The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Snail Secretion Filtrate (SSF) Collection and Sterilization
2.3. Full-Thickness Excisional Wound Model
2.4. Histopathological and Immunohistochemical Analyses
2.5. ELISA
2.6. Toluidine Blue Staining
2.7. Western Blot
2.8. Statistical Analysis
3. Results
3.1. Snail Secretion Filtrate Accelerated the Rate of Wound Healing
3.2. Effect of SSF on Skin Wound Healing and Collagen Pattern
3.3. Effect of SSF on α-sma and VEGF Expression on Wounds
3.4. Effect of SSF on Immune Cell Infiltration and Inflammatory Response
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gugliandolo, E.; Macrì, F.; Fusco, R.; Siracusa, R.; D’Amico, R.; Cordaro, M.; Peritore, A.F.; Impellizzeri, D.; Genovese, T.; Cuzzocrea, S.; et al. The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice. Vet. Sci. 2021, 8, 167. https://doi.org/10.3390/vetsci8080167
Gugliandolo E, Macrì F, Fusco R, Siracusa R, D’Amico R, Cordaro M, Peritore AF, Impellizzeri D, Genovese T, Cuzzocrea S, et al. The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice. Veterinary Sciences. 2021; 8(8):167. https://doi.org/10.3390/vetsci8080167
Chicago/Turabian StyleGugliandolo, Enrico, Francesco Macrì, Roberta Fusco, Rosalba Siracusa, Ramona D’Amico, Marika Cordaro, Alessio Filippo Peritore, Daniela Impellizzeri, Tiziana Genovese, Salvatore Cuzzocrea, and et al. 2021. "The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice" Veterinary Sciences 8, no. 8: 167. https://doi.org/10.3390/vetsci8080167