Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Intestinal Markers
2.3. Histopathology
2.4. Fecal Proteomics
2.5. Statistical Analysis
3. Results
3.1. CIBDAI Score
3.2. Intestinal Markers
3.3. Histopathology
3.4. Fecal Proteomics
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Case (ILG) Mean Value ± DS | Control (CG) Mean Value ± DS | * p | |
---|---|---|---|
CIBDAI | 12.31 ± 2.75 | 1 ± 0.81 | 0.007 |
Serum | |||
Albumin | 17.32 ± 4.23 | 32.14 ± 3.84 | <0.0001 |
Cholesterol | 110.75 ± 39.89 | 212.28 ± 18.81 | <0.0001 |
CRP | 26.83 ± 16.98 | 1.21 ± 0.6 | 0.0009 |
LPS | 0.52 ± 0.30 | 0.1 ± 0.05 | 0.0001 |
cCK18 | 365.81 ± 104.92 | 148.71 ± 74.68 | 0.0001 |
Citrulline | 4.56 ± 1.59 | 2.9 ± 3.10 | 0.14 |
Zonulin | 77.27 ± 32.38 | 38.91 ± 19.10 | 0.0181 |
Feces | |||
Zonulin | 523.20 ± 156.42 | 141.56 ± 72.67 | <0.0001 |
Biopsies | |||
Histological score | 13.5 ± 2.25 | 1.85 ± 1.21 | <0.0001 |
Villous height | 623.31 ± 17.14 | 809.57 ± 26.78 | <0.0001 |
Villous width | 277.25 ± 11.03 | 151.42 ± 11.68 | <0.0001 |
Villous h/w ratio | 2.25 ± 0.11 | 5.37 ± 0.45 | <0.0001 |
Lymphatic vessels (chyliferous ducts) height | 551.18 ± 24.03 | 658.71 ± 33.50 | <0.0001 |
Lymphatic vessels (chyliferous ducts) width | 107.50 ± 42.68 | 23.85 ±2.47 | <0.0001 |
Lymphatic vessels (chyliferous ducts) h/w ratio | 5.95 ± 2.32 | 27.79 ± 3.56 | <0.0001 |
SPOT ID a | Protein Name b | Score c | Mr (kDa)/pI d | Mr (kDa)/pI e | Sequence | NQ e (×103) Healthy * | NQ (×103) IL |
---|---|---|---|---|---|---|---|
Y | Serum albumin isoform X1 (Canis lupus familiaris) | 56 | 68.6/5.51 | 72 ± 4.6/5.3 ± 0.06 | LVAAAQAALV | 115 ± 47 | 71 ± 42 |
V1 | Alkaline phosphatase (Canis lupus familiaris) | 125 | 68.6/6.47 | 59 ± 1.3/6.6 ± 0.23 | ANYQTIGVSAAAR | 174 ± 108 | 47 ± 26 |
V2 | Alkaline phosphatase (Canis lupus familiaris) | 117 | 48.3/6.15 | 58 ± 1.6/6.6 ± 0.15 | ANYQTIGVSAAAR | 148 ± 51 | 35 ± 18 |
H | Chymotrypsin-C-like (Canis lupus dingo) | 49 | 29.1/5.33 | 29 ± 1.0/5.6 ± 0.11 | LAEPVQLSDTIK | 290 ± 93 | 264 ± 27 |
H1 | Elastase-3B, proteinase E (Canis lupus familiaris) | 40 | 28.8/5.27 | 29 ± 0.8/5.2 ± 0.11 | VSAFNDWIEEVMSSH | 585 ± 139 | 616 ± 351 |
H3 | Immunoglobulin kappa light chain (Felis catus) | 41 | 26.7/6.10 | 29 ± 1.1/6.3 ± 0.21 | FSGSGSGTDFTLR | 374 ± 248 | 214 ± 32 |
G | Immunoglobulin λ-1 light chain (Canis lupus familiaris) | 34 | 25.2/6.88 | 29 ± 0.9/7.1 ± 0.5 | KGTHVTVLGQPK | 644 ± 327 | 570 ± 59 |
