A PMAxxTM qPCR Assay Reveals That Dietary Administration of the Microalgae Tetraselmis chuii Does Not Affect Salmonella Infantis Caecal Content in Early-Treated Broiler Chickens
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Microalgae Biomass
2.2. Bacterial Preparation
2.3. Determination of S. Infantis Load
2.3.1. Cultivation of S. Infantis and Extraction of Genomic DNA
2.3.2. Amplification of the invA Gene
2.3.3. Staining of Viable and Non-Viable Salmonella Cells Using PMAxxTM
2.4. Experimental Setup
2.5. Statistical Analyses
3. Results
3.1. Amplification of the invA Gene
3.2. Inhibitory Effects of PMAxxTM
3.3. Effects of Microalgae Administration on S. Infantis Caecal Load
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Dilution | Ct1x | Ct2x | Ct3x | Average Ct | SD | CV (%) |
---|---|---|---|---|---|---|
−1 | 16.93 | 16.94 | 17.07 | 16.98 | 0.06 | 0.38 |
−2 | 20.19 | 20.49 | 20.88 | 20.52 | 0.28 | 1.38 |
−3 | 23.97 | 24.26 | 24.25 | 24.16 | 0.13 | 1.54 |
−4 | 28.83 | 28.81 | 28.88 | 28.84 | 0.03 | 0.10 |
−5 | 32.56 | 32.46 | 32.62 | 32.55 | 0.07 | 0.21 |
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Corrales-Martinez, J.; Ortega-Paredes, D.; Šefcová, M.A.; Larrea-Álvarez, C.M.; de Janon, S.; Medina-Santana, J.; Molina-Cuasapaz, G.; Vinueza-Burgos, C.; Revajová, V.; Larrea-Álvarez, M.; et al. A PMAxxTM qPCR Assay Reveals That Dietary Administration of the Microalgae Tetraselmis chuii Does Not Affect Salmonella Infantis Caecal Content in Early-Treated Broiler Chickens. Vet. Sci. 2022, 9, 487. https://doi.org/10.3390/vetsci9090487
Corrales-Martinez J, Ortega-Paredes D, Šefcová MA, Larrea-Álvarez CM, de Janon S, Medina-Santana J, Molina-Cuasapaz G, Vinueza-Burgos C, Revajová V, Larrea-Álvarez M, et al. A PMAxxTM qPCR Assay Reveals That Dietary Administration of the Microalgae Tetraselmis chuii Does Not Affect Salmonella Infantis Caecal Content in Early-Treated Broiler Chickens. Veterinary Sciences. 2022; 9(9):487. https://doi.org/10.3390/vetsci9090487
Chicago/Turabian StyleCorrales-Martinez, Joselyn, David Ortega-Paredes, Miroslava Anna Šefcová, César Marcelo Larrea-Álvarez, Sofía de Janon, José Medina-Santana, Gabriel Molina-Cuasapaz, Christian Vinueza-Burgos, Viera Revajová, Marco Larrea-Álvarez, and et al. 2022. "A PMAxxTM qPCR Assay Reveals That Dietary Administration of the Microalgae Tetraselmis chuii Does Not Affect Salmonella Infantis Caecal Content in Early-Treated Broiler Chickens" Veterinary Sciences 9, no. 9: 487. https://doi.org/10.3390/vetsci9090487