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Open AccessCommunication
Vet. Sci. 2018, 5(1), 8; https://doi.org/10.3390/vetsci5010008

Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand

1
School of Veterinary Science, Massey University, Palmerston North 4410, New Zealand
2
Estendart Limited, Palmerston North 4440, New Zealand
3
Massey Institute of Food Science and Technology, Massey University, Palmerston North 4410, New Zealand
*
Author to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 21 December 2017 / Accepted: 17 January 2018 / Published: 19 January 2018
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Abstract

Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from intramammary infections. Congo red agar (CRA) and microtitre test assay (MTA) in vitro phenotypic tests were used to evaluate biofilm formation. The presence of icaA, icaD, and bap genes associated with biofilm formation was confirmed using the polymerase chain reaction. Results show that most of the S. aureus isolates, though not possessing one of the biofilm-forming genes, were able to produce biofilms. MTA was more frequently positive in identifying biofilm-forming isolates than CRA. View Full-Text
Keywords: biofilms; bovine mastitis; Staphylococcus aureus; IcaA; IcaD; bap biofilms; bovine mastitis; Staphylococcus aureus; IcaA; IcaD; bap
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Notcovich, S.; DeNicolo, G.; Flint, S.H.; Williamson, N.B.; Gedye, K.; Grinberg, A.; Lopez-Villalobos, N. Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand. Vet. Sci. 2018, 5, 8.

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