Prevalence and Genotyping of Mycobacterium avium subsp. paratuberculosis in Sheep from Inner Mongolia, China
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Areas and Sample Collection
2.2. DNA Extraction
2.3. qPCR
2.4. Statistical Analysis
3. Results
3.1. Infection Status of MAP
3.2. Genotype Identification MAP
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Factor | Sample Size | No. Positive (%) (95% CI) | p Value | OR (95% CI) | C Type | S Type | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. Positive (%) (95% CI) | p Value | OR (95% CI) | No. Positive (%) (95% CI) | p Value | OR (95% CI) | |||||||||
Breed | Sheep | 1346 | 51 (3.79%) (2.77–4.81) | 0.017 | 4.667 (1.128–l19.299) | 44 (3.27%) (2.32–4.22) | 0.036 | 4.005 (0.964–16.631) | 7 (0.52%) (0.14–0.90) | 0.603 | – | |||
Goat | 239 | 2 (0.84%) (−0.33–2.00) | 2 (0.84%) (−0.33–2.00) | 0 | ||||||||||
Feeding method | Intensive | 903 | 31 (3.56%) (2.24–4.62) | 0.888 | 0.938 (0.538–1.634) | 28 (3.10%) (1.97–4.24) | 0.879 | 1.084 (0.595–1.978) | 3 (0.33%) (0.04–0.71) | 0.472 | 0.565 (0.126–2.533) | |||
Free range | 682 | 22 (3.23%) (1.90–4.56) | 18 (2.64%) (1.43–3.85) | 4 (0.59%) (0.01–1.16) | ||||||||||
Regions | Eastern region | Hinggan League | 194 | 15 (7.73%) | 654 | 31 (4.74%) a (3.11–6.37) | 0.432 | 1.351 (0.709–2.571) | 29 (4.43%) a (2.85–6.02) | 0.087 | 1.985 (0.930–4.238) | 2 (0.31%) ab (−0.12–0.73) | 0.111 | 0.239 (0.046–1.236) |
Hulun Buir | 160 | 4 (2.50%) | ||||||||||||
Chifeng | 100 | 0 (0%) | ||||||||||||
Tongliao | 200 | 12 (6.00%) | ||||||||||||
Central region | Hohhot | 184 | 3 (1.63%) | 394 | 14 (3.55%) ab (1.72–5.39) | 0.002 | 3.290 (1.500–7.220) | 9 (1.08%) ab (0.80–3.77) | 0.004 | 3.068 (1.391–6.769) | 5 (1.30%) b (0.16–2.38) | 0.504 | – | |
Xilingol League | 100 | 4 (4.00%) | ||||||||||||
Ulanqab | 110 | 7 (6.36%) | ||||||||||||
Western region | Alxa League | 98 | 0 (0%) | 537 | 8 (1.49%) b (0.46–2.52) | 0.049 | 2.436 (1.012–5.865) | 8 (1.49%) b (0.46–2.52) | 0.459 | 1.546 (0.591–4.043) | 0 a | 0.012 | – | |
Ordos | 100 | 2 (2.00%) | ||||||||||||
Baotou | 100 | 0 (0%) | ||||||||||||
Wuhai | 84 | 1 (1.19%) | ||||||||||||
Bayannur | 155 | 5 (3.22%) | ||||||||||||
Total | 1585 | 3.34% (53/1585) | 2.90% (46/1585) | 0.44% (7/1585) |
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Zhang, R.; Lv, Y.-R.; Yang, B.; Wang, H.; Jia, J.-T.; Wu, Z.-H.; Nie, M.; Sun, L.-Y.; Xue, S.-Y.; Ding, Y.-L.; et al. Prevalence and Genotyping of Mycobacterium avium subsp. paratuberculosis in Sheep from Inner Mongolia, China. Vet. Sci. 2025, 12, 326. https://doi.org/10.3390/vetsci12040326
Zhang R, Lv Y-R, Yang B, Wang H, Jia J-T, Wu Z-H, Nie M, Sun L-Y, Xue S-Y, Ding Y-L, et al. Prevalence and Genotyping of Mycobacterium avium subsp. paratuberculosis in Sheep from Inner Mongolia, China. Veterinary Sciences. 2025; 12(4):326. https://doi.org/10.3390/vetsci12040326
Chicago/Turabian StyleZhang, Rong, Yue-Rong Lv, Bo Yang, Hao Wang, Jun-Tao Jia, Zhi-Hong Wu, Ming Nie, Lian-Yang Sun, Shi-Yuan Xue, Yu-Lin Ding, and et al. 2025. "Prevalence and Genotyping of Mycobacterium avium subsp. paratuberculosis in Sheep from Inner Mongolia, China" Veterinary Sciences 12, no. 4: 326. https://doi.org/10.3390/vetsci12040326
APA StyleZhang, R., Lv, Y.-R., Yang, B., Wang, H., Jia, J.-T., Wu, Z.-H., Nie, M., Sun, L.-Y., Xue, S.-Y., Ding, Y.-L., Chen, R.-B., Tunala, S., Zhao, L., & Liu, Y.-H. (2025). Prevalence and Genotyping of Mycobacterium avium subsp. paratuberculosis in Sheep from Inner Mongolia, China. Veterinary Sciences, 12(4), 326. https://doi.org/10.3390/vetsci12040326