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The Dynamic Influence of Olorofim (F901318) on the Cell Morphology and Organization of Living Cells of Aspergillus fumigatus
Open AccessCommunication

Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers

1
Department of Biochemistry, Genetics, and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0028, South Africa
2
Division of Forest Diseases and Insect Pests, National Institute of Forest Science, Seoul 02455, Korea
3
Department of Plant and Soil Sciences, FABI, University of Pretoria, Pretoria 0028, South Africa
*
Authors to whom correspondence should be addressed.
J. Fungi 2020, 6(2), 48; https://doi.org/10.3390/jof6020048
Received: 10 March 2020 / Revised: 5 April 2020 / Accepted: 10 April 2020 / Published: 14 April 2020
Species in genera of the fungal family Ceratocystidaceae are known to have different mating strategies, including heterothallism and homothallism. Of these, species of Ceratocystis, typified by the pathogen Ceratocystis fimbriata all undergo unidirectional mating-type switching. This implies that the pathogens possess the ability to self, but also to undergo sexual outcrossing between isolates of different mating types. In this study, we extended the recently developed microsatellite-based technique to determine the extent to which outcrossing occurs in ascospore masses of haploid fungi to two field collections of Ceratocystis albifundus. In this way, the role of reproductive strategies in shaping population structure and diversity could be better understood. Results showed that a high frequency of outcrossing occurs in isolates of the pathogen from both non-native and native areas. This explains the high level of genetic diversity previously observed in this population despite the fact that this pathogen has the ability to self. View Full-Text
Keywords: genotypic diversity; population; reproduction; sex genotypic diversity; population; reproduction; sex
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MDPI and ACS Style

Lee, D.-H.; Wingfield, B.D.; Roux, J.; Wingfield, M.J. Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers. J. Fungi 2020, 6, 48. https://doi.org/10.3390/jof6020048

AMA Style

Lee D-H, Wingfield BD, Roux J, Wingfield MJ. Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers. Journal of Fungi. 2020; 6(2):48. https://doi.org/10.3390/jof6020048

Chicago/Turabian Style

Lee, Dong-Hyeon; Wingfield, Brenda D.; Roux, Jolanda; Wingfield, Michael J. 2020. "Quantification of Outcrossing Events in Haploid Fungi Using Microsatellite Markers" J. Fungi 6, no. 2: 48. https://doi.org/10.3390/jof6020048

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