Mutational Analysis of c-KIT and PDGFRA in Canine Gastrointestinal Stromal Tumors (GISTs)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samples Collection
2.2. Histology
2.3. Immunohistochemistry
2.4. Analysis of c-KIT and PDGFRA Mutations
2.5. Statistical Analysis
3. Results
3.1. Sample Collection
3.2. Histology
3.3. Immunohistochemistry
3.4. Analysis of c-KIT and PDGFRA Mutations
3.5. Statistical Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequence 5′ → 3′ | Annealing T |
---|---|---|
Ex 8 c-KIT forward | [Hex]–GGGGAGCCTTGGTGAGGTGT | 58 °C |
Ex 8 c-KIT reverse | CCCTGCTGTCCTTCCCTCGT | 58 °C |
Ex 9 c-KIT forward | ACTCGTCTCTGTCACCGTCTGGAA | 58 °C |
Ex 9 c-KIT reverse | ATGGCAGGCAGAGCCTAAACATCC | 58 °C |
Ex 11 c-KIT forward | [6FAM] ATGATCTGTCTCTCTTTTCTCCCC | 60 °C |
Ex 11 c-KIT reverse | GTACACAAAAAGGTTACATGGAAAGC | 60 °C |
F_PDGFRA_ex 18 | GTTCCTTCCCTTTCCATGCA | 56 °C |
R_PDGFRA_ex 18 | GTGAGGAAAGGTGGGCTTGTC | 56 °C |
F_PDGFRA_ex 12 | TGCGTCTGGGCTTTGATAATT | 56 °C |
R_PDGFRA_ex 12 | GATCACCCCAGTAGGCGCTTA | 56 °C |
Case | Breed | Sex | Age, y | Location | Tumor Size (cm) | Metastasis | Histotype (WHO) [3] | Grade * | CD117 ** | PDGFR ** | SMA ** | DES ** |
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | English Setter | M | 9 | Duodenum | u | a | Fascicular | II | +++ | − | ++ | − |
2 | Collie | F | 9 | Duodenum | u | a | Storiform | I | +++ | − | − | − |
3 | German Shepherd | F | 12 | Ileociecocolic junction | u | a | Storiform | I | +++ | + | ++ | − |
4 | Mixed | u | u | Cecum | 4 | a | Epithelioid | I | +++ | ++ | ++ | − |
5 | Great Dane | F | 10 | Small intestine | u | a | Epithelioid | I | +++ | ++ | ++ | − |
6 | Mixed | M | 11 | Small intestine | 3 | a | Storiform | I | +++ | + | +++ | − |
7 | Pekingese | NM | 14 | Ileociecocolic junction | 1 | Diaphragm, liver, mesentery | Fascicular | I | +++ | − | ++ | − |
8 | Poodle | M | 13 | Stomach | 10 | Spleen | Storiform | I | +++ | + | − | − |
9 | English Setter | u | 7 | Stomach | u | a | Epithelioid | II | +++ | + | ++ | − |
10 | Maltese dog | M | 12 | Ileum | 8 | Mesentery | Fascicular | II | +++ | ++ | − | − |
11 | Schnauzer toy | FS | 14 | Jejunum | 7 × 8 | a | Epithelioid | III | +++ | + | − | − |
12 | Mixed | NM | 12 | Colon | u | a | Mixoid | I | +++ | + | − | − |
13 | Belgian Shepherd | FS | 14 | Ileum | 10 | Mesentery, lymph node | Fascicular | I | +++ | ++ | ++ | − |
14 | German Shepherd | M | 11 | Duodenum | 3 × 4 | a | Epithelioid | II | +++ | + | − | − |
15 | Corso | FS | 5 | Jejunum | 5.7 × 2 | a | Fascicular | II | +++ | ++ | ++ | − |
16 | Mixed | M | 10 | Cecum | 5 × 2 | a | Fascicular | II | +++ | + | ++ | − |
17 | Mixed | NM | 12 | Cecum | 3 × 1 × 2 | a | Fascicular | I | +++ | ++ | − | − |
Case | c-KIT | PDGFRA | ||
---|---|---|---|---|
1 | Wild-type | Wild-type | ||
2 | Wild-type | Wild-type | ||
3 | Exon 11, deletion | 6 bp | Wild-type | |
4 | Exon 11, deletion | 15 bp | Exon 18, SNP | c.2597G > A; p.866 Arg > Lys |
5 | Wild-type | Wild-type | ||
6 | Wild-type | Wild-type | ||
7 | Exon 11, deletion | 3 bp | Wild-type | |
8 | Exon 11, deletion | 6 bp | Wild-type | |
9 | Wild-type | Wild-type | ||
10 | Exon 11, deletion | 6 bp | Wild-type | |
11 | Wild-type | Wild-type | ||
12 | Exon 11, deletion | 48/49 bp | Wild-type | |
13 | Exon 11, deletion | 21 bp | Wild-type | |
14 | Wild-type | Wild-type | ||
15 | Wild-type | Wild-type | ||
16 | Exon 11, deletion | 21 bp | Wild-type | |
17 | Wild-type | Wild-type |
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Morini, M.; Gentilini, F.; Turba, M.E.; Gobbo, F.; Mandrioli, L.; Bettini, G. Mutational Analysis of c-KIT and PDGFRA in Canine Gastrointestinal Stromal Tumors (GISTs). Vet. Sci. 2022, 9, 376. https://doi.org/10.3390/vetsci9070376
Morini M, Gentilini F, Turba ME, Gobbo F, Mandrioli L, Bettini G. Mutational Analysis of c-KIT and PDGFRA in Canine Gastrointestinal Stromal Tumors (GISTs). Veterinary Sciences. 2022; 9(7):376. https://doi.org/10.3390/vetsci9070376
Chicago/Turabian StyleMorini, Maria, Fabio Gentilini, Maria Elena Turba, Francesca Gobbo, Luciana Mandrioli, and Giuliano Bettini. 2022. "Mutational Analysis of c-KIT and PDGFRA in Canine Gastrointestinal Stromal Tumors (GISTs)" Veterinary Sciences 9, no. 7: 376. https://doi.org/10.3390/vetsci9070376