Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Animals and Blood Sample Collection
2.3. Transport Vehicle and Environmental Conditions
2.4. Cortisol and 5-HT Analyses
2.5. Peripheral Blood Mononuclear Cells’ (PBMC) Isolation, RNA Extraction and Reverse Transcription
2.6. Real-Time Quantitative PCR (qPCR)
2.7. Analysis of Hematochemical Parameters
2.8. Statistical Analysis
3. Results
3.1. Circulating Cortisol and 5-HT Concentrations
3.2. 5-HT1B and 5-HT2A Serotoninergic Receptors’ Expression and IL-12 Expression
3.3. Hematochemical Parameters
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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T0 | T1 | |
---|---|---|
TG (mg/dL) | 42.6 ± 9.6 | 48.9 ± 7.7 |
TCHOL (mg/dL) | 98.0 ± 7.6 | 76.8 ± 9.5 |
AST (U/L) | 108.2 ± 23.0 | 70.0 ± 17.3 |
CPK (U/L) | 93.3 ± 34.6 | 120.1 ± 25.1 |
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Bruschetta, G.; Zanghì, G.; Giunta, R.P.; Ferlazzo, A.M.; Satué, K.; D’Ascola, A.; Fazio, E. Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses. Vet. Sci. 2024, 11, 113. https://doi.org/10.3390/vetsci11030113
Bruschetta G, Zanghì G, Giunta RP, Ferlazzo AM, Satué K, D’Ascola A, Fazio E. Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses. Veterinary Sciences. 2024; 11(3):113. https://doi.org/10.3390/vetsci11030113
Chicago/Turabian StyleBruschetta, Giuseppe, Gabriella Zanghì, Renato Paolo Giunta, Alida Maria Ferlazzo, Katiuska Satué, Angela D’Ascola, and Esterina Fazio. 2024. "Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses" Veterinary Sciences 11, no. 3: 113. https://doi.org/10.3390/vetsci11030113
APA StyleBruschetta, G., Zanghì, G., Giunta, R. P., Ferlazzo, A. M., Satué, K., D’Ascola, A., & Fazio, E. (2024). Short Road Transport and Slaughter Stress Affects the Expression Profile of Serotonin Receptors, Adrenocortical, and Hematochemical Responses in Horses. Veterinary Sciences, 11(3), 113. https://doi.org/10.3390/vetsci11030113