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Article

Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum

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Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de León, Avenida Portugal 41, 24071 León, Spain
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Grupo Universitario de Investigación en Ingeniería y Agricultura Sostenible (GUIIAS), Área de Microbiología, Escuela de Ingeniería Agraria y Forestal, Universidad de León, Campus de Ponferrada, Avenida Astorga s/n, 24401 Ponferrada, Spain
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Author to whom correspondence should be addressed.
J. Fungi 2020, 6(3), 176; https://doi.org/10.3390/jof6030176
Received: 1 August 2020 / Revised: 24 August 2020 / Accepted: 15 September 2020 / Published: 17 September 2020
(This article belongs to the Special Issue Fungal Endophytes in Agriculture and Ecosystems)
Self-inhibitory processes are a common feature shared by different organisms. One of the main mechanisms involved in these interactions regarding microorganisms is the release of toxic diffusible substances into the environment. These metabolites can exert both antimicrobial effects against other organisms as well as self-inhibitory ones. The in vitro evaluation of these effects against other organisms has been widely used to identify potential biocontrol agents against phytopathogenic microorganisms. In the present study, we performed membrane assays to compare the self-inhibitory effects of soluble metabolites produced by several Trichoderma isolates and their antifungal activity against a phytopathogenic strain of Fusarium oxysporum. The results demonstrated that Trichoderma spp. present a high self-inhibitory activity in vitro, being affected in both their growth rate and the macroscopic structure of their colonies. These effects were highly similar to those exerted against F. oxysporum in the same conditions, showing no significant differences in most cases. Consequently, membrane assays may not be very informative by themselves to assess putative biocontrol capabilities. Therefore, different methods, or a combination of antifungal and self-inhibitory experiments, could be a better approach to evaluate the potential biocontrol activity of microbial strains in order to pre-select them for further in vivo trials. View Full-Text
Keywords: self-inhibition; autotoxicity; autoinhibition; membrane assay; secondary metabolites; diffusible metabolites; antifungal; fungi; Trichoderma; Fusarium self-inhibition; autotoxicity; autoinhibition; membrane assay; secondary metabolites; diffusible metabolites; antifungal; fungi; Trichoderma; Fusarium
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MDPI and ACS Style

Álvarez-García, S.; Mayo-Prieto, S.; Gutiérrez, S.; Casquero, P.A. Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum. J. Fungi 2020, 6, 176. https://doi.org/10.3390/jof6030176

AMA Style

Álvarez-García S, Mayo-Prieto S, Gutiérrez S, Casquero PA. Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum. Journal of Fungi. 2020; 6(3):176. https://doi.org/10.3390/jof6030176

Chicago/Turabian Style

Álvarez-García, Samuel, Sara Mayo-Prieto, Santiago Gutiérrez, and Pedro A. Casquero. 2020. "Self-Inhibitory Activity of Trichoderma Soluble Metabolites and Their Antifungal Effects on Fusarium oxysporum" Journal of Fungi 6, no. 3: 176. https://doi.org/10.3390/jof6030176

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