Study of Oxidant/Antioxidant Profile in Dogs with Mammary Cancer Undergoing Mastectomy, During the Peri-Operative Period †
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Anesthetic Protocol
2.3. Histological Evaluation
2.4. Blood Measurements
2.5. Statistical Analysis
3. Results
3.1. Animals: Clinical and Histological Characteristics of Mammary Tumors
3.2. Blood Measurements
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Hogan, B.V.; Peter, M.B.; Shenoy, H.G.; Horgan, K.; Hughes, T.A. Surgery induced immunosuppression. Surgeon 2011, 9, 38–43. [Google Scholar] [CrossRef] [PubMed]
- Alieva, M.; van Rheenen, J.; Broekman, M.L.D. Potential impact of invasive surgical procedures on primary tumor growth and metastasis. Clin. Exp. Metastasis 2018, 35, 319–331. [Google Scholar] [CrossRef]
- Kim, R. Effects of surgery and anesthetic choice on immunosuppression and cancer recurrence. J. Transl. Med. 2018, 16, 8. [Google Scholar] [CrossRef]
- Perry, J.A.; Douglas, H. Immunomodulatory effects of surgery, pain, and opioids in cancer patients. Vet. Clin. Small Anim. 2019, 49, 981–991. [Google Scholar] [CrossRef]
- Onuma, A.E.; Zhang, H.; Gil, L.; Huang, H.; Tsung, A. Surgical stress promotes tumor progression: A focus on the impact of the immune response. J. Clin. Med. 2020, 9, 4096. [Google Scholar] [CrossRef]
- Tang, F.; Tie, Y.; Tu, C.; Wei, X. Surgical trauma-induced immunosuppression in cancer: Resent advances and the potential therapies. Clin. Transl. Med. 2020, 10, 199–223. [Google Scholar] [CrossRef]
- Karayannopoulou, M.; Anagnostou, T.; Margariti, A.; Kritsepi-Konstantinou, M.; Psalla, D.; Savva, I.; Kazakos, G. Effect of anaesthesia for mastectomy on cell-mediated immunity in dogs with mammary cancer. Vet. Anaesth. Analg. 2022, 49, 265–274. [Google Scholar] [CrossRef]
- Karayannopoulou, M.; Anagnostou, T.; Margariti, A.; Kritsepi-Konstantinou, M.; Psalla, D.; Thomas, A.-L.; Savvas, I. Effect of major versus minor mastectomy on host immunity in canine mammary cancer. Vet. Immunol. Immunopathol. 2022, 246, 110403. [Google Scholar] [CrossRef]
- Brown, N.S.; Bicknell, R. Hypoxia and oxidative stress in breast cancer. Oxidative stress: Its effects on the growth, metastatic potential and response to therapy of breast cancer. Breast Cancer Res. 2001, 3, 323–327. [Google Scholar] [CrossRef]
- O’Leary, D.P.; Wang, J.H.; Cotter, T.G.; Redmond, H.P. Less stress, more success? Oncological implications of surgery-induced oxidative stress. Gut 2013, 62, 461–470. [Google Scholar] [CrossRef]
- Moloney, J.N.; Cotter, T.G. ROS signaling in the biology of cancer. Semin. Cell Dev. Biol. 2018, 80, 50–64. [Google Scholar] [CrossRef] [PubMed]
- Irwin, M.G.; Chung, C.K.E.; Ip, K.Y.; Wiles, M.D. Influence of propofol-based total intravenous anaesthesia on peri-operative outcome measures: A narrative review. Anaesthesia 2020, 75, e90–e100. [Google Scholar] [CrossRef] [PubMed]
- Reuter, S.; Gupta, S.C.; Chaturvedi, M.M.; Aggarwal, B.B. Oxidative stress, inflammation and cancer: How are they linked? Free Radic. Biol. Med. 2010, 49, 1603–1616. [Google Scholar] [CrossRef]
