-mannosyltransferase Och1 starts the addition of an α-1,6-mannose unit to the backbone of the N-linked glycan outer chain (Figure 1) [28]. Subsequently, the α1,6-mannose backbone is extended by the sequential action of the mannan polymerase I (Mnn9 and Van1) and mannan polymerase II (Mnn9, Anp1, Mnn10, Mnn11, and Hoc1) enzyme complexes [13,24,28]. This α-1,6-mannose backbone is branched with α-1,2-oligomannans by the action of mannosyltransferases Mnt3, Mnt4, Mnt5, Mnn2, and Mnn5, and this may be capped with terminal α-1,3-mannoses by the action of the mannosyltransferase Mnn1 (Figure 1) [13]. In C. albicans, it is frequently found that the α-1,2-mannose branches are further β-mannosylated, a mechanism that is carried out by the BMT gene family [29]. Additionally, in this organism, the N-linked glycans can be modified by mannosyl-phosphate moieties, named phosphomannans, which can work as molecular scaffolds to synthesize α-1,2-mannoligosaccharides [24], in a process that involves the MNN4-like gene family and the mannosyltransferases Mnt3 and Mnt5 [30,31].

2.2. The O-Linked Glycosylation Pathway

In C. albicans, the O-linked glycans are linear oligosaccharides of one to seven α-1,2-linked mannose residues [32]. The addition of α-linked mannose residues to the serine or threonine residues is initiated in the ER lumen, using Dol-P-Man as a sugar donor, in a reaction catalyzed by any of the five members of the protein mannosyltransferase (PMT) gene family [13,33]. Then, glycoproteins are transported to the Golgi complex to start the addition of additional mannose residues by the action of Golgi α-1,2-mannosyltransferases encoded by MNT1 and MNT2 [32,34]. These are GDP-mannose-dependent mannosyltransferases that may have redundant activities but with a preference to add particular mannose residues to the O-linked glycans: Mnt1 adds the second mannose, whereas Mnt2 adds the third mannose unit during the elongation step (Figure 1) [32]. These O-linked glycans can also be phosphomannosylated by Mnt3 and Mnt5 [31].

2.3. Glycosylphosphatidylinositol Anchor Synthesis

GPI anchors are structurally complex glycophospholipids that are post-translationally attached to the C-terminal end of proteins containing the appropriate GPI signal sequence [35]. The first step in GPI biosynthesis takes place in the ER, where a phosphatidylinositol receives GlcNAc from UDP–GlcNAc, in a reaction catalyzed by a multi-subunit GPI-N-acetylglucosaminyltransferase (GPI-GnT) complex [6], whose catalytic subunit is encoded by GPI19 (Figure 2) [13,36]. Then, the inositol-acyl group is removed during GPI maturation, triple mannosylated, and decorated with two–three phosphoethanolamine groups at the different mannoses (Figure 2) [6]. In fungi, the addition of a fourth mannose residue is required and catalyzed by the mannosyltransferase Smp3 [37,38]. The complete GPI-anchored protein is formed once the anchor is attached to a new translocated protein in the ER lumen, via the GPI transamidase complex composed of Gaa1, Gpi8, and Gpi16 [39], and is then transported via the secretory pathway to the cell wall or plasma membrane.

3. Protein Glycosylation in Other Medically Relevant Fungal Pathogens

In general terms, the dissected models of C. albicans protein glycosylation can be applied to other fungal species, although species-specific glycans have been isolated from clinically relevant yeasts and molds.
In A. fumigatus, the cell wall contains galactomannan, which is a linear polysaccharide of α-1,2- and α-1,6-mannose units and branched with β-1,5- and β-1,6-galactofuranose residues [40], and is covalently linked to N-linked and O-linked glycans, β-(1,3)-glucans, and cell membrane GPI anchors [40,41]. Galactofuranose-containing glycans are peculiar and not synthesized by the human host. The first step in their synthesis involves the generation of UDP-galactofuranose, by the action of a cytosolic UDP-galactopyranose mutase, encoded by ugm1 [42]; then, a UDP-galactofuranose transporter, GlfB, imports this activated sugar into the Golgi lumen, where the galactofuranosyltransferase GfsA binds this sugar moiety to glycans [43,44,45]. The mannose-rich core of galactomannan is also synthesized in the Golgi complex, and the α-1,2-mannosyltransferases ktr4 and ktr7 have been implicated in this biosynthetic process [46,47]. Since a glfBΔ mutant did not show galactosylated high-mannose N-linked glycans, it was proposed that this transport is required for galactofuranosylation of A. fumigatus N-linked glycans [45]. Even though it is not covalently linked to proteins by the canonical protein glycosylation pathways, it is worthy of mention that galactosaminogalactan, a galactopyranose-containing glycan, is in the surface of A. fumigatus mycelia [48]. In addition to galactopyranose, it also contains galactosamine and N-acetylgalactosamine bound via α-1,4-linkages [48].
The generation of an A. fumigatus undecuple mutant, lacking och1-1, och1-2, och1-3, och1-4, mnn9, van1, anp1, mnn10, mnn11, mnn2, and mnn5, putative orthologs of genes encoding for α-1,2- and α-1,6-mannosyltransferases, did not show a reduction in the hyphal wall mannan content, but this was affected when the conidial cell wall was analyzed [49], suggesting that canonical genes, previously assigned to the protein glycosylation pathways, may have different functions in molds, and that their function is tightly regulated by cell morphology and likely by environmental cues.
Different N-linked and O-linked glycans have also been reported in Cryptococcus spp. There is strong evidence suggesting that the N-linked glycan core is similarly synthesized in both C. albicans and C. neoformans, but the enzymes involved in the synthesis of the N-linked outer chain seem to be absent from the C. neoformans genome [50], suggesting no high-mannose N-linked glycans are synthesized in this organism. However, they may contain sialic acid or xylose [51,52]. Different types of O-linked glycans have been characterized in Cryptococcus laurentii: linear α-1,2-mannotriose and oligomannose chains modified with xylose, and extended chains of α-1,6-galactose linked to a single mannose residue [53]. In the case of C. neoformans, the most abundant O-linked glycans are α-1,2-mannans but connected by an α-1,6-mannose at the third position of the glycan [54]. As a minority, xylose-modified O-linked glycans are also present [54]. Analysis of the biosynthetic machinery behind these structures indicated that the α-1,2-mannosyltransferase Ktr3 is in charge of adding the second mannose residue, and the α-1,6-mannosyltransferases Hoc1 and Hoc3 add the third mannose unit to xylosylated and non-xylosylated O-linked glycans, respectively [54]. Finally, xylose is transferred to glycans from UDP-xylose, which is synthesized by the UXS1 product [54], and XPT1 that codes for a Golgi-resident xylosyltransferase [55].
In Sporothrix schenckii, rhamnose-containing N- and O-linked glycans have been reported and contain terminal α-1,2-, α-1,3-, or α-1,4-rhamnose units [56]. In addition, O-linked glycans mainly contain glucuronic acid, which may be mono- or bi-rhamnosylated [56]. Protein rhamnosylation depends on the presence of RmlD, a gene encoding for an epimerase/reductase essential for the elaboration of UDP-rhamnose, the sugar donor in this post-translational modification [57]. Moreover, sialic acid- and galactose-containing N-linked glycans have been reported in the S. schenckii cell wall [58].

4. Relevance of Fungal Protein Glycosylations during Host–Pathogen Interaction

In pathogenic fungi, many proteins are involved in the N-linked and O-linked glycosylation processes as well in GPI anchor synthesis. Both are important protein modifications in fungi such as C. albicans, A. fumigatus, and C. neoformans, among others, because they intervene in pathogen–host interactions and can directly or indirectly affect virulence [59,60].
The most used strategy to assess the contribution of particular genes to both protein glycosylation and the interaction with the host is the generation of mutant strains and the comparison of their phenotype with the parental strains. In this section, we provide a summary of the contribution of the protein glycosylation pathways to host–fungus interactions (Table 1).

