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J. Fungi 2018, 4(3), 82; https://doi.org/10.3390/jof4030082

Genotypic Diversity Is Independent of Pathogenicity in Colombian Strains of Cryptococcus neoformans and Cryptococcus gattii in Galleria mellonella

1
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá 110931, Colombia
2
Unidad de Investigación en Proteomica y Micosis Humanas, Grupo de Investigacion en Enfermedades Infecciosas, Dpto de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá 110231, Colombia
*
Author to whom correspondence should be addressed.
Received: 8 June 2018 / Revised: 29 June 2018 / Accepted: 30 June 2018 / Published: 5 July 2018
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Abstract

Cryptococcosis is a potentially fatal opportunistic mycosis that affects the lungs and central nervous system. It has been suggested that certain strains of C. neoformans/C. gattii may have the potential to be more virulent according to the molecular type. This study aims to investigate the association between virulence in the G. mellonella model and genotypic diversity of Colombian clinical and environmental isolates of C. neoformans/C. gattii. A total of 33 clinical and 12 environmental isolates were selected according to their geographical origin and sequence types (STs). Pathogenicity was determined using the G. mellonella model, and the cell and capsular size before and after inoculation was determined. For C. neoformans, virulence in G. mellonella revealed that death occurred on average on day 6 (p < 0.05) and that ST5C, 6C, 25C and 71C were the most virulent. In C. gattii, death occurred at 7.3 days (p < 0.05), and ST47C, 58C, 75A and 106C were the most virulent. Capsular size increased for both species after passage in G. mellonella. In conclusion, the pathogenicity of Cryptococcus strains in the G. mellonella invertebrate model is independent of molecular type or pathogenicity factor, even within the same ST, but it is possible to find variable degrees of pathogenicity. View Full-Text
Keywords: Cryptococcus neoformans; Cryptococcus gattii; pathogenicity; Galleria mellonella; multi locus sequence typing (MLST); Colombia Cryptococcus neoformans; Cryptococcus gattii; pathogenicity; Galleria mellonella; multi locus sequence typing (MLST); Colombia
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Velez, N.; Alvarado, M.; Parra-Giraldo, C.M.; Sánchez-Quitian, Z.A.; Escandón, P.; Castañeda, E. Genotypic Diversity Is Independent of Pathogenicity in Colombian Strains of Cryptococcus neoformans and Cryptococcus gattii in Galleria mellonella. J. Fungi 2018, 4, 82.

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