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Veterinary Sciences
Volume 8
Issue 3
10.3390/vetsci8030048
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Communication

Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes

by
Erin C. Coscia
1,
Nader S. Abutaleb
2,
Bradley Hostetter
3,
Mohamed N. Seleem
2,4,
Gert J. Breur
2,5,
Robyn R. McCain
5,
Christa J. Crain
5,
Ondrej Slaby
6,
Manu N. Capoor
7,
Andrew McDowell
8,
Fahad S. Ahmed
6,
Viju Vijayanpillai
2,
Sanjeev K. Narayanan
2 and
Michael F. Coscia
9,*
1
College of Osteopathic Medicine, Marian University, Indianapolis, IN 46222, USA
2
Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA
3
Center for Diagnostic Imaging, Carmel, IN 46032, USA
4
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
5
Center for Comparative Translational Research, Purdue University, 625 Harrison Street, West Lafayette, IN 47907, USA
6
Central European Institute of Technology (CEITEC), Masaryk University, 625 00 Brno, Czech Republic
7
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
8
Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine BT52 1SA, UK
9
OrthoIndy, 8450 Northwest Blvd, Indianapolis, IN 46278, USA
*
Author to whom correspondence should be addressed.
Vet. Sci. 2021, 8(3), 48; https://doi.org/10.3390/vetsci8030048
Submission received: 1 March 2021 / Revised: 9 March 2021 / Accepted: 13 March 2021 / Published: 16 March 2021
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Abstract

The anaerobic bacterium Cutibacterium acnes has been increasingly linked to the development of degenerative disc disease (DDD), although causality is yet to be conclusively proven. To better study how this organism could contribute to the aetiology of DDD, improved animal models that are more reflective of human disc anatomy, biology and mechanical properties are required. Against this background, our proof-of concept study aimed to be the first demonstration that C. acnes could be safely administered percutaneously into sheep intervertebral discs (IVDs) for in vivo study. Following our protocol, two sheep were successfully injected with a strain of C. acnes (8.3 × 106 CFU/disc) previously recovered from a human degenerative disc. No adverse reactions were noted, and at one-month post inoculation all triplicate infected discs in our first animal grew C. acnes, albeit at a reduced load (5.12 × 104 to 6.67 × 104 CFU/disc). At six months, no growth was detected in discs from our second animal indicating bacterial clearance. This pilot study has demonstrated the feasibility of safe percutaneous injection of C. acnes into sheep IVDs under fluoroscopic guidance. The design of follow-up sheep studies to investigate the potential of C. acnes to drive pathological changes within infected discs should now be pursued.
Keywords: Cutibacterium acnes; sheep model; spinal intervertebral discs; percutaneous injections; bacterial discitis Cutibacterium acnes; sheep model; spinal intervertebral discs; percutaneous injections; bacterial discitis

Share and Cite

MDPI and ACS Style

Coscia, E.C.; Abutaleb, N.S.; Hostetter, B.; Seleem, M.N.; Breur, G.J.; McCain, R.R.; Crain, C.J.; Slaby, O.; Capoor, M.N.; McDowell, A.; et al. Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes. Vet. Sci. 2021, 8, 48. https://doi.org/10.3390/vetsci8030048

AMA Style

Coscia EC, Abutaleb NS, Hostetter B, Seleem MN, Breur GJ, McCain RR, Crain CJ, Slaby O, Capoor MN, McDowell A, et al. Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes. Veterinary Sciences. 2021; 8(3):48. https://doi.org/10.3390/vetsci8030048

Chicago/Turabian Style

Coscia, Erin C., Nader S. Abutaleb, Bradley Hostetter, Mohamed N. Seleem, Gert J. Breur, Robyn R. McCain, Christa J. Crain, Ondrej Slaby, Manu N. Capoor, Andrew McDowell, and et al. 2021. "Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes" Veterinary Sciences 8, no. 3: 48. https://doi.org/10.3390/vetsci8030048

APA Style

Coscia, E. C., Abutaleb, N. S., Hostetter, B., Seleem, M. N., Breur, G. J., McCain, R. R., Crain, C. J., Slaby, O., Capoor, M. N., McDowell, A., Ahmed, F. S., Vijayanpillai, V., Narayanan, S. K., & Coscia, M. F. (2021). Sheep as a Potential Model of Intradiscal Infection by the Bacterium Cutibacterium acnes. Veterinary Sciences, 8(3), 48. https://doi.org/10.3390/vetsci8030048

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