4.1. Relevance of N-Linked Glycosylations during Host–Pathogen Interaction

In C. albicans, the ER α-glucosidase I, α-glucosidase II, and α1,2-mannosidase, encoded by CWH41, ROT2, and MNS1, respectively, are in charge of the N-linked glycan core processing in the ER before transport to the Golgi complex [26]. The C. albicans cwh41Δ and rot2Δ showed cellular aggregates and defects in the cell wall composition, changes that led to virulence attenuation in a murine model of systemic candidiasis, and to a reduced ability to stimulate cytokine production by human peripheral blood mononuclear cells (PBMCs) (Table 1) [26]. In the case of the C. albicans mns1Δ null mutant, fewer phenotypical changes were observed when compared with the mutants with defects in the ER glucosidases, but virulence and stimulated cytokine profiles were still affected (Table 1) [26,63]. These results underscore the relevance of proper N-linked glycan processing for the C. albicans–host interaction. In A. fumigatus, Afcwh41 deletion generated defective processing of N-linked glycans, a reduction in conidia formation, and changes in the cell wall composition, polar growth, and hyphal elongation (Table 1) [61]. However, this gene was not required for virulence [61], contrary to C. albicans. Currently, there are no reports about the function of the homologs of C. albicans ROT2 and MNS1 in A. fumigatus and C. neoformans.
OCH1 disruption, which encodes the α-1,6-mannosyltransferase that begins the synthesis of the N-linked glycan outer chain, has demonstrated to be a valuable tool to understand the importance of fully extended N-linked glycans during the interaction with the host [28,64,65]. The C. albicans och1Δ null mutant showed defects in the cell wall and a decreased growth rate and tended to form aggregates [28]. Importantly, the absence of this gene caused virulence attenuation in a murine model of systemic infection, even though the fungus was able to colonize some organs [28]. Moreover, these mutant cells showed a reduced ability to stimulate cytokine production by human PBMCs, dendritic cells, and macrophages (Table 1) [63,100,101,102]. In Candida parapsilosis, another opportunistic pathogen, loss of OCH1 caused large changes in the cell morphology, cell wall composition, and susceptibility to cell wall perturbing agents (Table 1) [65]. As with C. albicans, the C. parapsilosis och1Δ null mutant showed a reduced ability to stimulate cytokine production by human PBMCs, and virulence attenuation in neonate and adult murine models of systemic infections, but not in the Galleria mellonella invertebrate model (Table 1) [65,66]. In S. schenckii, OCH1 silencing caused minimal changes in the cell phenotype, with only subtle changes in the cell wall composition (Table 1) [64]. However, it was reported that as a compensatory mechanism for the loss of the N-linked glycan outer chain, these mutants increased the cell wall O-linked glycan content [64,103]. The contrasting phenotype between these mutant strains and those generated in C. albicans is likely because glycoproteins that have a high mannose content may not be the most abundant element in the S. schenckii cell wall [64]. Nevertheless, silenced strains showed virulence attenuation in a murine model of systemic infection and the invertebrate models G. mellonella and Tenebrio molitor [64,67] and had a diminished ability to stimulate proinflammatory cytokines when interacting with PBMCs, but an increased ability to stimulate IL-10 (Table 1) [64]. Contrasting these observations, the A. fumigatus och1Δ mutant did not show significant changes in growth or the cell wall composition, but a defect in its ability to sporulate (Table 1) [68]. Virulence analysis in a murine model did not show virulence attenuation, suggesting that fully extended N-linked glycans are not associated with A. fumigatus virulence [68,69]. In C. neoformans, OCH1 disruption slightly attenuated virulence when compared to the control strain (Table 1) [70]. These data suggest that, despite the fact the gene has a conserved role in protein glycosylation, it has a species-specific impact on the host–fungus interaction, suggesting that fully extended N-linked glycans may play different roles on the surfaces of medically relevant fungal species.
Many mannosyltransferases participate in the N-linked glycosylation process, and members of the MNN families fulfill different functions within the pathway [13,18]. The C. albicans MNN1 family encodes α-1,3-mannosyltransferases that add terminal mannose residues to the branches of the N-linked glycan outer chain and is composed of six members, MNN1, MNN12, MNN13, MNN14, MNN15, and MNN16 [71]. Null mutants of the members of the MNN1 family have been generated, and it was observed that all mutants had similar growth rates compared to the wild-type strain, with no obvious changes in the morphology and cell wall composition, suggesting functional redundancy [71]. The only exception was MNN14, where the null mutant showed a subtle defect in the cell wall composition, indicating that it is the dominant member of the gene family (Table 1) [71]. Despite the fact the mnn14Δ mutant phenotype was not as severe as the one reported for OCH1, ROT2, or CHW41 disruption, the mutant showed virulence attenuation in a murine model of disseminated candidiasis but did not show defects in cytokine production [71], suggesting that the structural changes in the N-linked glycans were not sufficient to disrupt the ligand–receptor interactions between the fungal and immune cell surfaces but affected the cellular fitness to display full virulence. As previously mentioned, the MNN2 gene family encodes α-1,2-mannosyltransferases involved in the synthesis of the backbone lateral chains of the N-linked glycan outer chain and is composed of MNN2, MNN21, MNN22, MNN23, MNN24, and MNN26 [72]. Mutants of this family showed changes in phenotype, and it was established that Mnn2 and Mnn26 are necessary for the addition of the initial α-1,2-mannose residue to the α-1,6-mannose backbone, and Mnn21, Mnn22, Mnn23, and Mnn24 are required to add α-1,2-mannose units to the α-1,6-α-1,2-mannose scaffold [72]. The absence of these genes caused severe virulence attenuation in both G. mellonella and murine models of systemic infection, suggesting the lateral chains of the N-linked glycan outer chain backbone are relevant for the pathogen–host interaction (Table 1) [72]. Similar to C. albicans, in C. neoformans, MNN2 encodes for a mannosyltransferase that adds mannose units to the N-linked glycan outer chain [70]. However, its contribution to the C. neoformans interaction with the host has not been elucidated yet.
In C. albicans, there is an extensive family of MNN4-like genes that is composed of MNN41, MNN42, MNN43, MNN44, MNN45, MNN46, and MNN47, which are presumed to be required for phosphomannan production [30]. Disruption of this gene family did not show any phenotype different from that described for MNN4, suggesting this gene is the dominant member of the gene family [30]. Loss of Mnn4 did not affect virulence in a murine model of systemic candidiasis, but the null mutant showed virulence attenuation in G. mellonella larvae, similar to the Candida tropicalis mnn4Δ null mutant, suggesting that phosphomannosylation is required to adapt to this host milieu [73,74]. Furthermore, loss of MNN4 led to changes in the C. albicans–macrophage interaction, where fungal uptake was reduced, and to increased resistance to antimicrobial peptides, stressing the relevance of this glycan moiety in the C. albicans–immune effector interaction (Table 1) [30,73,104,105].
Another important mannosyltransferase that participates in protein glycosylation is the α-1,2-mannosyltransferase Mnn5, which is regulated by iron in C. albicans [75]. An mnn5Δ null mutant showed hypersensitivity to wall perturbing agents, inability to undergo dimorphism, and virulence attenuation in a murine model of systemic candidiasis (Table 1) [75].
After the addition of the first α-1,6-mannose residue to the backbone of the N-linked glycan outer chain by Och1, Mnn9 is thought to be the major contributor to the α-1,6 backbone extension [76]. Mutations in its encoding gene led to changes in the fungal cell wall, osmotic instability, slow growth rates, defective hyphae, and severe cell agglutination (Table 1) [76]. Despite the fact the virulence of this mutant strain has not been evaluated, it is suggested that it may show virulence attenuation, as with cells lacking Och1 or other relevant mannosyltransferases involved in N-linked glycan elaboration. In A fumigatus, Mnn9 is also a GDP-Man-dependent α-1,6-mannosyltransferase, and disruption of the encoding gene led to cell wall integrity defects, changes in morphogenesis, and sensitivity to some wall perturbing agents (Table 1) [77]; however, its relevance in the Aspergillus–host interaction remains to be investigated. Similar to Mnn9, Mnn10 is an α-1,6-mannosyltransferase responsible for the elongation of the N-linked glycan outer chain backbone. In C. albicans, MNN10 deletion resulted in an abnormal organization of the cell wall, with a lower mannose content and increased exposure of β-1,3-glucan on the cell surface [78]. MNN10 was shown to be required for C. albicans pathogenicity in a murine model of systemic infection; however, the null mutant did not show defects in the ability to invade the host tissues (Table 1) [78]. Instead, the diminished virulence was attributed to greater immunological recognition of the mutant strain by the host [78]. MNN11 encodes for an α-1,6-mannosyltransferase involved in the final stage of N-linked glycoprotein elaboration [13]. Interestingly, disruption of this gene in Candida glabrata did not affect virulence (Table 1) [79].
Another important family for both N-linked and O-linked protein glycosylation is the KRE2/MNT1 family, which encodes a set of five Golgi-resident type-II α-mannosyltransferases in C. albicans [31,32,34]. Mnt3, Mnt4, and Mnt5 play a role in N-linked glycosylation [31], while Mnt1 and Mnt2 play a role in the synthesis of O-linked glycans [34]; the latter will be addressed in the following section. A triple mutant, mnt3-mnt4-mnt5Δ, exhibited an aggregated phenotype, increased susceptibility to cell wall perturbing agents, a significant reduction in the ability to stimulate cytokine production by PBMCs, and virulence attenuation in a murine model of systemic candidiasis (Table 1) [31]. These data indicate that this gene family is as relevant for interaction with the host as the MNN gene families involved in N-linked glycan synthesis.
In C. albicans, the BMT gene family is composed of nine members, BMT1-9, and encodes for β-mannosyltransferases responsible for adding β-mannose to the phosphopeptidomannan (PPM) [29]. The bmtΔ null mutants did not show any defects in phenotype or growth, nor were they sensitive to chemical or antifungal compounds. Despite the fact that all strains showed an altered pattern of β-mannose epitopes in PPM [29], more studies are needed to determine the relevance of these genes in C. albicans pathogenicity.
Another gene that is involved in both N-linked and O-linked glycosylation is PMR1. In C. albicans, it is known that this gene acts as a Golgi-resident P-type ATPase ion pump and is responsible for providing the Mn2+ cofactor to mannosyltransferases [80]. The C. albicans pmr1Δ null mutant showed cell aggregation, defects in the cell wall composition, hypersensitivity to wall perturbing agents, constitutive activation of the cell wall integrity pathway, and attenuated virulence in a murine model of infection, all mice survived throughout the experiment, and colonization in the organs by the mutant was not observed (Table 1) [80]. This indicates that PMR1 is essential for C. albicans virulence. In Candida guilliermondii, a low-virulence species, PMR1 disruption affected cell growth and morphology, the cell wall, biofilm formation, and the interaction with PBMCs and, as expected, led to virulence attenuation in both G. mellonella and mice (Table 1) [81]. In A. fumigatus, pmrA disruption generated defects in growth, and changes in the wall composition, presenting an increased content of β-glucan and chitin [82]. Additionally, the null mutant was hypersensitive to cell wall inhibitors, but its virulence was not attenuated in a murine model of invasive aspergillosis [82], suggesting that PmrA is necessary for the A. fumigatus cell wall integrity but not for virulence and pathogenesis (Table 1). Other genes that are related to the glycosylation processes of C. albicans are RER2, SRT1, and CWH8. The Rer2 and Srt1 proteins represent cis prenyltransferases, which are responsible for carrying out the synthesis of the dolichol skeleton [106]. The RER2 absence hinders the synthesis of dolichol in C. albicans and causes defects in growth, dimorphism, and the formation of the cell wall. In addition, RER2 mutants are sensitive to antifungal agents [106]. Finally, CWH8 encodes a dolichol pyrophosphate phosphatase that acts as a carbohydrate transporter during N-linked glycosylation and is responsible for converting dolichol pyrophosphate to dolichol phosphate in fungi [107]. Gene deletion disrupted this recycling pathway and pleiotropically affected the cell phenotype [107].
Collectively, all these studies show that many of the genes involved in the N-linked protein glycosylation pathway are important for host interaction and virulence.

4.2. Relevance of O-Linked Glycosylation during Host–Pathogen Interaction

O-Linked glycosylation has been studied extensively in C. albicans. This organism possesses a gene family known as PMT, which encodes five protein mannosyltransferases, Pmt1, Pmt2, Pmt4, Pmt5, and Pmt6, which initiate this post-translational modification [33,84]. The importance of this gene family for virulence and morphology has been evaluated by generating null mutant strains. All the single null mutants, except for pmt5Δ, showed defects in morphogenesis, likely attributed to the lack of mannosylation and, consequently, to a loss of activity of the target Pmt proteins, which are necessary for the polarized growth (Table 1) [84]. These mutants also showed remarkable susceptibility to antifungal agents; in particular, pmt1Δ and pmt4Δ were hypersensitive to antifungal agents, indicating that both proteins are involved in processes and structures that lead to high resistance to antifungal drugs (Table 1) [84]. The mutants also showed hypersensitivity to cell wall perturbing agents and changes in the cell wall components [84], and virulence was severely affected in the pmt1Δ, pmt4Δ, and pmt6Δ mutants when used to challenge a mouse model of systemic infection (Table 1) [85,86]. Pmt2 is essential for growth, and the pmt5Δ mutant did not exhibit decreased virulence in a systemic candidiasis model, but in other models of localized and systemic infection, it was demonstrated that PMT5 has an incomplete but significant effect on C. albicans virulence [84,89]. Mutants in PMT1, PMT4, and PMT2 are partially defective in protease secretion, which could limit the fungal ability to use host proteins as a nitrogen source, thus affecting fungal growth and fitness [60]. In A. fumigatus, the PMT gene family comprises only three genes, Afpmt1, Afpmt2, and Afpmt4 [87,108]. Loss of Afpmt1 resulted in growth defects, changes in the cell wall, and defects in cell morphology and conidia formation (Table 1) [108]. Interestingly, despite a reduction of about 60% in the O-linked glycan content, the Afpmt1Δ mutant showed no defects in virulence when analyzed in a mouse model of infection [108], and a similar observation has been reported for Afpmt4 (Table 1) [87]. In C. neoformans, the PMT family is also composed of three members, PMT1, PMT2, and PMT4 [88]. Disruption of PMT4 generated dramatic effects on fungal virulence, morphological defects, and alterations in the cell wall [88,109]. PMT1 disruption led to virulence attenuation in a macrophage death model and a murine model of cryptococcosis by inhalation (Table 1) [88].
As mentioned previously, another gene family important for O-linked glycosylation is KRE2/MNT1. From this, only MNT1 and MNT2 participate in this biosynthetic pathway in C. albicans [34]. The mnt1Δ null mutant showed changes in cell morphology, an inability to adhere to human buccal epithelial cells, hypersensitivity to wall perturbing agents, and virulence attenuation in a murine model of systemic infection (Table 1) [34,90]. The mnt2Δ null mutant exhibited a less marked phenotype than the mnt1Δ mutant but showed virulence attenuation (Table 1) [34]. In A. fumigatus, loss of Afmnt1 reduced the cell wall thickness and decreased cell growth, the ability to form conidia, and virulence in a murine infection model (Table 1) [110]. C. neoformans appears to have only one MNT1 ortholog, but this has not been characterized [50].
Thus, it can be concluded that O-linked glycosylation is an important event for cell fitness and for displaying full virulence.

5. Relevance of Glycosylphosphatidylinositol Anchors during Host–Pathogen Interaction

GPI biosynthesis has been reported to be important not only for the integrity of the cell wall but also for the fungal ability to cause damage to the host [6,111]. To date, only a few genes that participate in this pathway have been reported to be important for the host–fungus interaction. GWT1 and MCD4 have been described as part of the C. albicans GPI synthesis. GWT1 codes for an inositol acyltransferase that catalyzes the inositol acylation of glucosamine phosphatidylinositol (GlcN-PI) in the first step on the ER luminal side, having an important function in maintaining the cell wall integrity and enabling fungal adhesion to the host [6,91,92]. MCD4 codes for a mannose-ethanolamine phosphotransferase that adds a phosphoethanolamine on the first mannose of the GPI core [6,92]. When the activity of these proteins is disrupted by GPI inhibitors in Candida spp., Aspergillus spp., Fusarium spp., and Scedosporium spp., a defective phenotype in the assembly, translocation, and maturation of GPI proteins is observed, affecting the cell wall integrity and structure, growth, dimorphism, and virulence (Table 1) [93,94]. For example, when C. albicans is treated with BIQ, a Gwt1 inhibitor, the mannoprotein expression in the cell surface is blocked, therefore inhibiting fungal adhesion to a monolayer of mammalian epithelial cells [112]. Gene overexpression overcame the defective phenotype, and downregulation of expression caused an increase in the sensibility to the inhibitor, obtaining a phenotype with reduced growth and morphological changes [112]. Additionally, when C. albicans is treated with E1210, another Gwt1 inhibitor, a reduced expression of GPI-anchored adhesins that are important virulence factors, such as members of the Als family, is observed, with additional defects in germ tube and biofilm formation [92]. When gepinacin is used, a similar effect is observed, since the target for this inhibitor is the same, causing a phenotype with reduced adherence, growth, dimorphism, and secretion of lytic enzymes (Table 1) [6]. Gepinacin causes this effect by stressing the ER due to a blockage of the GPI-anchored trafficking [6]. Several inhibitors have also been reported for Mcd4, including M743 and M720, that, although being highly efficient as antifungals, also inhibit GPI biosynthesis in mammalian cells [113].
From all of the known S. cerevisiae genes that participate in GPI synthesis, only one has been proven to be non-essential, GPI7, whose deletion caused cell wall and mating defects [114]. This gene was later studied in C. albicans, and it was found that deletion mutants showed defects in chlamydospore formation and dimorphism on solid media but normal growth in liquid [95]. The cell wall, cytokinesis, and cell shape were affected in both solid and liquid media, and in vivo assays showed a reduced virulence due to an inability to colonize the gastrointestinal tract in a mouse model of disseminated candidiasis [95]. Additionally, a higher sensibility to the macrophages’ lytic action was observed, probably due to a downregulation of Erk1/2 phosphorylation after phagocytosis [95]. The addition of ethanolamine phosphate on the second mannose of the GPI anchor was later proved to be essential for the transport and localization of the cell wall GPI proteins in A. fumigatus [6]. As in C. albicans, deletion of gpi7 caused abnormal polarity and growth, altered levels of plasma membrane GPI proteins, incorrect localization of GPI proteins, and autophagy, but unlike C. albicans, no defects in the cell wall nor accumulation of GPI proteins in the ER were observed, and only the production of enlarged vacuoles and autophagosomes were present in the mutants, which are mechanisms to avoid ER stress and cell death (Table 1) [115].
C. albicans GPI2 and GPI19, accessory subunits from the GPI-GnT complex, participate in morphogenesis and fungal cell wall biogenesis [116]. These two proteins have oppositive effects and negatively regulate each other: Gpi2 determines hyphal morphogenesis through Ras1 signaling, while Gpi19 controls ergosterol biosynthesis by downregulating ERG11 levels and modulates the azole response [117,118]. A third subunit has been described, GPI15, a master activator of GPI2 and GPI19, important for growth, cell wall integrity, and GPI synthesis in C. albicans. GPI15 mutants were hypofilamentous, ergosterol defective, and azole sensitive and exhibited the phenotypes of both GPI2 and GPI19 mutants [96]. Additionally, GPI15 mutants were less virulent in vitro, since this gene regulates hyphal filamentation and invasive growth, which are important for the establishment of the infection by C. albicans (Table 1) [96]. Thus, by controlling Gpi12 and Gpi19 activities, Gpi15 may be a good antifungal target.
Unlike what has been seen in S. cerevisiae, in A. fumigatus, the loss of GPI anchors is not lethal. PigA (Gpi3), the catalytic subunit of the GPI-GnT complex, is necessary for cell wall synthesis and virulence, as demonstrated by the generation of null mutants [97]. These mutants were capable of growing at a temperature from 30 to 50 °C, being the first report of a GPI deficiency that is not temperature sensitive, although their growth rate was significantly slower [97]. The mutant cells were more sensitive to SDS, which suggested an unstable or damaged cell membrane, and had increased levels of the chitinase ChiB, which is responsible for the degradation of chitin during autolysis (Table 1) [97]. Finally, reduced virulence was observed in a mouse model of invasive aspergillosis, probably caused by the lack of important virulence factors such as Ecm33 and Gel2 [97,119].
GPI synthesis has also been implicated, although indirectly, in C. neoformans virulence, as demonstrated by gene expression analyses. When C. neoformans was grown under iron limitation conditions, elevated expression was observed for several genes, including CGP60, a gene essential for capsule production, FTR1, an iron permease, and GPI8 that encodes a GPI-transamidase [120]. When C. neoformans GPI8 disruption was attempted, no mutant alleles were obtained in 192 transformants, suggesting that GPI8 is essential for this organism [120]. This could be explained by the fact that the capsule is bound to cell wall components that can be affected by the lack of GPI8, suggesting this protein is important during cryptococcal infection [120].

