Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Local and Animals
2.2. Semen Extender, Antioxidant Supplementation and Experimental Design
2.3. Semen Collection and Processing
2.4. Seminal Assessment
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameters | Fixed Variables | Interactions | |
---|---|---|---|
Semen Processing (SP) | Group (G) | SP × G | |
IM | *** | NS | NS |
Alive | *** | NS | NS |
Death | *** | NS | NS |
Abnormal | ** | NS | NS |
Head defects | *** | NS | NS |
IP defects | NS | NS | NS |
Tail defects | *** | NS | NS |
TS | *** | * | NS |
TM | *** | NS | NS |
TPM | *** | ** | *** |
Slow | *** | * | NS |
Medium | *** | * | * |
Rapid | *** | NS | NS |
VCL | *** | * | * |
VSL | *** | *** | *** |
VAP | *** | *** | ** |
ALH | *** | NS | NS |
LIN | ** | NS | ** |
STR | *** | *** | NS |
WOB | *** | ** | NS |
BCF | *** | * | * |
Parameters | G1 (Control) | G2 (Melatonin) | G3 (GSH) | G4 (GSH + Melatonin) | ±(Sqrt)SEM | ||||
---|---|---|---|---|---|---|---|---|---|
Fresh | Thawed | Fresh | Thawed | Fresh | Thawed | Fresh | Thawed | ||
Alive (%) | 78.7 a | 40.1 b | 79.1 a | 38.1 b | 80.9 a | 40.9 b | 82.9 a | 41.1 b | 0.17 |
Death (%) | 20.9 a | 58.8 b | 20.0 a | 61.7 b | 18.6 a | 58.8 b | 16.6 a | 58.4 b | 0.22 |
IM (%) | 60.0 a | 39.7 b | 58.7 a | 37.2 b | 58.7 a | 40.3 b | 59.3 a | 38.0 b | 0.08 |
Abnormal (%) | 15.3 a | 11.6 a | 15.5 a | 11.4 a | 20.3 a | 10.8 a | 15.0 a | 12.9 a | 0.35 |
Head D (%) | 0.8 a,b | 2.6 a,b | 1.2 a,b | 1.7 a,b | 1.1 a,b | 1.7 a,b | 0.7 b | 3.0 a | 0.37 |
IP D (%) | 3.1 a | 3.8 a | 1.9 a | 2.7 a | 2.2 a | 2.2 a | 1.5 a | 2.1 a | 0.24 |
Tail D (%) | 9.6 a,b,c | 3.8 c | 10.9 a,b | 5.3 b,c | 15.3 a | 5.5 b,c | 11.0 a,b | 6.3 b,c | 0.40 |
Parameters | G1 (Control) | G2 (Melatonin) | G3 (GSH) | G4 (GSH + Melatonin) | ±(Sqrt)SEM | ||||
---|---|---|---|---|---|---|---|---|---|
Fresh | Thawed | Fresh | Thawed | Fresh | Thawed | Fresh | Thawed | ||
TS | 4.1 a | 37.6 b | 2.7 a | 35.3 b | 4.4 a | 38.9 b | 2.9 a | 36.8 b | 0.17 |
TM | 93.4 a | 59.5 b | 95.4 a | 62.3 b | 94.0 a | 58.2 b | 95.4 a | 61.1 b | 0.11 |
TPM | 49.2 a | 20.5 b | 40.5 c | 26.7 d | 39.7 c | 20.8 b | 37.2 c | 23.6 b,d | 0.21 |
Slow | 0.5 a | 3.4 b | 0.4 a | 3.2 b | 2.3 b | 3.7 b | 0.4 a | 4.5 b | 0.23 |
Medium | 3.5 b,c | 6.1 a | 3.5 b,c | 5.5 a,b | 5.8 a,b | 5.9 a,b | 2.6 c | 5.8 a,b | 0.20 |
Rapid | 87.1 a | 46.9 b | 89.0 a | 50.3 b | 83.0 a | 44.0 b | 89.5 a,b | 44.8 b | 0.21 |
VCL | 222.0 a | 137.2 c | 211.3 a,b | 147.5 c | 197.8 b | 136.3 c | 216.6 a,b | 135.8 c | 0.29 |
VSL | 85.8 a | 49.5 c,d | 75.3 b | 56.6 c | 69.0 b | 49.0 d | 75.0 b | 52.5 c,d | 0.19 |
VAP | 110.6 a | 70.9 c | 104.5 a,b | 76.3 c | 94.6 b | 68.9 c | 107.3 a | 71.0 c | 0.20 |
ALH | 3.6 a | 2.9 b | 3.6 a | 2.9 b | 3.5 a | 2.9 b | 3.6 a | 2.8 b | 0.03 |
LIN | 38.2 a | 36.0 a,b | 35.4 a,b | 38.3 a | 34.5 b | 35.9 a,b | 34.7 b | 38.5 a | 0.09 |
STR | 77.2 a | 69.7 b,c | 71.5 b,c | 74.1 a,b | 72.5 b,c | 70.8 b,c | 69.6 c | 73.3 a,b,c | 0.09 |
WOB | 49.4 b,c,d | 51.3 a,b,c | 49.2 c,d | 51.5 a,b | 47.5 d | 50.4 a,b,c | 49.4 b,c,d | 52.2 a | 0.05 |
BCF | 22.8 a | 18.5 c,d | 20.8 a,b | 19.7 b,c | 20.8 a,b | 17.6 d | 21.4 a,b | 19.2 b,c,d | 0.09 |
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Carriço, C.; Barbas, J.P.; Pimenta, J.; Simões, J. Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing. Vet. Sci. 2023, 10, 446. https://doi.org/10.3390/vetsci10070446
Carriço C, Barbas JP, Pimenta J, Simões J. Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing. Veterinary Sciences. 2023; 10(7):446. https://doi.org/10.3390/vetsci10070446
Chicago/Turabian StyleCarriço, Cristiana, João Pedro Barbas, Jorge Pimenta, and João Simões. 2023. "Effect of In Vitro Addition of Melatonin and Glutathione on Seminal Parameters of Rams in Diluted Semen and after Thawing" Veterinary Sciences 10, no. 7: 446. https://doi.org/10.3390/vetsci10070446