Functional Defect of Neutrophils Causing Dermatophytosis: Case Report
1
Clinical Immunology, Faculdade de Medicina, Centro Universitario Saude ABC, Andre 09060-870, Brazil
2
Division of Rheumatology, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo 04023-062, Brazil
3
Fleury Laboratories, Sao Paulo 04023-062, Brazil
*
Author to whom correspondence should be addressed.
†
These two authors contributed equally.
J. Fungi 2020, 6(4), 238; https://doi.org/10.3390/jof6040238
Received: 10 September 2020
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Revised: 9 October 2020
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Accepted: 14 October 2020
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Published: 22 October 2020
(This article belongs to the Special Issue Innate Immunity in Fungal Infections)
Background: NADPH-oxidase and myeloperoxidase (MPO) play an important role on defense against pathogenic microorganisms. Defects on these mechanisms have been described in association with recurrent infections due to such as Staphylococcus aureus and Candida albicans. We describe a patient with partial disturbance of intracellular microorganism destruction clinically manifested by recurrent fungal infection. Case report and results: A 58-year-old male rural farmer has suffered with superficial mycosis affecting hands, nails and right ankle persisting for 20 years. He was treated with several antifungal drugs with no improvement. Mycological scraping isolated Trichophyton rubrum. Immunological evaluation showed impaired T cell proliferation to Candidin and impaired neutrophil burst oxidative after specific stimulation with Candida albicans. The patient’s DNA was extracted from peripheral blood leukocytes for whole exome sequencing (WES) analysis. Two heterozygous variants of undetermined significance were screened accordingly: (1) MPO A332V (c.995G>A; rs28730837); and (2) NCF1 G83R (c.247G>A; rs139225348). Conclusions: Functional leukocyte evaluation with heterozygous variants in MPO and NCF1 suggest that these defects were associated with the susceptibility to dermatophytosis in our patient. We have developed a fast, effective and safe trial for screening individuals with yeast infections.
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Keywords:
neutrophil; myeloperoxidase; Trichophyton; fungal infection; NCF1; chronic granulomatous disease
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MDPI and ACS Style
Constantino-Silva, R.N.; Perazzio, S.F.; Weidebach, N.d.A.; Grumach, A.S. Functional Defect of Neutrophils Causing Dermatophytosis: Case Report. J. Fungi 2020, 6, 238. https://doi.org/10.3390/jof6040238
AMA Style
Constantino-Silva RN, Perazzio SF, Weidebach NdA, Grumach AS. Functional Defect of Neutrophils Causing Dermatophytosis: Case Report. Journal of Fungi. 2020; 6(4):238. https://doi.org/10.3390/jof6040238
Chicago/Turabian StyleConstantino-Silva, Rosemeire N., Sandro F. Perazzio, Nicolas d.A. Weidebach, and Anete S. Grumach. 2020. "Functional Defect of Neutrophils Causing Dermatophytosis: Case Report" Journal of Fungi 6, no. 4: 238. https://doi.org/10.3390/jof6040238
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