6. Relevance of Non-Conventional Glycosylation Processes during Host–Pathogen Interaction

In the A. fumigatus glfBΔ mutant, the absence of galactofuranose showed a clear link between galactomannan and interaction with the host. The phenotype of the glfBΔ mutant was similar to the umg1Δ mutant, where poor growth was observed at the mammalian body temperature, showing attenuated virulence in a mouse model of invasive aspergillosis (Table 1) [45,98]. The umg1Δ mutants also showed a thinner cell wall that could be related to greater susceptibility to antifungal agents [98]. Disruption of A. fumigatus ugm1 led to the elimination of galactofuranose synthesis, and cells became hyper-adherent to inherent surfaces and also to pulmonary epithelial cells [99]. This was explained by the fact that galactofuranose residues are capable of masking some polar groups during cell wall maturation, and therefore adherence can be moderated [99,121]. The contribution of this hyper-adherence to the virulence of the fungus is not clear, but it has been found that ugm1Δ mutants do not present changes in virulence in a murine model [99]; meanwhile, another study showed that these mutant cells had attenuated virulence in a murine model of invasive aspergillosis [98]. More studies are needed to determine whether Ugm1 is involved in A. fumigatus virulence. It is believed that galactomannan and galactofuranose do not fulfill the role of adhesins, but both can modulate the expression and exposure of other types of molecules that are responsible for mediating adherence in the fungus [121]. Loss of ktr4 and ktr7 led to an absence of galactomannan in the A. fumigatus cell wall, leading to severe phenotype defects, showing a hyperbranched mycelium, loss of polarity during conidia germination, and hyphal elongation [46]. In a murine model of invasive aspergillosis, it was determined that the absence of Ktr4 and Ktr7 severely affected virulence (Table 1) [46].
In C. neoformans, KTR3 disruption, the mannosyltransferase involved in O-linked glycan synthesis [54], increased the sensitivity to SDS and NaCl, and virulence was attenuated in a murine model of cryptococcosis [54]. This virulence defect could be associated with changes in the function of cell surface and secretory proteins, particularly those that are associated with host infection, as a result of O-linked glycan involvement (Table 1) [54].
Silencing of S. schenckii RmlD, involved in protein rhamnosylation, did not affect cell growth or morphology but affected the cell wall composition and organization [57]. The silenced mutant strains exhibited a different ability to stimulate cytokine production by human PBMCs, stimulating lower levels of TNFα and IL-6, but a higher production of IL-1β and IL-10, compared to the wild-type strain [57]. Moreover, the silenced mutants showed virulence attenuation in the G. mellonella infection model, indicating that rhamnose is required to kill the larvae (Table 1) [57].

7. Protein Glycosylation as Potential Targets of Antifungal Drugs

As mentioned previously, the fungal cell wall is attractive for finding new targets for antifungal drugs because the molecular nature of its compounds is different from the proteins, polysaccharides, and oligosaccharides found in the mammalian host, and because some of these are ubiquitous molecules found in fungal cell walls, such as glucans and chitin, providing the alternative to have wide-ranging targets. Because of this, the synthesis of β-1,3-glucan and chitin has been thoroughly studied, and we currently have antifungal drugs that target these metabolic pathways available in the market, or in experimental phases [122]. However, since the synthesis of N-linked and O-linked glycans and GPI involves enzymes that are not present in human cells, such as those participating in glycan elongation in the Golgi complex, and due to the fact that it also contains bypassing steps not found in fungal cells [13] and that the human and pathogen glycosidases have different sensitivities to inhibitors [123], these biosynthetic pathways have also been analyzed as potential antifungal targets.
Rhodanine-derived benzylidene thiazolidinediones have shown mannosyltransferase inhibition that prevents the synthesis of fully extended O-linked mannans in C. albicans, and as a consequence, cells lost wall integrity and viability [122]. The derivative compound 5-[[3-(1-phenylethoxy)-4-(2-phenylethoxy)phenyl]methylene]-4-oxo-2-thioxo-3-thiazolidineacetic acid showed an in vitro ability to inhibit C. albicans Pmt1 and fungal proliferation, with an IC50 of 0.2–0.5 µM [124]. Despite the fact that human cells contain POMT1 and POMT2, enzymes with Dol-P-Man:protein O-D-mannosyltransferase activity, similar to that described for C. albicans Pmt1, the primary structure for these enzymes has 31 and 37% identity when compared with the fungal enzyme, suggesting similar motifs for enzyme activity but different conformations of catalytic pockets [125]. However, the effect of these compounds on human cells and in in vivo conditions remains to be addressed.
MOL3, a small molecule designed by computational docking, showed the ability to inhibit Paracoccidioides lutzii Kre2 and C. albicans Mnt1, both proteins that are involved in O-linked glycan synthesis [126]. With the exception of C. glabrata, this compound showed the ability to inhibit the growth of other Candida species [126]. When tested on C. parapsilosis, the minimal fungicidal concentration of MOL3 varied from 16 to 128 mg/L, depending on the analyzed strain [126]. When cells were incubated with 32 mg/L MOL3, they showed defects in the cell wall structure, absence of pseudohyphae when dimorphism was stimulated, and loss of cell metabolism [126]. In one cell line, MOL3 showed 30% cytotoxicity at 256 mg/L, and this was reduced when lower concentrations of the compound were incubated with the cell line, suggesting low cytotoxicity, and in vivo assays with 0.05 g/kg MOL3 applied to mice did not show any metabolic change suggestive of cytotoxicity [126]. Finally, MOL3 showed the ability to reduce the C. parapsilosis load in a murine model of systemic infection [126]. Collectively, these data make this small molecule a promising therapeutic candidate for the treatment of fungal infections.
Wortmannin has been isolated from Penicillium radicum and has shown antifungal activity against a vast range of medically relevant fungal species [127]. Despite being a phosphoinositide 3-kinase (PI3K) inhibitor, molecular docking analyses have provided confident data that suggest that this molecule may have other molecular targets, including the Kre2/Mnt1 Golgi α-1,2-mannosyltransferase [127], an enzyme relevant for O-linked glycan elongation. However, confirmation of this bioinformatically generated prediction is required to unveil the potential of this compound as an inhibitor of a glycosylation process.
Pradimicins A, B, C, D, and E are polyketides produced by the actinomycete Actinomadura hibisca, while benanomicins are produced by Actinoallomurus spadix [128,129]. Both types of nonribosomal peptides have shown antifungal activity in a mechanism that involves recognition of cell wall mannose, in a calcium-dependent mechanism that leads to apoptosis-like fungal cell death [130,131,132]. Interestingly, pradimicins have shown both in vitro and in vivo abilities to kill C. albicans, A. fumigatus, and C. neoformans [133]. Pradimicin A showed efficacy against experimental pulmonary candidiasis and aspergillosis, vaginal candidiasis, and superficial infections caused by Trichophyton mentagrophytes [133]. This compound was not toxic for mammalian cells at concentrations up to 500 µg/mL, which surpasses the LD50 of 400 mg/kg [133]. Benanomicin A showed in vivo antifungal activity against Pneumocystis carinii pneumonia when administered at 10 mg/kg [134].
Helja, a sunflower mannose-binding lectin belonging to the jacalin family, binds to C. albicans cell wall mannoproteins and, at a 200 μg/mL concentration, inhibits C. albicans, C. parapsilosis, and C. tropicalis growth [135]. The antifungal mechanism involves the disruption of the cell wall integrity, the inhibition of dimorphism, adhesion and biofilm formation, and the production of radical oxygen species [135,136].
Fosmanogepix (APX001), an N-phosphonooxymethyl prodrug of APX001A, targets Gwt1, an enzyme involved in the early steps of GPI anchor synthesis [112,137]. As a consequence, cell wall proteins do not reach the final destination within the cell wall, affecting both the cell wall integrity and fungal growth [92,137]. Thus far, this compound has shown antifungal activity against C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, Candida krusei, Candida dubliniensis, Candida lusitaniae, C. guilliermondii, Malassezia furfur, C. neoformans, and A. fumigatus, with MICs ranging <0.008–0.03 µg/mL and 0.03–0.13 µg/mL for Candida spp. and A. fumigatus, respectively [93]. Moreover, fosmanogepix did not affect the activity of the Gwt1 human ortholog, and thus inositol acylation of GPI occurred in its presence, suggesting selectivity against the fungal enzyme [92]. Since fosmanogepix prevents experimental fungal infections and has been successful in clinical phase 1 studies, it is now in phase 2 clinical trials for invasive candidiasis and aspergillosis [138,139].
In addition to all these examples, it is relevant to mention that disruption of key genes involved in the protein glycosylation pathways led to changes in the cell wall that make cells more susceptible to the action of antifungal drugs, i.e., disruption of these post-translational modifications leads to hypersensitivity to antifungal drugs. Examples of these are disruption of A. fumigatus mnn9, mnt1, and pmt4 [77,87,110], C. glabrata MNN2 [140], C. albicans DPM1, DPM2, DPM3, ALG13, RER2, MNN10, PMT1, PMT2, and PMT4 [21,23,78,84], and S. cerevisiae DPM1 and SEC59 [141].
Despite all these examples of promising new targets for antifungal drugs, these observations have to be taken with caution, as these types of statements may not be generalized to all the players in the protein glycosylation pathways and all the fungal species, S. cerevisiae null mutants in the PMT gene family, particularly the pmt2Δ null mutant, showed increased tolerance to PAF26, a synthetic cationic antifungal hexapeptide antifungal [142]. Moreover, Mnn1, Mnn4, and Mnn5, Golgi-resident α-mannosyltransferases involved in the synthesis of both N-linked and O-linked glycans, are required for S. cerevisiae tolerance to PAF26 [142]. These observations are likely to occur because the defensins and PAF26 interact with glycosylated protein targets that need post-translational modifications to bind the antifungal compounds. For the case of the antimicrobial peptide P-113Tri, derived from human histatin 5, it requires the presence of Och1 activity to display anti-C. albicans activity [7]. This is because most of the C. albicans cell wall phosphomannan is attached to the N-linked mannan outer chain, and Och1 catalyzes the first step in its elaboration [28,31]. Loss of the N-linked outer chain implies loss of most of the phosphomannan, which confers the net negative charge of the cell surface, required for the binding and internalization of P-113Tri [7]. Disruption of C. albicans GPI7, a mannose-ethanolamine phosphotransferase that participates in GPI anchor synthesis, led to increments in the cell wall chitin, and as a consequence, cells showed increased resistance to caspofungin [143].