- Lee, J.Y. Oxidative stress due to anesthesia and surgical trauma and comparison of the effects of propofol and thiopental in dogs. J. Vet. Med. Sci. 2012, 74, 663–665. [Google Scholar] [CrossRef]
- Lee, Y.-M.; Song, B.C.; Yeum, K.-J. Impact of volatile anesthetics on oxidative stress and inflammation. Biomed. Res. Int. 2015, 2015, 242709. [Google Scholar] [CrossRef]
- Lee, J.D.; Cai, Q.; Ou Shu, X.; Nechuta, S.J. The role of biomarkers of oxidative stress in breast cancer risk and prognosis: A systematic review of the epidemiologic literature. J. Women’s Health 2017, 26, 467–482. [Google Scholar] [CrossRef]
- Jelik, M.D.; Mandic, A.D.; Maricic, S.M.; Srdjenovic, B.U. Oxidative stress and its role in cancer. J. Can. Res. Ther. 2021, 17, 22–28. [Google Scholar] [CrossRef]
- Zahra, K.F.; Lefter, R.; Ali, A.; Abdellah, E.-C.; Trus, K.; Ciobica, A.; Timofte, D. The involvement of the oxidative stress status in cancer pathology: A double view on the role of the antioxidants. Oxid. Med. Cell Longev. 2021, 2021, 9965916. [Google Scholar] [CrossRef]
- Calaf, G.M.; Urzua, U.; Termini, L.; Aguayo, F. Oxidative stress in female cancers. Oncotarget 2018, 9, 23824–23842. [Google Scholar] [CrossRef]
- Marioli-Sapsakou, G.-K.; Kourti, M. Targeting production of reactive oxygen species as an anticancer strategy. Anticancer Res. 2021, 41, 5881–5902. [Google Scholar] [CrossRef]
- Stavinohová, R.; Lorenzová, J.; Papežiková, I.; Borkovcová, I.; Pfeifr, J.; Lojek, A.; Mrázová, M.; Crha, M. Markers of oxidative and antioxidative activity in female dogs with mammary gland tumour with and without additional vitamin E supplementation. Acta Vet. Brno 2012, 81, 275–280. [Google Scholar] [CrossRef]
- Macotpet, A.; Suksawat, F.; Sukon, P.; Pimpakdee, K.; Pattarapanwichien, E.; Tangrassameeprasert, R.; Boonsiri, P. Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Vet. Res. 2013, 9, 101. [Google Scholar] [CrossRef]
- Machado, V.S.; Crivellenti, L.Z.; Bottari, N.B.; Tonin, A.A.; Pelinson, L.P.; Borin-Crivellenti, S.; Santana, A.E.; Torbitz, V.D.; Moresco, R.N.; Duarte, T.; et al. Oxidative stress and inflammatory response biomarkers in dogs with mammary carcinoma. Pathol.-Res. Pract. 2015, 211, 677–681. [Google Scholar] [CrossRef]
- Russo, C.; Simonelli, S.M.; Luz, M.B.; Carrera, A.C.; Moreno, I.F.; Bracarense, A.P.F.R.L. Oxidative stress in female dogs with mammary neoplasms. Pesq. Vet. Bras. 2021, 41, e06742. [Google Scholar] [CrossRef]
- Bhattacharjee, J.; Jongdand, S.; Shind, R.K.; Goswami, S. Assessment of oxidative stress in breast cancer patients: A hospital based study. Int. J. Basic Clin. Pharmacol. (IJBCP) 2018, 7, 966–970. [Google Scholar] [CrossRef]
- Hewala, T.I.; Abo Elsoud, M.R. The clinical significance of serum oxidative stress biomarkers in breast cancer females. Med. Res. J. 2019, 4, 1–7. [Google Scholar]
- Hoseini, H.; Saravi, M.K.; Shahraki, S.; Sarani, A.; Sarabandi, A. Evaluation of oxidative stress biomarkers in patients with breast cancer compared with healthy subjects. J. Chem. Health Risks (JCHR) 2021, 11, 13–17. [Google Scholar]
