The Protective Efficacy of an Inactivated Vaccine against Avibacterium paragallinarum Field Isolates
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Bacteria and Media
2.2. Vaccine
2.3. Immunization Procedures
2.4. Antibody Titers Detected by Hemagglutination Inhibition Test
2.5. Challenge Infection Experiments
2.6. Clinical Scoring and Necropsy
2.7. Av. paragallinarum Quantification by Quantitative Real-Time PCR
2.8. Statistical Analysis
3. Results
3.1. Antibody Titers Detected by Hemagglutination Inhibition Test
3.2. Clinical Sign Score
3.3. Body Weight
3.4. Egg Production
3.5. Pathological Changes of Infraorbital Sinus and Trachea
3.6. Bacterial Shedding Quantification
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups | Number of Chickens | Times of Vaccination | Age of Vaccination | Strain for Challenge | Age of Challenge |
---|---|---|---|---|---|
A1 | 10 | 1 | 110 d | 2019−HB65 | 130 d |
B1 | 10 | 1 | 110 d | 2019−HB68 | 130 d |
C1 | 10 | 1 | 110 d | 2020−JS80 | 130 d |
A2 | 10 | 2 | 53 d and 110 d | 2019−HB65 | 130 d |
B2 | 10 | 2 | 53 d and 110 d | 2019−HB68 | 130 d |
C2 | 10 | 2 | 53 d and 110 d | 2020−JS80 | 130 d |
A0 | 10 | / | / | 2019−HB65 | 130 d |
B0 | 10 | / | / | 2019−HB68 | 130 d |
C0 | 10 | / | / | 2020−JS80 | 130 d |
D | 10 | / | / | / | / |
Groups | 7 dpc | 14 dpc |
---|---|---|
A1 | 15.71 ± 12.94 a | 20.00 ± 15.12 b |
A2 | 28.57 ± 15.52 | 25.71 ± 20.60 |
A0 | 15.71 ± 12.94 a | 6.29 ± 8.78 b |
B1 | 30.00 ± 16.90 | 22.86 ± 12.78 |
B2 | 45.71 ± 11.78 | 22.86 ± 16.66 |
B0 | 32.86 ± 12.78 | 22.86 ± 7.00 |
C1 | 24.29 ± 10.50 | 24.29 ± 15.91 |
C2 | 30.00 ± 7.56 | 37.14 ± 16.66 |
C0 | 11.43 ± 9.90 a | 0.00 ± 0.00 b |
D | 37.14 ± 13.85 | 34.29 ± 9.04 |
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Guo, M.; Liu, D.; Xu, H.; Zhang, H.; Jin, Y.; Tan, H.; Wu, Y.; Zhang, X. The Protective Efficacy of an Inactivated Vaccine against Avibacterium paragallinarum Field Isolates. Vet. Sci. 2022, 9, 458. https://doi.org/10.3390/vetsci9090458
Guo M, Liu D, Xu H, Zhang H, Jin Y, Tan H, Wu Y, Zhang X. The Protective Efficacy of an Inactivated Vaccine against Avibacterium paragallinarum Field Isolates. Veterinary Sciences. 2022; 9(9):458. https://doi.org/10.3390/vetsci9090458
Chicago/Turabian StyleGuo, Mengjiao, Donghui Liu, Hengli Xu, Hao Zhang, Yikun Jin, Huihui Tan, Yantao Wu, and Xiaorong Zhang. 2022. "The Protective Efficacy of an Inactivated Vaccine against Avibacterium paragallinarum Field Isolates" Veterinary Sciences 9, no. 9: 458. https://doi.org/10.3390/vetsci9090458