8. Protein Glycosylation as a Target for the Development of Antifungal Vaccines

Currently, no vaccine has been approved to treat fungal infections, and most of the experimental designs to generate immunological protection are based on epitopes that are part of key proteins for fungal pathogenesis or structural polysaccharides, such as β-1,3-glucans or chitosan [144,145]. However, some approaches that exploit the particular structure and composition of cell wall N-linked and O-linked glycans have been reported.
The β-1,2-mannotriose found as part of the C. albicans cell wall phosphomannan [24] was chemically synthesized and conjugated to the N-terminal end of the adhesin Als1 [146]. When injected into mice, this conjugate stimulated the production of a specific IgG against both the trisaccharide and the peptide, in an adjuvant-free event [146]. Since these antibodies recognized the trisaccharide present in the C. albicans, C. tropicalis, C. lusitaniae, and C. glabrata wall surface, it is thought that the trisaccharide alone or conjugated to Als1 could be a candidate for the development of a vaccine against these pathogens [146]. A similar approach has also been reported, conjugating this trisaccharide to fructose-bisphosphate aldolase and to β-1,3-linked hexaglucan, in order to provide epitopes that stimulate both B and T lymphocytes, thus improving the immunological properties of the conjugate [147]. The heptasaccharide β-D-Man-(1→2)-α-D-Man-(1→3)-α-DMan-(1→2)-α-D-Man-(1→3)-α-D-Man-(1→2) α-D-Man-(1→2)-α-D-Man, which belongs to the C. albicans antigenic factor 9, part of the N-linked glycan outer chain [24], was conjugated to bovine serum albumin and subcutaneously inoculated in mice [148]. After two boosts, this glycoconjugate elevated the antifungal activity of polymorphonuclear leukocytes, increased the phagocytic activity and respiratory burst of granulocytes, and elevated the subpopulations of CD3(+) T, CD4(+)CD25(+) T, and CD4(+)/CD8(+) T lymphocytes, promoting an increment in the levels of CD8(+)CD25(+) cells and a Th1-based, Th2-based, and Th17-based immune response [148]. A similar antigenic preparation (α-1, 6-linked N-linked glycan outer chain backbone highly branched with α-1,2-, α-1,3-, and β-1,2-linked mannooligomers) derived from C. albicans showed conferred protection to mice against a lethal dose of A. fumigatus [149]. This protection was achieved with a dose of 12 mg of mannan or with 0.3 mg when conjugated to bovine serum albumin [149]. Despite the fact that these different structures are capable of conferring resistance to antifungal infections, reverse engineering approaches have revealed that β-1,2-mannobiose conjugated to a protein is the minimal epitope to induce protective antibodies against a fungal infection [150]. Indeed, immunized rabbits with this glycoconjugate showed protection against C. albicans [150].
The C. albicans cell wall mannan extracted and encapsulated in liposomes or conjugated to a protein induced the production of protective antibodies in a murine model of systemic candidiasis, preventing animal death [151]. The mannan–protein conjugate conferred 100% protection to animals without a functional complement system, while encapsulated mannan in liposomes was capable of protecting only 60% of the animal population that received this formulation [151]. Since the administration of the mannan–protein conjugate showed superior protective levels in complement-deficient elements compared to liposomes, this formulation results in being more attractive for further development of an anti-Candida vaccine.
Recently, passive immunization with C3.1, an anti-β-1,2-mannotriose monoclonal antibody, showed protection in a murine model of systemic candidiasis by Candida auris [152], suggesting this could be an effective alternative in cases of resistance to antifungal drugs or for the treatment of immunocompromised patients.

9. Conclusions

In pathogenic fungi, there is a clear correlation between proper protein glycosylation and virulence. Many processes that are essential to establish diseases, such as adhesion, proliferation, dimorphism, evasion of the host immune response, and biofilm formation, depend on the correct synthesis of N-linked and O-linked glycans and GPI anchors, as demonstrated by the deletion of key genes participating in these biosynthesis pathways. In addition, glycosylation plays an important role in cell wall synthesis, a process that influences the cell shape, growth rate, and wall plasticity and contributes to general cell fitness. Therefore, knowledge about the genes and proteins involved in these metabolic routes is of particular relevance in medically relevant fungal species, as it can lead to developing new therapeutic approaches to treat mycoses. As exemplified here, protein glycosylation could be considered as a promising target not only for antifungal drugs but also for the development of antifungal vaccines. One imperative challenge in the short and middle terms is to analyze these pathways in other relevant fungal species. Despite the fact that our knowledge about glycan structures and enzymes in charge of their elaboration can be considered vast, it is narrow when listing the fungal species where these aspects have been studied. It is now clear that glycan structures cannot be extrapolated from one species to another, and a similar observation applies to the contribution of enzymes to glycan synthesis, underlining the need for more studies in this area.

