Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Plasmids, and Growth Media
2.2. Urinary Tract Infection in Mice
2.3. Enzyme-Linked Immunosorbent Assay (ELISA)
2.4. In Vitro Luminescence Measurement
2.5. In Vivo Bioluminescence and Antifungal Treatment
3. Results
3.1. Fate of C. glabrata during Urinary Tract Infections
3.2. Expression of a Codon Adapted Red-Shifted Luciferase from the ENO1-Promoter Results in High Bioluminescence In Vitro
3.3. Expression of a Codon Adapted Red-Shifted Luciferase from the TEF1-Promoter Results in the Highest Luminescence In Vivo
3.4. In Vivo Monitoring of Treatment of a C. glabrata Urinary Tract Infection with Fluconazole
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Schrevens, S.; Sanglard, D. Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging. J. Fungi 2021, 7, 844. https://doi.org/10.3390/jof7100844
Schrevens S, Sanglard D. Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging. Journal of Fungi. 2021; 7(10):844. https://doi.org/10.3390/jof7100844
Chicago/Turabian StyleSchrevens, Sanne, and Dominique Sanglard. 2021. "Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging" Journal of Fungi 7, no. 10: 844. https://doi.org/10.3390/jof7100844