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Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation

1
Institute of Microbiology, University of Innsbruck, Technikerstraße 25d, A-6020 Innsbruck, Austria
2
Institute of Botany, University of Innsbruck, Sternwartestraße 15, A-6020 Innsbruck, Austria
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Francesca Scandellari
J. Fungi 2015, 1(1), 55-75; https://doi.org/10.3390/jof1010055
Received: 6 March 2015 / Revised: 20 April 2015 / Accepted: 23 April 2015 / Published: 30 April 2015
(This article belongs to the Special Issue Mycorrhizal Fungi in Sensitive Environments)
Ectomycorrhizae (EM) are important for the survival of seedlings and trees, but how they will react to global warming or changes in soil fertility is still in question. We tested the effect of soil temperature manipulation and nitrogen fertilization on EM communities in a high-altitude Pinus cembra afforestation. The trees had been inoculated in the 1960s in a nursery with a mixture of Suillus placidus, S. plorans and S. sibircus. Sampling was performed during the third year of temperature manipulation in June and October 2013. Root tips were counted, sorted into morphotypes, and sequenced. Fungal biomass was measured as ergosterol and hyphal length. The EM potential of the soil was assessed with internal transcribed spacers (ITS) clone libraries from in-growth mesh bags (MB). Temperature manipulation of ± 1 °C had no effect on the EM community. A total of 33 operational taxonomic units (OTUs) were identified, 20 from the roots, 13 from MB. The inoculated Suillus spp. colonized 82% of the root tips, thus demonstrating that the inoculation was sustainable. Nitrogen fertilization had no impact on the EM community, but promoted depletion in soil organic matter, and caused a reduction in soil fungal biomass. View Full-Text
Keywords: keystone mycobionts; Suillus placidus; afforestation; sustainable mycorrhiza inoculation keystone mycobionts; Suillus placidus; afforestation; sustainable mycorrhiza inoculation
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Rainer, G.; Kuhnert, R.; Unterholzer, M.; Dresch, P.; Gruber, A.; Peintner, U. Host-Specialist Dominated Ectomycorrhizal Communities of Pinus cembra are not Affected by Temperature Manipulation. J. Fungi 2015, 1, 55-75.

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