Funding

This work was supported by Consejo Nacional de Ciencia y Tecnología (ref. FC 2015-02-834; Ciencia de Frontera 2019-6380), and Red Temática Glicociencia en Salud (CONACYT-México). The funding sources that supported this work did not have any involvement in the design, acquisition, and analysis of data, or the writing of the manuscript.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Lin, B.; Qing, X.; Liao, J.; Zhuo, K. Role of Protein Glycosylation in Host-Pathogen Interaction. Cells 2020, 9, 1022. [Google Scholar] [CrossRef]
  2. Kornfeld, R.; Kornfeld, S. Assembly of Asparagine-Linked Oligosaccharides. Annu. Rev. Biochem. 1985, 54, 631–664. [Google Scholar] [CrossRef]
  3. Herscovics, A.; Orlean, P. Glycoprotein biosynthesis in yeast. FASEB J. 1993, 7, 540–550. [Google Scholar] [CrossRef]
  4. Gavel, Y.; von Heijne, G.; Creaser, E.; Murali, C.; Britt, K. Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: Implications for protein engineering. Protein Eng. Des. Sel. 1990, 3, 433–442. [Google Scholar] [CrossRef]
  5. Tanner, W.; Lehle, L. Protein glycosylation in yeast. Biochim. Biophys. Acta Rev. Biomembr. 1987, 906, 81–99. [Google Scholar] [CrossRef]
  6. Yadav, U.; Khan, M.A. Targeting the GPI biosynthetic pathway. Pathog. Glob. Health. 2018, 112, 115–122. [Google Scholar] [CrossRef] [PubMed]
  7. Lin, G.-Y.; Chang, C.-F.; Lan, C.-Y. The interaction between Carbohydrates and the Antimicrobial Peptide P-113Tri is Involved in the Killing of Candida albicans. Microorganisms 2020, 8, 299. [Google Scholar] [CrossRef] [PubMed][Green Version]
  8. Gow, N.A.R.; Latge, J.-P.; Munro, C.A. The Fungal Cell Wall: Structure, Biosynthesis, and Function. Microbiol. Spectr. 2017, 5, 1–25. [Google Scholar] [CrossRef] [PubMed][Green Version]
  9. Díaz-Jiménez, D.F.; Pérez-García, L.A.; Martínez-Álvarez, J.A.; Mora-Montes, H.M. Role of the Fungal Cell Wall in Pathogenesis and Antifungal Resistance. Curr. Fungal Infect. Rep. 2012, 6, 275–282. [Google Scholar] [CrossRef]
  10. Netea, M.G.; Brown, G.D.; Kullberg, B.J.; Gow, N.A. An integrated model of the recognition of Candida albicans by the innate immune system. Nat. Rev. Microbiol. 2008, 6, 67–78. [Google Scholar] [CrossRef] [PubMed]
  11. Latgé, J.-P. Tasting the fungal cell wall. Cell. Microbiol. 2010, 12, 863–872. [Google Scholar] [CrossRef]
  12. Brown, G.D.; Denning, D.W.; Gow, N.A.R.; Levitz, S.M.; Netea, M.G.; White, T.C. Hidden Killers: Human Fungal Infections. Sci. Transl. Med. 2012, 4, 165rv13. [Google Scholar] [CrossRef][Green Version]
  13. Martinez-Duncker, I.; Díaz-Jímenez, D.F.; Mora-Montes, H.M. Comparative Analysis of Protein Glycosylation Pathways in Humans and the Fungal Pathogen Candida albicans. Int. J. Microbiol. 2014, 2014, 267497. [Google Scholar] [CrossRef][Green Version]
  14. Parodi, A.J. N-Glycosylation in trypanosomatid protozoa. Glycobiology 1993, 3, 193–199. [Google Scholar] [CrossRef]
  15. Burda, P.; Aebi, M. The dolichol pathway of N-linked glycosylation. Biochim. Biophys. Acta Gen. Subj. 1999, 1426, 239–257. [Google Scholar] [CrossRef]
  16. Lehle, L.; Strahl, S.; Tanner, W. Protein Glycosylation, Conserved from Yeast to Man: A Model Organism Helps Elucidate Congenital Human Diseases. Angew. Chem. Int. Ed. 2006, 45, 6802–6818. [Google Scholar] [CrossRef]
  17. Aebi, M. N-linked protein glycosylation in the ER. Biochim. Biophys. Acta Bioenerg. 2013, 1833, 2430–2437. [Google Scholar] [CrossRef][Green Version]
  18. Dean, N. Asparagine-linked glycosylation in the yeast Golgi. Biochim. Biophys. Acta Gen. Subj. 1999, 1426, 309–322. [Google Scholar] [CrossRef]
  19. Schwarz, F.; Aebi, M. Mechanisms and principles of N-linked protein glycosylation. Curr. Opin. Struct. Biol. 2011, 21, 576–582. [Google Scholar] [CrossRef] [PubMed]
  20. Chojnacki, T.; Dallner, G. The biological role of dolichol. Biochem. J. 1988, 251, 1–9. [Google Scholar] [CrossRef] [PubMed][Green Version]
  21. Juchimiuk, M.; Kruszewska, J.; Palamarczyk, G. Dolichol phosphate mannose synthase from the pathogenic yeast Candida albicans is a multimeric enzyme. Biochim. Biophys. Acta Gen. Subj. 2015, 1850, 2265–2275. [Google Scholar] [CrossRef]
  22. Samuelson, J.; Banerjee, S.; Magnelli, P.; Cui, J.; Kelleher, D.J.; Gilmore, R.; Robbins, P.W. The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases. Proc. Natl. Acad. Sci. USA 2005, 102, 1548–1553. [Google Scholar] [CrossRef] [PubMed][Green Version]
  23. Niewiadomska, M.; Janik, A.; Perlińska-Lenart, U.; Piłsyk, S.; Palamarczyk, G.; Kruszewska, J.S. The role of Alg13 N-acetylglucosaminyl transferase in the expression of pathogenic features of Candida albicans. Biochim. Biophys. Acta Gen. Subj. 2017, 1861, 789–801. [Google Scholar] [CrossRef] [PubMed]
  24. Mora-Montes, H.M.; Ponce-Noyola, P.; Villagómez-Castro, J.C.; Gow, N.A.; Flores-Carreón, A.; López-Romero, E. Protein glycosylation in Candida. Futur. Microbiol. 2009, 4, 1167–1183. [Google Scholar] [CrossRef] [PubMed]
  25. Yan, Q.; Lennarz, W.J. Oligosaccharyltransferase: A Complex Multisubunit Enzyme of the Endoplasmic Reticulum. Biochem. Biophys. Res. Commun. 1999, 266, 684–689. [Google Scholar] [CrossRef]
  26. Mora-Montes, H.M.; Bates, S.; Netea, M.G.; Díaz-Jiménez, D.F.; López-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Kullberg, B.J.; Brown, A.J.P.; Odds, F.C.; et al. Endoplasmic Reticulum α-Glycosidases of Candida albicans Are Required for N Glycosylation, Cell Wall Integrity, and Normal Host-Fungus Interaction. Eukaryot. Cell 2007, 6, 2184–2193. [Google Scholar] [CrossRef][Green Version]
  27. Nishikawa, A.; Poster, J.B.; Jigami, Y.; Dean, N. Molecular and Phenotypic Analysis of CaVRG4, Encoding an Essential Golgi Apparatus GDP-Mannose Transporter. J. Bacteriol. 2002, 184, 29–42. [Google Scholar] [CrossRef][Green Version]
  28. Bates, S.; Hughes, H.B.; Munro, C.; Thomas, W.P.; MacCallum, D.; Bertram, G.; Atrih, A.; Ferguson, M.; Brown, A.J.; Odds, F.C.; et al. Outer Chain N-Glycans Are Required for Cell Wall Integrity and Virulence of Candida albicans. J. Biol. Chem. 2006, 281, 90–98. [Google Scholar] [CrossRef][Green Version]
  29. Mille, C.; Bobrowicz, P.; Trinel, P.-A.; Li, H.; Maes, E.; Guerardel, Y.; Fradin, C.; Martínez-Esparza, M.; Davidson, R.C.; Janbon, G.; et al. Identification of a New Family of Genes Involved in β-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans. J. Biol. Chem. 2008, 283, 9724–9736. [Google Scholar] [CrossRef][Green Version]
  30. González-Hernández, R.J.; Jin, K.; Hernández-Chávez, M.J.; Díaz-Jiménez, D.F.; Trujillo-Esquivel, E.; Clavijo-Giraldo, D.M.; Tamez-Castrellón, A.K.; Franco, B.; Gow, N.A.R.; Mora-Montes, H.M. Phosphomannosylation and the Functional Analysis of the Extended Candida albicans MNN4-Like Gene Family. Front. Microbiol. 2017, 8, 2156. [Google Scholar] [CrossRef][Green Version]
  31. Mora-Montes, H.M.; Bates, S.; Netea, M.G.; Castillo, L.; Brand, A.; Buurman, E.T.; Díaz-Jiménez, D.F.; Kullberg, B.J.; Brown, A.J.; Odds, F.C.; et al. A Multifunctional Mannosyltransferase Family in Candida albicans Determines Cell Wall Mannan Structure and Host-Fungus Interactions. J. Biol. Chem. 2010, 285, 12087–12095. [Google Scholar] [CrossRef] [PubMed][Green Version]
  32. Díaz-Jiménez, D.F.; Mora-Montes, H.M.; Hernández-Cervantes, A.; Luna-Arias, J.P.; Gow, N.A.; Flores-Carreón, A. Biochemical characterization of recombinant Candida albicans mannosyltransferases Mnt1, Mnt2 and Mnt5 reveals new functions in O- and N-mannan biosynthesis. Biochem. Biophys. Res. Commun. 2012, 419, 77–82. [Google Scholar] [CrossRef] [PubMed][Green Version]
  33. Goto, M. ProteinO-Glycosylation in Fungi: Diverse Structures and Multiple Functions. Biosci. Biotechnol. Biochem. 2007, 71, 1415–1427. [Google Scholar] [CrossRef] [PubMed][Green Version]
  34. Munro, C.; Bates, S.; Buurman, E.T.; Hughes, H.B.; MacCallum, D.; Bertram, G.; Atrih, A.; Ferguson, M.; Bain, J.M.; Brand, A.; et al. Mnt1p and Mnt2p of Candida albicans Are Partially Redundant α-1,2-Mannosyltransferases That Participate in O-Linked Mannosylation and Are Required for Adhesion and Virulence. J. Biol. Chem. 2005, 280, 1051–1060. [Google Scholar] [CrossRef] [PubMed][Green Version]
  35. Pittet, M.; Conzelmann, A. Biosynthesis and function of GPI proteins in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 2007, 1771, 405–420. [Google Scholar] [CrossRef][Green Version]
  36. Newman, H.A.; Romeo, M.J.; Lewis, S.E.; Yan, B.C.; Orlean, P.; Levin, D.E. Gpi19, the Saccharomyces cerevisiae Homologue of Mammalian PIG-P, Is a Subunit of the Initial Enzyme for Glycosylphosphatidylinositol Anchor Biosynthesis. Eukaryot. Cell 2005, 4, 1801–1807. [Google Scholar] [CrossRef][Green Version]
  37. Grimme, S.J.; Colussi, P.A.; Taron, C.H.; Orlean, P. Deficiencies in the essential Smp3 mannosyltransferase block glycosylphosphatidylinositol assembly and lead to defects in growth and cell wall biogenesis in Candida albicans. Microbiology 2004, 150, 3115–3128. [Google Scholar] [CrossRef][Green Version]
  38. Peter, O.; Menon, A. Thematic review series: Lipid Posttranslational Modifications. GPI anchoring of protein in yeast and mammalian cells, or: How we learned to stop worrying and love glycophospholipids. J. Lipid Res. 2007, 48, 993–1011. [Google Scholar] [CrossRef][Green Version]
  39. Fraering, P.; Imhof, I.; Meyer, U.; Strub, J.-M.; Van Dorsselaer, A.; Vionnet, C.; Conzelmann, A. The GPI Transamidase Complex of Saccharomyces cerevisiae Contains Gaa1p, Gpi8p, and Gpi16p. Mol. Biol. Cell 2001, 12, 3295–3306. [Google Scholar] [CrossRef][Green Version]
  40. Kudoh, A.; Okawa, Y.; Shibata, N. Significant structural change in both O- and N-linked carbohydrate moieties of the antigenic galactomannan from Aspergillus fumigatus grown under different culture conditions. Glycobiology 2014, 25, 74–87. [Google Scholar] [CrossRef][Green Version]
  41. Latgé, J.P.; Mouyna, I.; Tekaia, F.; Beauvais, A.; Debeaupuis, J.P.; Nierman, W. Specific molecular features in the organization and biosynthesis of the cell wall ofAspergillus fumigatus. Med. Mycol. 2005, 43, 15–22. [Google Scholar] [CrossRef] [PubMed][Green Version]
  42. Tefsen, B.; Ram, A.F.J.; Van Die, I.; Routier, F. Galactofuranose in eukaryotes: Aspects of biosynthesis and functional impact. Glycobiology 2011, 22, 456–469. [Google Scholar] [CrossRef][Green Version]
  43. Katafuchi, Y.; Li, Q.; Tanaka, Y.; Shinozuka, S.; Kawamitsu, Y.; Izumi, M.; Ekino, K.; Mizuki, K.; Takegawa, K.; Shibata, N.; et al. GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus. Glycobiology 2017, 27, 568–581. [Google Scholar] [CrossRef] [PubMed]
  44. Komachi, Y.; Hatakeyama, S.; Motomatsu, H.; Futagami, T.; Kizjakina, K.; Sobrado, P.; Ekino, K.; Takegawa, K.; Goto, M.; Nomura, Y.; et al. gfsAencodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen ofO-glycan inAspergillus nidulansandAspergillus fumigatus. Mol. Microbiol. 2013, 90, 1054–1073. [Google Scholar] [CrossRef] [PubMed][Green Version]
  45. Engel, J.; Schmalhorst, P.; Dörk, T.; Ferrieres, V.; Routier, F.H. A Single UDP-galactofuranose Transporter Is Required for Galactofuranosylation in Aspergillus fumigatus. J. Biol. Chem. 2009, 284, 33859–33868. [Google Scholar] [CrossRef][Green Version]
  46. Henry, C.; Li, J.; Danion, F.; Alcazar-Fuoli, L.; Mellado, E.; Beau, R.; Jouvion, G.; Latgé, J.-P.; Fontaine, T. Two KTR Mannosyltransferases Are Responsible for the Biosynthesis of Cell Wall Mannans and Control Polarized Growth in Aspergillus fumigatus. mBio 2019, 10, e02647-18. [Google Scholar] [CrossRef][Green Version]
  47. Onoue, T.; Tanaka, Y.; Hagiwara, D.; Ekino, K.; Watanabe, A.; Ohta, K.; Kamei, K.; Shibata, N.; Goto, M.; Oka, T. Identification of Two Mannosyltransferases Contributing to Biosynthesis of the Fungal-type Galactomannan α-Core-Mannan Structure in Aspergillus fumigatus. Sci. Rep. 2018, 8, 16918. [Google Scholar] [CrossRef] [PubMed]
  48. Latgé, J.-P.; Beauvais, A.; Chamilos, G. The Cell Wall of the Human Fungal PathogenAspergillus fumigatus: Biosynthesis, Organization, Immune Response, and Virulence. Annu. Rev. Microbiol. 2017, 71, 99–116. [Google Scholar] [CrossRef]
  49. Henry, C.; Fontaine, T.; Heddergott, C.; Robinet, P.; Aimanianda, V.; Beau, R.; Beauvais, A.; Mouyna, I.; Prevost, M.-C.; Fekkar, A.; et al. Biosynthesis of cell wall mannan in the conidium and the mycelium ofAspergillusfumigatus. Cell. Microbiol. 2016, 18, 1881–1891. [Google Scholar] [CrossRef][Green Version]
  50. Klutts, J.S.; Yoneda, A.; Reilly, M.C.; Bose, I.; Doering, T.L. Glycosyltransferases and their products: Cryptococcal variations on fungal themes. FEMS Yeast Res. 2006, 6, 499–512. [Google Scholar] [CrossRef][Green Version]
  51. Rodrigues, M.; Dobroff, A.S.S.; Couceiro, J.N.D.S.S.; Alviano, C.S.; Schauer, R.; Travassos, L.R. Sialylglycoconjugates and sialyltransferase activity in the fungus Cryptococcus neoformans. Glycoconj. J. 2002, 19, 165–173. [Google Scholar] [CrossRef] [PubMed]
  52. Doering, T.L. How Sweet it is! Cell Wall Biogenesis and Polysaccharide Capsule Formation inCryptococcus neoformans. Annu. Rev. Microbiol. 2009, 63, 223–247. [Google Scholar] [CrossRef] [PubMed][Green Version]
  53. Schutzbach, J.; Ankel, H.; Brockhausen, I. Synthesis of cell envelope glycoproteins of Cryptococcus laurentii. Carbohydr. Res. 2007, 342, 881–893. [Google Scholar] [CrossRef][Green Version]
  54. Lee, D.-J.; Bahn, Y.-S.; Kim, H.-J.; Chung, S.-Y.; Kang, H.A. Unraveling the Novel Structure and Biosynthetic Pathway of O-Linked Glycans in the Golgi Apparatus of the Human Pathogenic Yeast Cryptococcus neoformans. J. Biol. Chem. 2015, 290, 1861–1873. [Google Scholar] [CrossRef][Green Version]
  55. Reilly, M.; Aoki, K.; Wang, Z.A.; Skowyra, M.; Williams, M.; Tiemeyer, M.; Doering, T.L. A Xylosylphosphotransferase of Cryptococcus neoformans Acts in Protein O-Glycan Synthesis. J. Biol. Chem. 2011, 286, 26888–26899. [Google Scholar] [CrossRef][Green Version]
  56. Bezerra, L.L.L.-B.L. Sporothrix schenckii Cell Wall Peptidorhamnomannans. Front. Microbiol. 2011, 2, 243. [Google Scholar] [CrossRef][Green Version]
  57. Tamez-Castrellón, A.K.; van der Beek, S.L.; López-Ramírez, L.A.; Martínez-Duncker, I.; Lozoya-Pérez, N.E.; van Sorge, N.M.; Mora-Montes, H.M. Disruption of protein rhamnosylation affects the Sporothrix schenckii-host interaction. Cell Surf. 2021, 7, 100058. [Google Scholar] [CrossRef]
  58. Lopes-Bezerra, L.M.; Mora-Montes, H.M.; Zhang, Y.; Nino-Vega, G.; Rodrigues, A.M.; De Camargo, Z.P.; De Hoog, S. Sporotrichosis between 1898 and 2017: The evolution of knowledge on a changeable disease and on emerging etiological agents. Med. Mycol. 2018, 56, S126–S143. [Google Scholar] [CrossRef]
