Characterization of Haptoglobin Isotype in Milk of Mastitis-Affected Cows
by
Indu Upadhyaya 1,*
, Jacob Thanislass 2, Anitha Veerapandyan 2,†, Sharanabasav Badami 2,† and Prabhakar X. Antony 3
Indu Upadhyaya 1,*, Jacob Thanislass 2, Anitha Veerapandyan 2,†, Sharanabasav Badami 2,† and Prabhakar X. Antony 3
1
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
2
Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry 605009, India
3
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry 605009, India
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Patrick Butaye
Vet. Sci. 2016, 3(4), 29; https://doi.org/10.3390/vetsci3040029
Received: 28 July 2016 / Revised: 29 September 2016 / Accepted: 30 September 2016 / Published: 13 October 2016
Haptoglobin is a major acute phase protein in bovines and reportedly increases in serum and milk whey during mastitis, highlighting its potential as a diagnostic biomarker. Since haptoglobin is known to undergo tissue specific glycosylation resulting in different isoforms, this study was undertaken to characterize the isoforms of haptoglobin. Milk whey fraction and serum obtained from animals with or without clinical mastitis in Puducherry, India, were subjected to SDS-PAGE followed by western blot and immuno-detection of haptoglobin protein. All subunits (β, α1 and α2) of haptoglobin protein were detected in serum sample obtained from clinical cases. However, only the β-subunit was detected in milk whey fraction obtained from the respective animals. Similar results were observed with milk whey fractions from subclinical cases indicating difference in isoform of haptoglobin detected in milk whey from serum. This was further supported by RT-PCR (Reverse Transcription Polymerase Chain Reaction) analysis of haptoglobin gene (Hp) confirming the tissue specific origin of haptoglobin.
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Keywords:
haptoglobin; mastitis; subclinical; diagnosis; local origin; biomarker
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MDPI and ACS Style
Upadhyaya, I.; Thanislass, J.; Veerapandyan, A.; Badami, S.; Antony, P.X. Characterization of Haptoglobin Isotype in Milk of Mastitis-Affected Cows. Vet. Sci. 2016, 3, 29.
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