Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
AbstractThe let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration. View Full-Text
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Narumanchi, S.; Kalervo, K.; Perttunen, S.; Wang, H.; Immonen, K.; Kosonen, R.; Laine, M.; Ruskoaho, H.; Tikkanen, I.; Lakkisto, P.; Paavola, J. Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish. J. Cardiovasc. Dev. Dis. 2019, 6, 16.
Narumanchi S, Kalervo K, Perttunen S, Wang H, Immonen K, Kosonen R, Laine M, Ruskoaho H, Tikkanen I, Lakkisto P, Paavola J. Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish. Journal of Cardiovascular Development and Disease. 2019; 6(2):16.Chicago/Turabian Style
Narumanchi, Suneeta; Kalervo, Karri; Perttunen, Sanni; Wang, Hong; Immonen, Katariina; Kosonen, Riikka; Laine, Mika; Ruskoaho, Heikki; Tikkanen, Ilkka; Lakkisto, Päivi; Paavola, Jere. 2019. "Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish." J. Cardiovasc. Dev. Dis. 6, no. 2: 16.
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