  59. Cutler, J.E. N-glycosylation of yeast, with emphasis on Candida albicans. Med Mycol. 2001, 39 (Suppl. S1), 75–86. [Google Scholar] [CrossRef][Green Version]
  60. Ernst, J.F.; Prill, S.K.-H. O-Glycosylation. Med. Mycol. 2001, 39, 67–74. [Google Scholar] [CrossRef][Green Version]
  61. Zhang, L.; Zhou, H.; Ouyang, H.; Li, Y.; Jin, C. Afcwh41is required for cell wall synthesis, conidiation, and polarity inAspergillus fumigatus. FEMS Microbiol. Lett. 2008, 289, 155–165. [Google Scholar] [CrossRef][Green Version]
  62. Brunke, S.; Quintin, J.; Kasper, L.; Jacobsen, I.D.; Richter, M.E.; Hiller, E.; Schwarzmüller, T.; d’Enfert, C.; Kuchler, K.; Rupp, S.; et al. Of mice, flies--and men? Comparing fungal infection models for large-scale screening efforts. Dis. Model Mech. 2015, 8, 473–486. [Google Scholar] [CrossRef][Green Version]
  63. Mukaremera, L.; Lee, K.K.; Mora-Montes, H.M.; Gow, N.A.R. Candida albicans Yeast, Pseudohyphal, and Hyphal Morphogenesis Differentially Affects Immune Recognition. Front. Immunol. 2017, 8, 629. [Google Scholar] [CrossRef][Green Version]
  64. Lozoya-Pérez, N.E.; Casas-Flores, S.; de Almeida, J.R.F.; Martínez-Álvarez, J.A.; López-Ramírez, L.A.; Jannuzzi, G.P.; Trujillo-Esquivel, E.; Estrada-Mata, E.; Almeida, S.R.; Franco, B.; et al. Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host–fungus interaction. Infect. Drug Resist. 2018, 12, 67–85. [Google Scholar] [CrossRef][Green Version]
  65. Perez-Garcia, L.A.; Csonka, K.; Flores-Carreon, A.; Estrada-Mata, E.; Mellado-Mojica, E.; Nemeth, T.; Lopez-Ramirez, L.A.; Toth, R.; Lopez, M.G.; Vizler, C.; et al. Role of protein glycosylation in Candida parapsilosis cell wall integrity and host interaction. Front. Microbiol. 2016, 7, 306. [Google Scholar] [CrossRef]
  66. Csonka, K.; Vadovics, M.; Marton, A.; Vágvölgyi, C.; Zajta, E.; Tóth, A.; Tóth, R.; Vizler, C.; Tiszlavicz, L.; Mora-Montes, H.M.; et al. Investigation of OCH1 in the Virulence of Candida parapsilosis Using a New Neonatal Mouse Model. Front. Microbiol. 2017, 8, 1197. [Google Scholar] [CrossRef] [PubMed][Green Version]
  67. Lozoya-Pérez, N.E.; García-Carnero, L.C.; Martínez-Álvarez, J.A.; Martínez-Duncker, I.; Mora-Montes, H.M. Tenebrio molitor as an Alternative Model to Analyze the Sporothrix Species Virulence. Infect. Drug Resist. 2021, 14, 2059–2072. [Google Scholar] [CrossRef] [PubMed]
  68. Kotz, A.; Wagener, J.; Engel, J.; Routier, F.H.; Echtenacher, B.; Jacobsen, I.; Heesemann, J.; Ebel, F. Approaching the Secrets of N-Glycosylation in Aspergillus fumigatus: Characterization of the AfOch1 Protein. PLoS ONE 2010, 5, e15729. [Google Scholar] [CrossRef] [PubMed][Green Version]
  69. Lambou, K.; Perkhofer, S.; Fontaine, T.; Latge, J.-P. Comparative functional analysis of the OCH1 mannosyltransferase families in Aspergillus fumigatus and Saccharomyces cerevisiae. Yeast 2010, 27, 625–636. [Google Scholar] [CrossRef] [PubMed]
  70. Park, J.-N.; Lee, D.-J.; Kwon, O.; Oh, D.-B.; Bahn, Y.-S.; Kang, H.A. Unraveling Unique Structure and Biosynthesis Pathway of N-Linked Glycans in Human Fungal Pathogen Cryptococcus neoformans by Glycomics Analysis. J. Biol. Chem. 2012, 287, 19501–19515. [Google Scholar] [CrossRef] [PubMed][Green Version]
  71. Bates, S.; Hall, R.A.; Cheetham, J.; Netea, M.G.; Maccallum, D.M.; Brown, A.J.; Odds, F.C.; Gow, N.A. Role of the Candida albicans MNN1 gene family in cell wall structure and virulence. BMC Res. Notes 2013, 6, 294. [Google Scholar] [CrossRef][Green Version]
  72. Hall, R.; Bates, S.; Lenardon, M.D.; MacCallum, D.; Wagener, J.; Lowman, D.W.; Kruppa, M.D.; Williams, D.L.; Odds, F.C.; Brown, A.J.; et al. The Mnn2 Mannosyltransferase Family Modulates Mannoprotein Fibril Length, Immune Recognition and Virulence of Candida albicans. PLoS Pathog. 2013, 9, e1003276. [Google Scholar] [CrossRef]
  73. Hernández-Chávez, M.J.; Franco, B.; Clavijo-Giraldo, D.M.; Hernández, N.V.; Estrada-Mata, E.; Mora-Montes, H.M. Role of protein phosphomannosylation in the Candida tropicalis–macrophage interaction. FEMS Yeast Res. 2018, 18, 18. [Google Scholar] [CrossRef]
  74. Hobson, R.; Munro, C.; Bates, S.; MacCallum, D.; Cutler, J.E.; Heinsbroek, S.E.M.; Brown, G.; Odds, F.C.; Gow, N. Loss of Cell Wall Mannosylphosphate in Candida albicans Does Not Influence Macrophage Recognition. J. Biol. Chem. 2004, 279, 39628–39635. [Google Scholar] [CrossRef][Green Version]
  75. Bai, C.; Xu, X.; Chan, F.-Y.; Lee, R.T.H.; Wang, Y. MNN5 Encodes an Iron-Regulated α-1,2-Mannosyltransferase Important for Protein Glycosylation, Cell Wall Integrity, Morphogenesis, and Virulence in Candida albicans. Eukaryot. Cell 2006, 5, 238–247. [Google Scholar] [CrossRef][Green Version]
  76. Southard, S.B.; Specht, C.A.; Mishra, C.; Chen-Weiner, J.; Robbins, P.W. Molecular Analysis of the Candida albicans Homolog of Saccharomyces cerevisiae MNN9, Required for Glycosylation of Cell Wall Mannoproteins. J. Bacteriol. 1999, 181, 7439–7448. [Google Scholar] [CrossRef] [PubMed][Green Version]
  77. Du, T.; Ouyang, H.; Voglmeir, J.; Wilson, I.B.; Jin, C. Aspergillus fumigatus Mnn9 is responsible for mannan synthesis and required for covalent linkage of mannoprotein to the cell wall. Fungal Genet. Biol. 2019, 128, 20–28. [Google Scholar] [CrossRef]
  78. Zhang, S.Q.; Zou, Z.; Shen, H.; Shen, S.S.; Miao, Q.; Huang, X.; Liu, W.; Li, L.P.; Chen, S.M.; Yan, L.; et al. Mnn10 Maintains Pathogenicity in Candida albicans by Extending α-1,6-Mannose Backbone to Evade Host Dectin-1 Mediated Antifungal Immunity. PLoS Pathog. 2016, 12, e1005617. [Google Scholar] [CrossRef] [PubMed]
  79. West, L.; Lowman, D.W.; Mora-Montes, H.M.; Grubb, S.; Murdoch, C.; Thornhill, M.H.; Gow, N.; Williams, D.; Haynes, K. Differential Virulence of Candida glabrata Glycosylation Mutants. J. Biol. Chem. 2013, 288, 22006–22018. [Google Scholar] [CrossRef][Green Version]
  80. Bates, S.; MacCallum, D.M.; Bertram, G.; Munro, C.A.; Hughes, H.B.; Buurman, E.T.; Brown, A.J.; Odds, F.C.; Gow, N.A. Candida albicans Pmr1p, a Secretory Pathway P-type Ca2+/Mn2+-ATPase, Is Required for Glycosylation and Virulence. J. Biol. Chem. 2005, 280, 23408–23415. [Google Scholar] [CrossRef] [PubMed][Green Version]
  81. Navarro-Arias, M.J.; Defosse, T.A.; Dementhon, K.; Csonka, K.; Mellado-Mojica, E.; Valério, A.D.; González-Hernández, R.J.; Courdavault, V.; Clastre, M.; Hernández, N.V.; et al. Disruption of Protein Mannosylation Affects Candida guilliermondii Cell Wall, Immune Sensing, and Virulence. Front. Microbiol. 2016, 7, 1951. [Google Scholar] [CrossRef] [PubMed]
  82. Pinchai, N.; Juvvadi, P.; Fortwendel, J.R.; Perfect, B.Z.; Rogg, L.E.; Asfaw, Y.G.; Steinbach, W.J. The Aspergillus fumigatus P-Type Golgi Apparatus Ca 2+ /Mn 2+ ATPase PmrA Is Involved in Cation Homeostasis and Cell Wall Integrity but Is Not Essential for Pathogenesis. Eukaryot. Cell 2010, 9, 472–476. [Google Scholar] [CrossRef] [PubMed][Green Version]
  83. Warit, S.; Zhang, N.; Short, A.; Walmsley, R.M.; Oliver, S.G.; Stateva, L.I. Glycosylation deficiency phenotypes resulting from depletion of GDP-mannose pyrophosphorylase in two yeast species. Mol. Microbiol. 2000, 36, 1156–1166. [Google Scholar] [CrossRef][Green Version]
  84. Prill, S.K.-H.; Klinkert, B.; Timpel, C.; Gale, C.A.; Schröppel, K.; Ernst, J.F. PMT family of Candida albicans: Five protein mannosyltransferase isoforms affect growth, morphogenesis and antifungal resistance. Mol. Microbiol. 2004, 55, 546–560. [Google Scholar] [CrossRef] [PubMed]
  85. Timpel, C.; Strahl-Bolsinger, S.; Ziegelbauer, K.; Ernst, J.F. Multiple Functions of Pmt1p-mediated ProteinO-Mannosylation in the Fungal Pathogen Candida albicans. J. Biol. Chem. 1998, 273, 20837–20846. [Google Scholar] [CrossRef][Green Version]
  86. Timpel, C.; Zink, S.; Strahl-Bolsinger, S.; SchröppelK.; Ernst, J. Morphogenesis, Adhesive Properties, and Antifungal Resistance Depend on the Pmt6 Protein Mannosyltransferase in the Fungal Pathogen Candida albicans. J. Bacteriol. 2000, 182, 3063–3071. [Google Scholar] [CrossRef][Green Version]
  87. Mouyna, I.; Kniemeyer, O.; Jank, T.; Loussert, C.; Mellado, E.; Aimanianda, V.; Beauvais, A.; Wartenberg, D.; Sarfati, J.; Bayry, J.; et al. Members of protein O-mannosyltransferase family in Aspergillus fumigatus differentially affect growth, morphogenesis and viability. Mol. Microbiol. 2010, 76, 1205–1221. [Google Scholar] [CrossRef]
  88. Willger, S.D.; Ernst, J.F.; Alspaugh, J.A.; Lengeler, K.B. Characterization of the PMT Gene Family in Cryptococcus neoformans. PLoS ONE 2009, 4, e6321. [Google Scholar] [CrossRef][Green Version]
  89. Rouabhia, M.; Schaller, M.; Corbucci, C.; Vecchiarelli, A.; Prill, S.K.-H.; Giasson, L.; Ernst, J.F. Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases. Infect. Immun. 2005, 73, 4571–4580. [Google Scholar] [CrossRef][Green Version]
  90. Buurman, E.T.; Westwater, C.; Hube, B.; Brown, A.J.P.; Odds, F.C.; Gow, N.A.R. Molecular analysis of CaMnt1p, a mannosyl transferase important for adhesion and virulence of Candida albicans. Proc. Natl. Acad. Sci. USA 1998, 95, 7670–7675. [Google Scholar] [CrossRef][Green Version]
  91. Umemura, M.; Okamoto, M.; Nakayama, K.-I.; Sagane, K.; Tsukahara, K.; Hata, K.; Jigami, Y. GWT1 Gene Is Required for Inositol Acylation of Glycosylphosphatidylinositol Anchors in Yeast. J. Biol. Chem. 2003, 278, 23639–23647. [Google Scholar] [CrossRef][Green Version]
  92. Watanabe, N.-A.; Miyazaki, M.; Horii, T.; Sagane, K.; Tsukahara, K.; Hata, K. E1210, a New Broad-Spectrum Antifungal, Suppresses Candida albicans Hyphal Growth through Inhibition of Glycosylphosphatidylinositol Biosynthesis. Antimicrob. Agents Chemother. 2011, 56, 960–971. [Google Scholar] [CrossRef][Green Version]
  93. Miyazaki, M.; Horii, T.; Hata, K.; Watanabe, N.-A.; Nakamoto, K.; Tanaka, K.; Shirotori, S.; Murai, N.; Inoue, S.; Matsukura, M.; et al. In VitroActivity of E1210, a Novel Antifungal, against Clinically Important Yeasts and Molds. Antimicrob. Agents Chemother. 2011, 55, 4652–4658. [Google Scholar] [CrossRef][Green Version]
  94. Pfaller, M.; Duncanson, F.; Messer, S.A.; Moet, G.J.; Jones, R.N.; Castanheira, M. In Vitro Activity of a Novel Broad-Spectrum Antifungal, E1210, Tested against Aspergillus spp. Determined by CLSI and EUCAST Broth Microdilution Methods. Antimicrob. Agents Chemother. 2011, 55, 5155–5158. [Google Scholar] [CrossRef] [PubMed][Green Version]
  95. Lavie-Richard, M.; Ibata-Ombetta, S.; Dromer, F.; Bordon-Pallier, F.; Jouault, T.; Gaillardin, C. Complete glycosylphosphatidylinositol anchors are required in Candida albicans for full morphogenesis, virulence and resistance to macrophages. Mol. Microbiol. 2002, 44, 841–853. [Google Scholar] [CrossRef] [PubMed][Green Version]
  96. Jain, P.; Garai, P.; Sethi, S.C.; Naqvi, N.; Yadav, B.; Kumar, P.; Singh, S.L.; Yadav, U.; Bhatnagar, S.; Rahul; et al. Modulation of azole sensitivity and filamentation by GPI15, encoding a subunit of the first GPI biosynthetic enzyme, in Candida albicans. Sci. Rep. 2019, 9, 8508. [Google Scholar] [CrossRef] [PubMed]
  97. Li, H.; Zhou, H.; Luo, Y.; Ouyang, H.; Hu, H.; Jin, C. Glycosylphosphatidylinositol (GPI) anchor is required in Aspergillus fumigatus for morphogenesis and virulence. Mol. Microbiol. 2007, 64, 1014–1027. [Google Scholar] [CrossRef]
  98. Schmalhorst, P.S.; Krappmann, S.; Vervecken, W.; Rohde, M.; Müller, M.; Braus, G.H.; Contreras, R.; Braun, A.; Bakker, H.; Routier, F.H. Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus fumigatus. Eukaryot. Cell 2008, 7, 1268–1277. [Google Scholar] [CrossRef][Green Version]
  99. Lamarre, C.; Beau, R.; Balloy, V.; Fontaine, T.; Hoi, J.W.S.; Guadagnini, S.; Berkova, N.; Chignard, M.; Beauvais, A.; Latgé, J.-P. Galactofuranose attenuates cellular adhesion ofAspergillus fumigatus. Cell. Microbiol. 2009, 11, 1612–1623. [Google Scholar] [CrossRef]
  100. Netea, M.G.; Gow, N.A.; Munro, C.A.; Bates, S.; Collins, C.; Ferwerda, G.; Hobson, R.P.; Bertram, G.; Hughes, H.B.; Jansen, T.; et al. Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors. J. Clin. Investig. 2006, 116, 1642–1650. [Google Scholar] [CrossRef]
  101. Cambi, A.; Netea, M.G.; Mora-Montes, H.M.; Gow, N.A.; Hato, S.V.; Lowman, D.W.; Kullberg, B.-J.; Torensma, R.; Williams, D.L.; Figdor, C.G. Dendritic Cell Interaction with Candida albicans Critically Depends on N-Linked Mannan. J. Biol. Chem. 2008, 283, 20590–20599. [Google Scholar] [CrossRef] [PubMed][Green Version]
  102. Yadav, B.; Mora-Montes, H.M.; Wagener, J.; Cunningham, I.; West, L.; Haynes, K.; Brown, A.J.; Gow, N.A. Differences in fungal immune recognition by monocytes and macrophages: N-mannan can be a shield or activator of immune recognition. Cell Surf. 2020, 6, 100042. [Google Scholar] [CrossRef] [PubMed]
  103. Ecker, M.; Mrsa, V.; Hagen, I.; Deutzmann, R.; Strahl, S.; Tanner, W. O-Mannosylation precedes and potentially controls the N -glycosylation of a yeast cell wall glycoprotein. EMBO Rep. 2003, 4, 628–632. [Google Scholar] [CrossRef] [PubMed][Green Version]
  104. McKenzie, C.G.J.; Koser, U.; Lewis, L.E.; Bain, J.M.; Mora-Montes, H.M.; Barker, R.N.; Gow, N.; Erwig, L.P. Contribution of Candida albicans Cell Wall Components to Recognition by and Escape from Murine Macrophages. Infect. Immun. 2010, 78, 1650–1658. [Google Scholar] [CrossRef][Green Version]
  105. Harris, M.; Mora-Montes, H.M.; Gow, N.; Coote, P.J. Loss of mannosylphosphate from Candida albicans cell wall proteins results in enhanced resistance to the inhibitory effect of a cationic antimicrobial peptide via reduced peptide binding to the cell surface. Microbiology 2009, 155, 1058–1070. [Google Scholar] [CrossRef][Green Version]
  106. Juchimiuk, M.; Orłowski, J.; Gawarecka, K.; Świeżewska, E.; Ernst, J.F.; Palamarczyk, G. Candida albicans cis-prenyltransferase Rer2 is required for protein glycosylation, cell wall integrity and hypha formation. Fungal Genet. Biol. 2014, 69, 1–12. [Google Scholar] [CrossRef]
  107. Janik, A.; Niewiadomska, M.; Perlińska-Lenart, U.; Lenart, J.; Kołakowski, D.; Skorupińska-Tudek, K.; Swiezewska, E.; Kruszewska, J.S.; Palamarczyk, G. Inhibition of Dephosphorylation of Dolichyl Diphosphate Alters the Synthesis of Dolichol and Hinders Protein N-Glycosylation and Morphological Transitions in Candida albicans. Int. J. Mol. Sci. 2019, 20, 5067. [Google Scholar] [CrossRef][Green Version]
  108. Zhou, H.; Hu, H.; Zhang, L.; Li, R.; Ouyang, H.; Ming, J.; Jin, C. O-Mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) Is Crucial for Cell Wall Integrity and Conidium Morphology, Especially at an Elevated Temperature. Eukaryot. Cell 2007, 6, 2260–2268. [Google Scholar] [CrossRef][Green Version]