- Pantelimon, I.; Gales, L.N.; Zgura, A.; Serbanescu, G.L.; Georgescu, D.E.; Nita, I.; Manolescu, L.S.C.; Stancu, A.M.; Gruia, M.I.; Anghel, R.M.; et al. Analysis of oxidative stress in patients with breast cancer and obesity. Ann. Med. Health Sci. Res. 2021, 11, 1578–1585. [Google Scholar]
- Danesh, H.; Ziamajidi, N.; Mesbah-Namin, S.A.; Nafisi, N.; Abbasalipourkabir, R. Association between oxidative stress parameters and hematological indices in breast cancer patients. Int. J. Breast Cancer 2022, 2022, 1459410. [Google Scholar] [CrossRef]
- Freeman, L.M. Antioxidants in cancer treatment: Helpful or harmful? Compend. Contin. Educ. Vet. 2009, 31, 154–158. [Google Scholar]
- Morry, J.; Ngamcherdtrakul, W.; Yantasee, W. Oxidative stress in cancer and fibrosis: Opportunity for therapeutic intervention with antioxidant compounds, enzymes, and nanoparticles. Redox Biol. 2017, 11, 240–253. [Google Scholar] [CrossRef] [PubMed]
- Owen, L.N. The TNM Classification of Tumors in Domestic Animals, 1st ed.; World Health Organization: Geneva, Switzerland, 1980; pp. 20–80. [Google Scholar]
- Papazoglou, L.G.; Basdani, E.; Rabidi, S.; Patsikas, M.N.; Karayannopoulou, M. Current surgical options for mammary tumor removal in dogs. J. Veter. Sci. Med. 2014, 2, 6. [Google Scholar] [CrossRef]
- Saganuwan, S.A. Derivation of a unique body surface area (bsa) formula for calculation of relatively safe doses of dog and human anticancer drugs. J. Cancer Sci. Ther. 2017, 9, 690–704. [Google Scholar] [CrossRef]
- Misdorp, W.; Else, R.W.; Hellmén, E.; Lipscomb, T.P. Histological Classification of Mammary Tumours of the Dog and the Cat, Vol. VII; Armed Forces Institute of Pathology and American Registry of Pathology and the World Health Organization Collaborating Center for Worldwide Reference on Comparative Oncology: Washington, DC, USA, 1999; pp. 11–29. [Google Scholar]
- Alberti, A.; Bolognini, L.; Macciantelli, D.; Caratelli, M. The radical cation of N,N-diethyl-para-phenylendiamine: A possible indicator of oxidative stress in biological samples. Res. Chem. Intermed. 2000, 26, 253–267. [Google Scholar] [CrossRef]
- Pasquini, A.; Luchetti, E.; Marchetti, V.; Cardini, G.; Iorio, E.L. Analytical performances of d-ROMs test and BAP test in canine plasma. Definition of the normal range in healthy Labrador dogs. Vet. Res. Commun. 2008, 32, 137–143. [Google Scholar] [CrossRef]
- Jansen, E.; Ruskovska, T. Serum biomarkers of (anti)oxidant status for epidemiological studies. Int. J. Mol. Sci. 2015, 16, 27378–27390. [Google Scholar] [CrossRef]
- Oikonomodis, I.L. Study of the Changes in Haematological, Biochemical and Hormonal Parameters, as Well as the Redox Status of the Blood of Rams During Reproductive Activity. Ph.D. Thesis, Aristotle University of Thessaloniki (A.U.Th.), Thessaloniki, Greece, 2017. [Google Scholar]
