p16, pRb, and p53 in Feline Oral Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Immunostaining
2.3. p16 mRNA in 3 FOSCC Cell Lines
2.4. Papillomavirus L1 Major Capsid DNA Investigation
2.5. PCR Product Purification and Sequencing
2.6. Statistical Analysis
3. Results
3.1. Relative p16 mRNA Quantification in 3 FOSCC Cell Lines
3.2. Immunohistochemistry (IHC)
3.3. Papillomavirus L1 capsid DNA amplification:
3.4. Sequencing
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
FOSCC | Feline oral squamous cell carcinoma |
HPV | Human papillomavirus |
PV | Papillomavirus |
FcaPV | Feline catus papillomavirus |
IHC | Immunohistochemistry |
FFPE | Formalin-fixed, paraffin-embedded |
PCR | Polymerase chain reaction |
RT-PCR | Reverse transcriptase PCR |
HNSCC | Head and neck squamous cell carcinoma |
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Protein | Absent to Low | Moderate | High |
---|---|---|---|
p16 | <20% of cancer cells have low cytoplasmic intensity | 20%–50% of cancer cells have low to intermediate cytoplasmic and nuclear staining intensity | >50% of cancer cells have high cytoplasmic and nuclear staining |
pRb and p53 | <20% of cancer cells have low to intermediate nuclear staining intensity | 20%–50% of cancer cells have high nuclear staining intensity, or >50% of cancer cells have low to intermediate nuclear staining intensity | >50% of cancer cells have high nuclear staining intensity |
Signalment | IHC Results | ||||||
---|---|---|---|---|---|---|---|
No. | Breed | Sex | Age (Year) | Location | p16 | pRb | p53 |
1 | DSH | FS | 14 | Gingiva | Low | Moderate | Low |
2 | DSL | MC | 20 | Gingiva | Low | Low | N/A |
3 | DSH | F | 11 | Gingiva | High | High | Low |
4 | Himalayan | FS | 16 | Gingiva | Moderate | Moderate | Low |
5 | DSH | MC | 15 | Gingiva | Moderate | Moderate | Moderate |
6 | DSH | FS | N/A | Gingiva | Moderate | Moderate | Low |
7 | Himalayan | MC | 12 | Gingiva | Low | High | Low |
8 | DSH | MC | 11 | Gingiva | Moderate | Moderate | High |
9 | DSH | MC | 17 | Gingiva | Moderate | Moderate | Low |
10 | DSH | MC | 13 | Tongue | Low | High | High |
11 | Siamese | FS | 13 | Tongue | Low | Low | Moderate |
12 | DSH | MC | 16 | Tongue | Low | Moderate | Low |
13 | DLH | MC | 11 | Tongue | Moderate | Low | Low |
14 | DSH | FS | 13 | Tongue | Low | Low | Low |
15 | Exotic SH | F | 10 | Tongue | Moderate | N/A | Low |
16 | DSH | MC | 11.5 | Tongue | Moderate | Moderate | Low |
17 | DLH | F | 10 | Tongue | Moderate | Moderate | Low |
18 | DSH | MC | 6 | Tongue | Low | Low | High |
19 | DSH | FS | 15.5 | Tongue | Low | Low | High |
20 | DSH | F | 10 | Tongue | Moderate | Moderate | Low |
21 | DSH | F | 12 | Sublingual | Low | Low | Moderate |
22 | Angora | FS | 7 | Sublingual | Low | Moderate | Low |
23 | DSH | MC | 4 | Sublingual | Moderate | Low | Low |
24 | DSH | M | 14+ | Sublingual | Moderate | Low | High |
25 | Persian | FS | 13.5 | Sublingual | High | Moderate | Low |
26 | DSH | MC | 12 | Sublingual | Moderate | Moderate | Low |
27 | DSH | M | 12.5 | Sublingual | Low | Low | Low |
28 | DLS | FS | 17 | Sublingual | Low | High | High |
29 | DSH | FS | 19 | Sublingual | Low | Moderate | Low |
30 | DSH | FS | 14 | Sublingual | Moderate | Moderate | Low |
31 | DLH | MC | 17 | Sublingual | Low | Moderate | Low |
32 | DSH | FS | 19 | Sublingual | Low | N/A | Low |
33 | DSH | MC | 13 | Mandi or Maxi | High | Moderate | High |
34 | DSH | MC | 12 | Mandi or Maxi | High | Low | Low |
35 | DLH | F | 6.5 | Mandi or Maxi | Moderate | Moderate | Low |
36 | DSH | MC | 12 | Mandi or Maxi | High | Moderate | Low |
37 | DSH | F | 16 | Mandi or Maxi | Low | Low | Low |
38 | DSH | F | 20 | Mandi or Maxi | Low | Low | Low |
39 | DSH | FS | 2 | Mandi or Maxi | High | Moderate | Low |
40 | DLH | MC | 14 | Mandi or Maxi | Moderate | Low | Low |
41 | Himalayan | F | 11 | Mandi or Maxi | Moderate | Moderate | Low |
42 | DSH | MC | 13 | Mandi or Maxi | Moderate | Moderate | Low |
43 | DSH | FS | 9 | Mandi or Maxi | Moderate | High | N/A |
Sample | p16 IHC | p53 IHC | pRB IHC |
---|---|---|---|
SCCF1 | High | Low | Low |
SCCF2 | Low | Low | Low |
SCCF3 | Low | Moderate | Low |
FBISC | High | Low | Low |
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Supsavhad, W.; Dirksen, W.P.; Hildreth, B.E.; Rosol, T.J. p16, pRb, and p53 in Feline Oral Squamous Cell Carcinoma. Vet. Sci. 2016, 3, 18. https://doi.org/10.3390/vetsci3030018
Supsavhad W, Dirksen WP, Hildreth BE, Rosol TJ. p16, pRb, and p53 in Feline Oral Squamous Cell Carcinoma. Veterinary Sciences. 2016; 3(3):18. https://doi.org/10.3390/vetsci3030018
Chicago/Turabian StyleSupsavhad, Wachiraphan, Wessel P. Dirksen, Blake E. Hildreth, and Thomas J. Rosol. 2016. "p16, pRb, and p53 in Feline Oral Squamous Cell Carcinoma" Veterinary Sciences 3, no. 3: 18. https://doi.org/10.3390/vetsci3030018