  109. Olson, G.M.; Fox, D.S.; Wang, P.; Alspaugh, J.A.; Buchanan, K.L. Role of Protein O-Mannosyltransferase Pmt4 in the Morphogenesis and Virulence of Cryptococcus neoformans. Eukaryot. Cell 2007, 6, 222–234. [Google Scholar] [CrossRef][Green Version]
  110. Wagener, J.; Echtenacher, B.; Rohde, M.; Kotz, A.; Krappmann, S.; Heesemann, J.; Ebel, F. The Putative α-1,2-Mannosyltransferase AfMnt1 of the Opportunistic Fungal Pathogen Aspergillus fumigatus Is Required for Cell Wall Stability and Full Virulence. Eukaryot. Cell 2008, 7, 1661–1673. [Google Scholar] [CrossRef][Green Version]
  111. Mutz, M.; Roemer, T. The GPI anchor pathway: A promising antifungal target? Futur. Med. Chem. 2016, 8, 1387–1391. [Google Scholar] [CrossRef][Green Version]
  112. Tsukahara, K.; Hata, K.; Nakamoto, K.; Sagane, K.; Watanabe, N.-A.; Kuromitsu, J.; Kai, J.; Tsuchiya, M.; Ohba, F.; Jigami, Y.; et al. Medicinal genetics approach towards identifying the molecular target of a novel inhibitor of fungal cell wall assembly. Mol. Microbiol. 2003, 48, 1029–1042. [Google Scholar] [CrossRef]
  113. Hong, Y.; Maeda, Y.; Watanabe, R.; Ohishi, K.; Mishkind, M.; Riezman, H.; Kinoshita, T. Pig-n, a Mammalian Homologue of Yeast Mcd4p, Is Involved in Transferring Phosphoethanolamine to the First Mannose of the Glycosylphosphatidylinositol. J. Biol. Chem. 1999, 274, 35099–35106. [Google Scholar] [CrossRef][Green Version]
  114. Benachour, A.; Sipos, G.; Flury, I.; Reggiori, F.; Canivenc-Gansel, E.; Vionnet, C.; Conzelmann, A.; Benghezal, M. Deletion of GPI7, a Yeast Gene Required for Addition of a Side Chain to the Glycosylphosphatidylinositol (GPI) Core Structure, Affects GPI Protein Transport, Remodeling, and Cell Wall Integrity. J. Biol. Chem. 1999, 274, 15251–15261. [Google Scholar] [CrossRef][Green Version]
  115. Ouyang, H.; Du, T.; Zhou, H.; Wilson, I.B.H.; Yang, J.; Latgé, J.-P.; Jin, C. Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth. Sci. Rep. 2019, 9, 5857. [Google Scholar] [CrossRef]
  116. Victoria, G.S.; Kumar, P.; Komath, S.S. The Candida albicans homologue of PIG-P, CaGpi19p: Gene dosage and role in growth and filamentation. Microbiology 2010, 156, 3041–3051. [Google Scholar] [CrossRef]
  117. Victoria, G.S.; Yadav, B.; Hauhnar, L.; Jain, P.; Bhatnagar, S.; Komath, S.S. Mutual co-regulation between GPI-N-acetylglucosaminyltransferase and ergosterol biosynthesis in Candida albicans. Biochem. J. 2012, 443, 619–625. [Google Scholar] [CrossRef]
  118. Yadav, B.; Bhatnagar, S.; Ahmad, M.F.; Jain, P.; Pratyusha, V.A.; Kumar, P.; Komath, S.S. First Step of Glycosylphosphatidylinositol (GPI) Biosynthesis Cross-talks with Ergosterol Biosynthesis and Ras Signaling in Candida albicans. J. Biol. Chem. 2014, 289, 3365–3382. [Google Scholar] [CrossRef] [PubMed][Green Version]
  119. Yan, J.; Du, T.; Zhao, W.; Hartmann, T.; Lu, H.; Lü, Y.; Ouyang, H.; Jiang, X.; Sun, L.; Jin, C. Transcriptome and Biochemical Analysis Reveals That Suppression of GPI-Anchor Synthesis Leads to Autophagy and Possible Necroptosis in Aspergillus fumigatus. PLoS ONE 2013, 8, e59013. [Google Scholar] [CrossRef] [PubMed][Green Version]
  120. Lian, T.; Simmer, M.I.; D’Souza, C.A.; Steen, B.R.; Zuyderduyn, S.D.; Jones, S.; Marra, M.A.; Kronstad, J.W. Iron-regulated transcription and capsule formation in the fungal pathogen Cryptococcus neoformans. Mol. Microbiol. 2004, 55, 1452–1472. [Google Scholar] [CrossRef] [PubMed]
  121. Sheppard, D.C. Molecular mechanism of Aspergillus fumigatus adherence to host constituents. Curr. Opin. Microbiol. 2011, 14, 375–379. [Google Scholar] [CrossRef] [PubMed][Green Version]
  122. Calugi, C.; Trabocchi, A.; Guarna, A. Novel small molecules for the treatment of infections caused by Candida albicans: A patent review (2002–2010). Expert Opin. Ther. Pat. 2011, 21, 381–397. [Google Scholar] [CrossRef] [PubMed]
  123. Pérez-García, L.A.; Martínez-Duncker, I.; Montes, H.M.M. The Endoplasmic Reticulum Alpha-Glycosidases as Potential Targets for Virus Control. Curr. Protein Pept. Sci. 2017, 18, 1090–1097. [Google Scholar] [CrossRef]
  124. Orchard, M.G.; Neuss, J.C.; Galley, C.M.; Carr, A.; Porter, D.W.; Smith, P.; Scopes, D.I.; Haydon, D.; Vousden, K.; Stubberfield, C.R.; et al. Rhodanine-3-acetic acid derivatives as inhibitors of fungal protein mannosyl transferase 1 (PMT1). Bioorganic Med. Chem. Lett. 2004, 14, 3975–3978. [Google Scholar] [CrossRef]
  125. Bai, L.; Kovach, A.; You, Q.; Kenny, A.; Li, H. Structure of the eukaryotic protein O-mannosyltransferase Pmt1−Pmt2 complex. Nat. Struct. Mol. Biol. 2019, 26, 704–711. [Google Scholar] [CrossRef]
  126. Salci, T.P.; Negri, M.; Abadio, A.K.R.; Bonfim-Mendonça, P.; Capoci, I.; Caparroz-Assef, S.M.; Donatti, L.; Felipe, M.S.S.; Kioshima, E.S.; Svidzinski, T.I. A new small-molecule KRE2 inhibitor against invasive Candida parapsilosis infection. Futur. Microbiol. 2017, 12, 1283–1295. [Google Scholar] [CrossRef]
  127. Singh, V.; Praveen, V.; Tripathi, D.; Haque, S.; Somvanshi, P.; Katti, S.B.; Tripathi, C.K.M. Isolation, characterization and antifungal docking studies of wortmannin isolated from Penicillium radicum. Sci. Rep. 2015, 5, 11948. [Google Scholar] [CrossRef]
  128. Sawada, Y.; Nishio, M.; Yamamoto, H.; Hatori, M.; Miyaki, T.; Konishi, M.; Oki, T. New antifungal antibiotics, pradimicins D and E. Glycine analogs of pradimicins A and C. J. Antibiot. 1990, 43, 771–777. [Google Scholar] [CrossRef][Green Version]
  129. Takeuchi, T.; Hara, T.; Naganawa, H.; Okada, M.; Hamada, M.; Umezawat, H.; Gomi, S.; Sezaki, M.; Kondo, S. New antifungal antibiotics, benanomicins A and B from an Actinomycete. J. Antibiot. 1988, 41, 807–811. [Google Scholar] [CrossRef]
  130. Ueki, T.; Numata, K.-I.; Ada, Y.S.; Nakajima, T.; Fukagawa, Y.; Oki, T. Studies on the mode of antifungal action of pradimicin antibiotics. I.Lectin-mimic binding of BMY-28864 to yeast mannan in the presence of calcium. J. Antibiot. 1993, 46, 149–161. [Google Scholar] [CrossRef] [PubMed][Green Version]
  131. Watanabe, M.; Tohyama, H.; Hiratani, T.; Watabe, H.; Inoue, S.; Kondo, S.-I.; Takeuchi, T.; Yamaguchi, H. Mode of antifungal action of benanomicin A in Saccharomyces cerevisiae. J. Antibiot. 1997, 50, 1042–1051. [Google Scholar] [CrossRef][Green Version]
  132. Hiramoto, F.; Nomura, N.; Furumai, T.; Oki, T.; Igarashi, Y. Apoptosis-like cell death of Saccharomyces cerevisiae induced by a mannose-binding antifungal antibiotic, pradimicin. J. Antibiot. 2003, 56, 768–772. [Google Scholar] [CrossRef][Green Version]
  133. Oki, T.; Tenmyo, O.; Hirano, M.; Tomatsu, K.; Kamei, H. Pradimicins A, B and C: New antifungal antibiotics. II. In vitro and in vivo biological activities. J. Antibiot. 1990, 43, 763–770. [Google Scholar] [CrossRef] [PubMed][Green Version]
  134. Yasuoka, A.; Oka, S.; Komuro, K.; Shimizu, H.; Kitada, K.; Nakamura, Y.; Shibahara, S.; Takeuchi, T.; Kondo, S.; Shimada, K. Successful treatment of Pneumocystis carinii Pneumonia in mice with benanomicin A (ME1451). Antimicrob. Agents Chemother. 1995, 39, 720–724. [Google Scholar] [CrossRef] [PubMed][Green Version]
  135. Regente, M.; Taveira, G.B.; Pinedo, M.; Elizalde, M.M.; Ticchi, A.J.; Diz, M.S.S.; Carvalho, A.O.; De La Canal, L.; Gomes, V.M. A Sunflower Lectin with Antifungal Properties and Putative Medical Mycology Applications. Curr. Microbiol. 2014, 69, 88–95. [Google Scholar] [CrossRef] [PubMed]
  136. Del Rio, M.; de la Canal, L.; Pinedo, M.; Mora-Montes, H.M.; Regente, M. Effects of the binding of a Helianthus annuus lectin to Candida albicans cell wall on biofilm development and adhesion to host cells. Phytomedicine 2019, 58, 152875. [Google Scholar] [CrossRef] [PubMed]
  137. Shaw, K.J.; Schell, W.A.; Covel, J.; Duboc, G.; Giamberardino, C.; Kapoor, M.; Moloney, M.; Soltow, Q.A.; Tenor, J.L.; Toffaletti, D.L.; et al. In Vitro and In Vivo Evaluation of APX001A/APX001 and Other Gwt1 Inhibitors against Cryptococcus. Antimicrob. Agents Chemother. 2018, 62, e00523-18. [Google Scholar] [CrossRef][Green Version]
  138. Fernandes, C.M.; Dasilva, D.; Haranahalli, K.; McCarthy, J.B.; Mallamo, J.; Ojima, I.; Del Poeta, M. The Future of Antifungal Drug Therapy: Novel Compounds and Targets. Antimicrob. Agents Chemother. 2020, 65, 01719–01720. [Google Scholar] [CrossRef]
  139. Rauseo, A.; Coler-Reilly, A.; Larson, L.; Spec, A. Hope on the Horizon: Novel Fungal Treatments in Development. Open Forum Infect. Dis. 2020, 7, ofaa016. [Google Scholar] [CrossRef][Green Version]
  140. Rodrigues, C.F.; Boas, D.V.; Haynes, K.; Henriques, M. The MNN2 Gene Knockout Modulates the Antifungal Resistance of Biofilms of Candida glabrata. Biomolecules 2018, 8, 130. [Google Scholar] [CrossRef][Green Version]
  141. Juchimiuk, M.; Pasikowska, M.; Zatorska, E.; Laudy, A.E.; Smoleńska-Sym, G.; Palamarczyk, G. Defect in dolichol-dependent glycosylation increases sensitivity of Saccharomyces cerevisiae towards anti-fungal drugs. Yeast 2010, 27, 637–645. [Google Scholar] [CrossRef]
  142. Harries, E.; Carmona, L.; Muñoz, A.; Ibeas, J.I.; Read, N.D.; Gandía, M.; Marcos, J.F. Genes involved in protein glycosylation determine the activity and cell internalization of the antifungal peptide PAF26 in Saccharomyces cerevisiae. Fungal Genet. Biol. 2013, 58-59, 105–115. [Google Scholar] [CrossRef]
  143. Zeng, G.; Xu, X.; Gao, J.; Dantas, A.D.S.; Gow, N.A.; Wang, Y. Inactivating the mannose-ethanolamine phosphotransferase Gpi7 confers caspofungin resistance in the human fungal pathogen Candida albicans. Cell Surf. 2021, 7, 100057. [Google Scholar] [CrossRef] [PubMed]
  144. Chaturvedi, A.K.; Weintraub, S.T.; Lopez-Ribot, J.; Wormley, F.L., Jr. Identification and characterization of Cryptococcus neoformans protein fractions that induce protective immune responses. Proteomics 2013, 13, 3429–3441. [Google Scholar] [CrossRef] [PubMed][Green Version]
  145. Levitz, S.M.; Huang, H.; Ostroff, G.R.; Specht, C.A. Exploiting fungal cell wall components in vaccines. Semin. Immunopathol. 2015, 37, 199–207. [Google Scholar] [CrossRef] [PubMed]
  146. Liao, J.; Pan, B.; Liao, G.; Zhao, Q.; Gao, Y.; Chai, X.; Zhuo, X.; Wu, Q.; Jiao, B.; Pan, W.; et al. Synthesis and immunological studies of β-1,2-mannan-peptide conjugates as antifungal vaccines. Eur. J. Med. Chem. 2019, 173, 250–260. [Google Scholar] [CrossRef] [PubMed]
  147. Bundle, D.R.; Paszkiewicz, E.; Elsaidi, H.R.H.; Mandal, S.S.; Sarkar, S. A Three Component Synthetic Vaccine Containing a β-Mannan T-Cell Peptide Epitope and a β-Glucan Dendritic Cell Ligand. Molecules 2018, 23, 1961. [Google Scholar] [CrossRef][Green Version]
  148. Paulovičová, L.; Paulovičová, E.; Karelin, A.A.; Tsvetkov, Y.E.; Nifantiev, N.E.; Bystrický, S. Humoral and cell-mediated immunity following vaccination with synthetic Candida cell wall mannan derived heptamannoside–protein conjugate. Int. Immunopharmacol. 2012, 14, 179–187. [Google Scholar] [CrossRef] [PubMed]
  149. Liu, M.; Machová, E.; Neščáková, Z.; Medovarská, I.; Clemons, K.V.; Martinez, M.; Chen, V.; Bystrický, S.; Stevens, D.A. Vaccination with mannan protects mice against systemic aspergillosis. Med. Mycol. 2012, 50, 818–828. [Google Scholar] [CrossRef] [PubMed]
  150. Bundle, D.R.; Nycholat, C.; Costello, C.; Rennie, R.; Lipinski, T. Design of a Candida albicans Disaccharide Conjugate Vaccine by Reverse Engineering a Protective Monoclonal Antibody. ACS Chem. Biol. 2012, 7, 1754–1763. [Google Scholar] [CrossRef]
  151. Han, Y.; Rhew, K.Y. Comparison of two Candida mannan vaccines: The role of complement in protection against disseminated candidiasis. Arch. Pharmacal Res. 2012, 35, 2021–2027. [Google Scholar] [CrossRef] [PubMed]
  152. Rosario-Colon, J.; Eberle, K.; Adams, A.; Courville, E.; Xin, H. Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection. Int. J. Mol. Sci. 2021, 22, 6162. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Schematic diagram representing the Candida albicans N-linked and O-linked glycans. (A) N-linked glycan. The outer chain elaboration begins with the addition of an α-1,6-mannose residue to the N-linked glycan core by the α-1,6-mannosyltransferase Och1. Then, an α-1,6-mannose backbone is synthesized by M-Pol I and M-Pol II complexes, and this is branched with α-1,2-mannooligomers that can be decorated with phosphomannan, β-1,2-mannose, or α-1,3-mannose units. (B) O-linked glycan. The addition of the first mannose residue is initiated in the endoplasmic reticulum by protein mannosyltransferases encoded by the PMT gene family. The glycoprotein is then transported to the Golgi complex for the addition of further mannose residues by mannosyltransferases Mnt1 and Mnt2.
Figure 1. Schematic diagram representing the Candida albicans N-linked and O-linked glycans. (A) N-linked glycan. The outer chain elaboration begins with the addition of an α-1,6-mannose residue to the N-linked glycan core by the α-1,6-mannosyltransferase Och1. Then, an α-1,6-mannose backbone is synthesized by M-Pol I and M-Pol II complexes, and this is branched with α-1,2-mannooligomers that can be decorated with phosphomannan, β-1,2-mannose, or α-1,3-mannose units. (B) O-linked glycan. The addition of the first mannose residue is initiated in the endoplasmic reticulum by protein mannosyltransferases encoded by the PMT gene family. The glycoprotein is then transported to the Golgi complex for the addition of further mannose residues by mannosyltransferases Mnt1 and Mnt2.
Jof 07 00875 g001
Figure 2. Schematic diagram representing glycosylphosphatidylinositol (GPI) anchor synthesis. GPIs are composed of an inositol molecule that is modified in the endoplasmic reticulum. The Smp3 protein is responsible for adding the fourth mannose residue, which is important for protein transfer.
Figure 2. Schematic diagram representing glycosylphosphatidylinositol (GPI) anchor synthesis. GPIs are composed of an inositol molecule that is modified in the endoplasmic reticulum. The Smp3 protein is responsible for adding the fourth mannose residue, which is important for protein transfer.
Jof 07 00875 g002
Table 1. Phenotype of mutant strains in genes involved in N-linked or O-linked glycosylation, glycosylphosphatidylinositol anchor synthesis, and non-conventional glycosylation processes, in medically relevant fungal species.
Table 1. Phenotype of mutant strains in genes involved in N-linked or O-linked glycosylation, glycosylphosphatidylinositol anchor synthesis, and non-conventional glycosylation processes, in medically relevant fungal species.
GeneMutant PhenotypeImpact on VirulenceReferences
N-Linked Glycosylation
CWH41Candida albicans
Cellular aggregates, defects in cell wall composition.

