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Article

An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield

1
iAMB—Institute of Applied Microbiology, ABBt—Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
2
Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, 52425 Jülich, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
J. Fungi 2021, 7(1), 20; https://doi.org/10.3390/jof7010020
Received: 22 November 2020 / Revised: 27 December 2020 / Accepted: 29 December 2020 / Published: 31 December 2020
(This article belongs to the Special Issue Smut Fungi)
Ustilago maydis, a member of the Ustilaginaceae family, is a promising host for the production of several metabolites including itaconic acid. This dicarboxylate has great potential as a bio-based building block in the polymer industry, and is of special interest for pharmaceutical applications. Several itaconate overproducing Ustilago strains have been generated by metabolic and morphology engineering. This yielded stabilized unicellular morphology through fuz7 deletion, reduction of by-product formation through deletion of genes responsible for itaconate oxidation and (glyco)lipid production, and the overexpression of the regulator of the itaconate cluster ria1 and the mitochondrial tricarboxylate transporter encoded by mttA from Aspergillusterreus. In this study, itaconate production was further optimized by consolidating these different optimizations into one strain. The combined modifications resulted in itaconic acid production at theoretical maximal yield, which was achieved under biotechnologically relevant fed-batch fermentations with continuous feed. View Full-Text
Keywords: itaconic acid; U. maydis; metabolic engineering; fungi; yeast itaconic acid; U. maydis; metabolic engineering; fungi; yeast
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MDPI and ACS Style

Becker, J.; Hosseinpour Tehrani, H.; Ernst, P.; Blank, L.M.; Wierckx, N. An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield. J. Fungi 2021, 7, 20. https://doi.org/10.3390/jof7010020

AMA Style

Becker J, Hosseinpour Tehrani H, Ernst P, Blank LM, Wierckx N. An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield. Journal of Fungi. 2021; 7(1):20. https://doi.org/10.3390/jof7010020

Chicago/Turabian Style

Becker, Johanna, Hamed Hosseinpour Tehrani, Philipp Ernst, Lars Mathias Blank, and Nick Wierckx. 2021. "An Optimized Ustilago maydis for Itaconic Acid Production at Maximal Theoretical Yield" Journal of Fungi 7, no. 1: 20. https://doi.org/10.3390/jof7010020

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