- Finotello, R.; Pasquini, A.; Meucci, V.; Lippi, I.; Rota, A.; Guidi, G.; Marchetti, V. Redox status evaluation in dogs affected by mast cell tumour. Vet. Comp. Oncol. 2012, 12, 120–129. [Google Scholar] [CrossRef]
- Jansen, E.H.J.M.; Beekhof, P.K.; Viezeliene, D.; Muzakova, V.; Skalicki, J. Long-term stability of oxidative stress biomarkers in human serum. Free Radic. Res. 2017, 51, 970–977. [Google Scholar] [CrossRef]
- Zengin, I.; Koçer, B.; Demiray, T.; Yilmaz, K. Prognostic value and significance of oxidative stress in breast cancer. Online Turk. J. Health Sci. (OTJHS) 2022, 7, 453–459. [Google Scholar] [CrossRef]
- Hiller, J.G.; Perry, N.J.; Poulogiannis, G.; Riedel, G.B.; Sloan, E.K. Perioperative events influence cancer recurrence risk after surgery. Nat. Rev. Clin. Oncol. 2018, 15, 205–218. [Google Scholar] [CrossRef]
- Enginler, S.O.; Toydemir, T.S.F.; Ates, A.; Ozturk, B.; Erdogan, O.; Ozdemir, S.; Kirsan, I.; Or, M.E.; Arun, S.S.; Barutsu, U.B. Examination of oxidative/antioxidative status and trace element levels in dogs with mammary tumors. Bulg. J. Agric. Sci. 2015, 21, 1086–1091. [Google Scholar]
Time | d-ROMs (CARR U); Mean ± Standard Deviation |
---|---|
1st day/pre | 89.50 ± 18.9 a,b |
1st day/post | 95.33 ± 23.6 c |
2nd day | 105.08 ± 25.2 a |
3rd day | 119.25 ± 20.3 b,c |
10th day | 104.42 ± 25.4 |
Time | BAP (μmol/L); Mean ± Standard Deviation |
---|---|
1st day/pre | 1218.82 ± 605.1 |
1st day/post | 1406.63 ± 725.9 |
2nd day | 1070.46 ± 577.2 |
3rd day | 1214.73 ± 631.7 |
10th day | 1299.12 ± 654.5 |
Time | OSI Value (AU); Mean ± Standard Deviation |
---|---|
1st day/pre | 107.39 ± 94.8 |
1st day/post | 175.14 ± 355.6 |
2nd day | 198.18 ± 332.2 |
3rd day | 117.27 ± 49.7 |
10th day | 99.99 ± 57.3 |
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Thomas, A.-L.; Karayannopoulou, M.; Anagnostou, T.; Psalla, D.; Ioannou, K.; Ginoudis, A.; Savvas, I.; Pardali, D. Study of Oxidant/Antioxidant Profile in Dogs with Mammary Cancer Undergoing Mastectomy, During the Peri-Operative Period. Vet. Sci. 2025, 12, 562. https://doi.org/10.3390/vetsci12060562
Thomas A-L, Karayannopoulou M, Anagnostou T, Psalla D, Ioannou K, Ginoudis A, Savvas I, Pardali D. Study of Oxidant/Antioxidant Profile in Dogs with Mammary Cancer Undergoing Mastectomy, During the Peri-Operative Period. Veterinary Sciences. 2025; 12(6):562. https://doi.org/10.3390/vetsci12060562
Chicago/Turabian StyleThomas, Angelos-Lauris, Maria Karayannopoulou, Tilemachos Anagnostou, Dimitra Psalla, Konstantinos Ioannou, Argyrios Ginoudis, Ioannis Savvas, and Dimitra Pardali. 2025. "Study of Oxidant/Antioxidant Profile in Dogs with Mammary Cancer Undergoing Mastectomy, During the Peri-Operative Period" Veterinary Sciences 12, no. 6: 562. https://doi.org/10.3390/vetsci12060562
APA StyleThomas, A.-L., Karayannopoulou, M., Anagnostou, T., Psalla, D., Ioannou, K., Ginoudis, A., Savvas, I., & Pardali, D. (2025). Study of Oxidant/Antioxidant Profile in Dogs with Mammary Cancer Undergoing Mastectomy, During the Peri-Operative Period. Veterinary Sciences, 12(6), 562. https://doi.org/10.3390/vetsci12060562