G1 | Immunoglobulin λ-1 light chain (Felis catus) | 39 | 27.8/8.17 | 29 ± 1.2/7.6 ± 0.6 | QSNNKYAASSYL | 555 ± 204 | 232 ± 142 |
G2 | Immunoglobulin λ-light chain VLJ region (Homo sapiens) | 42 | 29.0/8.14 | 29 ± 1.0/6.6 ± 0.3 | EFGGGTKLTVLGQPK | 642 ± 439 | 321 ± 92 |
G3 | Immunoglobulin λ-light chain VLJ region (Homo sapiens) | 30 | 29.0/8.14 | 27 ± 1.2/8.4 ± 0.5 | EFGGGTKLTVLGQPK | 395 ± 85 | 483 ± 64 |
G4 | Immunoglobulin λ-light chain VLJ region (Homo sapiens) | 40 | 29.0/8.14 | 27.7/8.9 | QSNNKYAASSYL | 285 ± 17 | 334 ± 271 |
M | Fc fragment of IgG binding protein (Homo sapiens) | 23 | 572/5.1 | 49.1 ± 2.2/5.3 ± 0.06 | VLVENEHR | n.d. | 148 ± 25 |
N | Transthyretin (Canis lupus familiaris) | 31 | 15.8/6.4 | 14.2 ± 1.2/7.8 ± 0.1 | GSPAVNVAVK | n.d. | 62 ± 58 |
P | Deleted in malignant brain tumors 1 protein isoform X1 (Canis lupus dingo) | 55 | 26/5.2 | 19.8 ± 0.15/4.2 ± 0.3 | FGQGSGPIVLDDVR | 196.5 ± 53.2 | 132 ± 39 |
S | Proproteinase E (Bos taurus) | 43 | 27.3/5.1 | 14 ± 3.4/5.8 ± 0 | LYTGGPLPDK | n.d. | 103 ± 78 |
§ * Serum albumin isoform X1 (Canis lupus familiaris) | 68.6/5.51 | 63 ± 3.0/5.8 ± 0.15 | ADFAEISK | 79 ± 13 | n.d. | ||
§ * Nuclear pore membrane glycoprotein-210 (Canis lupus familiaris) | 192.4/6.30 | 19.6 ± 1.5/5.8 ± 0.14 | TALLVTASISGSHAPR | 227 ± 69 | n.d. | ||
§ * Cytosol aminopeptidase (Canis lupus familiaris) | 56.2/8.03 | 21.0 ± 1.3/5.7 ± 0.07 | EILNISGPPLK | 125 ± 32 | n.d. |
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Rossi, G.; Gavazza, A.; Vincenzetti, S.; Mangiaterra, S.; Galosi, L.; Marchegiani, A.; Pengo, G.; Sagratini, G.; Ricciutelli, M.; Cerquetella, M. Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia. Vet. Sci. 2021, 8, 242. https://doi.org/10.3390/vetsci8100242
Rossi G, Gavazza A, Vincenzetti S, Mangiaterra S, Galosi L, Marchegiani A, Pengo G, Sagratini G, Ricciutelli M, Cerquetella M. Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia. Veterinary Sciences. 2021; 8(10):242. https://doi.org/10.3390/vetsci8100242
Chicago/Turabian StyleRossi, Giacomo, Alessandra Gavazza, Silvia Vincenzetti, Sara Mangiaterra, Livio Galosi, Andrea Marchegiani, Graziano Pengo, Gianni Sagratini, Massimo Ricciutelli, and Matteo Cerquetella. 2021. "Clinicopathological and Fecal Proteome Evaluations in 16 Dogs Presenting Chronic Diarrhea Associated with Lymphangiectasia" Veterinary Sciences 8, no. 10: 242. https://doi.org/10.3390/vetsci8100242