Aspergillus fumigatus
Reduction in conidia formation, changes in the cell wall composition, polar growth, and hyphal elongation.
Attenuated virulence in a murine model.

None found.
[26,61]
ROT2C. albicans
Cellular aggregates, defects in cell wall composition.

Candida glabrata
None found.
 
Attenuated virulence in a murine model.

Decreased virulence in Drosophila melanogaster.
[26,62]
MNS1C. albicans
Few phenotypical changes.

C. glabrata
None found.
 
Virulence affected.

Decreased virulence in a murine model of systemic candidiasis.
[26,62,63]
OCH1C. albicans
Defects in the cell wall, cellular aggregates, decreased growth rate, and reduced ability to stimulate cytokine production.

Candida parapsilosis
Changes in cell morphology and cell wall composition, susceptibility to cell wall perturbing agents, and reduced ability to stimulate cytokine production.

Sporothrix schenckii
Minimal changes in the phenotype and the cell wall.

A. fumigatus:
Defects in ability to sporulate, non-significant changes in phenotype.

Cryptococcus neoformans
None found.

Attenuated virulence in a murine model.

Attenuated virulence in neonate and adult murine models.

Attenuated virulence in a murine model and the invertebrates Galleria mellonella and Tenebrio molitor.

None found.

Slightly attenuated virulence.
[26,64,65,66,67,68,69,70]
ANP1C. glabrata
None found.
Decreased virulence in both murine model of systemic candidiasis and D. melanogaster. [62]
MNN14C. albicans
Defects in cell wall composition, but normal ability to stimulate cytokine production.
Virulence attenuation in a murine model of disseminated candidiasis.[71]
MNN2C. albicans
Changes in phenotype.
Attenuated virulence in G. mellonella and a murine model.[72]
MNN4C. albicans
Changes in the C. albicans–macrophage interaction.

Candida tropicalis
Changes in the yeast–macrophage interaction.

C. glabrata
None found.
Attenuated virulence in the G. mellonella model but not in a murine model.

Attenuated virulence in G. mellonella model.

Attenuated virulence in a murine model of systemic candidiasis.
[30,62,73,74]
MNN5C. albicans
Hypersensitivity to wall perturbing agents, inability to undergo dimorphism.
 
Attenuated virulence in a murine model.
[75]
MNN9C. albicans
Changes in the cell wall, osmotic instability, defective hyphae, and cell agglutination.

A. fumigatus
Defects in the cell wall, changes in morphogenesis, sensitivity to wall perturbing agents.
 
None found.

None found.
[76,77]
MNN10C. albicans
Abnormal organization of the cell wall.

C. glabrata
Abnormal growth in vitro
Decreased virulence.

Decreased virulence in both murine model of systemic candidiasis and D. melanogaster
[62,78]
MNN11C. glabrata
None found.
 
Virulence is not affected.
[79]
KRE2/MNT1C. albicans
Cellular aggregates, susceptibility to cell wall perturbing agents, reduction in the ability to stimulate cytokine production.

C. glabrata
None found.
 
Attenuated virulence in a murine model.

Virulence attenuation in both a murine model of candidiasis and D. melanogaster.
[31,62]
KTR3C. glabrata
None found.
Virulence attenuation in D. melanogaster.[62]
PMR1C. albicans
Cellular aggregates, defects in the cell wall composition, hypersensitivity to wall perturbing agents.

Candida guilliermondii
Affected cell growth, morphology, cell wall, biofilm formation, and interaction with PBMC.

A. fumigatus
Defects in growth, changes in the cell wall composition, increased content of β-glucan and chitin. Hypersensitivity to cell wall inhibitors.
 
Attenuated virulence in a murine model.

Attenuated virulence in a murine model and G. mellonella.

None found.
[80,81,82]
ALG6C. glabrata
None found.
Virulence attenuation in D. melanogaster.[62]
ALG13C. albicans
Defects in the hypha and biofilm formation, changes in the cell wall carbohydrate content. Defects in N-linked glycosylation.
 
None found.
[23]
DPM1C. albicans
Susceptibility to wall perturbing agents, altered cell wall composition and affected growth, increased chitin levels.
 
None found.
[21]
DPM2C. albicans
Susceptibility to wall perturbing agents and changes in the cell wall integrity.
 
None found.
[21]
DPM3C. albicans
Growth rate attenuation and increased chitin levels.
 
None found.
[21]
SRB1C. albicans
Changes in morphology, growth, sensitivity to antifungal agents and cell wall inhibitors, and glycosylation defects.
 
None found.
[83]
O-Linked Glycosylation
PMT1C. albicans
Hypersensitivity to antifungal agents and changes in the cell wall components.

C. glabrata
None found.

A. fumigatus
Growth defects, changes in the cell wall, defects in cell morphology, and conidia formation.

C. neoformans:
None found.
 
Severely affected virulence in a murine model.

Virulence attenuation in D. melanogaster.

None found.

Attenuated virulence in a macrophage death model and a murine model.
[62,84,85,86,87,88]
PMT3C. glabrata
None found.
Decreased virulence in a murine model of systemic candidiasis.[62]
PMT4C. albicans
Hypersensitivity to antifungal agents and changes in the cell wall components.

C. glabrata
None found.

A. fumigatus
Growth defects, changes in the cell wall, defects in cell morphology, and conidia formation.

C. neoformans:
Morphological defects, alterations in the cell wall.
 
Severely affected virulence in a murine model.

Decreased virulence in a murine model of systemic candidiasis.

None found.

Virulence affected.
[62,84,86,87,88,89]
PMT5C. albicans:
Defects in morphogenesis and susceptibility to antifungal agents.
 
None found.
[84]
PMT6C. albicans:
None found.
 
Severely affected virulence in a murine model.
[85,86]
MNT1C. albicans:
Changes in cell morphology, inability to adhere to human buccal epithelial cells, hypersensitivity to wall perturbing agents.

A. fumigatus:
Reduction in the cell wall thickness, decreased cell growth.
 
Attenuated virulence in a murine model.

Attenuated virulence.
[34,90]
MNT2C. albicans:
Few defects in phenotype.
 
Attenuated virulence.
[34]
Glycosylphosphatidylinositol Anchors
GWT1Candida spp., Aspergillus spp., Fusarium spp., and Scedosporium spp
Changes in the cell wall integrity, structure, growth, and dimorphism.

C. albicans:
Growth and morphological changes, reduced adherence, growth, and dimorphism.
 
Virulence affected.

None found.
[6,91,92,93,94]
GPI7C. albicans
Effects on chlamydospore formation, altered levels of plasma membrane GPI proteins, abnormal polarity, and growth.
 
None found.
[95]
GPI15C. albicans
Presents hypofilamentous, ergosterol defective, and azole sensitive.
 
Virulence affected, less virulence in vivo.
[96]
GPI3A. fumigatus
Growth rate significantly slower, sensitivity to SDS, increased levels of the chitinase ChiB.
 
Reduced virulence in a murine model.
[97]
Non-Conventional Glycosylation Processes
glfBA. fumigatus
Poor growth.
 
Attenuated virulence in a murine model.
[45,98]
umg1A. fumigatus
Thinner cell wall, susceptibility to antifungal agents, and hyper-adherent cells.
 
None found.
[99]
ktr4 and ktr7A. fumigatus
Severe phenotype defects, showing hyperbranched mycelium, loss of polarity during conidia germination, and hyphal elongation.
 
Virulence affected.
[46]
KTR3C. neoformans
Sensitivity to SDS and NaCl.
 
Attenuated virulence in a murine model.
[54]
RmlDS. schenckii
Few phenotypical changes, alterations in the cell wall composition and organization.
 
Attenuated virulence in the G. mellonella model.
[57]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Gómez-Gaviria, M.; Vargas-Macías, A.P.; García-Carnero, L.C.; Martínez-Duncker, I.; Mora-Montes, H.M. Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host. J. Fungi 2021, 7, 875. https://doi.org/10.3390/jof7100875

AMA Style

Gómez-Gaviria M, Vargas-Macías AP, García-Carnero LC, Martínez-Duncker I, Mora-Montes HM. Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host. Journal of Fungi. 2021; 7(10):875. https://doi.org/10.3390/jof7100875

Chicago/Turabian Style

Gómez-Gaviria, Manuela, Ana P. Vargas-Macías, Laura C. García-Carnero, Iván Martínez-Duncker, and Héctor M. Mora-Montes. 2021. "Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host" Journal of Fungi 7, no. 10: 875. https://doi.org/10.3390/jof7100875

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop