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Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

1
Unit of Cardiovascular Research, Minerva Institute for Medical Research, Biomedicum Helsinki, 00290 Helsinki, Finland
2
Department of Surgery, South Karelia Central Hospital, 53130 Lappeenranta, Finland
3
Heart and Lung Center, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland
4
Drug Research Programme, Division of Pharmacology and Pharmacotherapy, University of Helsinki, 00014 Helsinki, Finland
5
Abdominal Center, Nephrology, University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland
6
Clinical Chemistry and Hematology, University of Helsinki and Helsinki University Hospital, 00014 Helsinki, Finland
7
Clinical Neurosciences, Neurology, University of Helsinki and Jorvi Hospital of Helsinki University Hospital, 02740 Espoo, Finland
*
Author to whom correspondence should be addressed.
J. Cardiovasc. Dev. Dis. 2019, 6(2), 16; https://doi.org/10.3390/jcdd6020016
Received: 31 January 2019 / Revised: 30 March 2019 / Accepted: 1 April 2019 / Published: 4 April 2019
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Abstract

The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration. View Full-Text
Keywords: let-7c; miRNAs; epicardial cells; cardiac regeneration; proliferating cardiomyocytes; fibrin let-7c; miRNAs; epicardial cells; cardiac regeneration; proliferating cardiomyocytes; fibrin
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Narumanchi, S.; Kalervo, K.; Perttunen, S.; Wang, H.; Immonen, K.; Kosonen, R.; Laine, M.; Ruskoaho, H.; Tikkanen, I.; Lakkisto, P.; Paavola, J. Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish. J. Cardiovasc. Dev. Dis. 2019, 6, 16.

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J. Cardiovasc. Dev. Dis. EISSN 2308-3425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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