Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Case History and Samples Collection
2.2. RNA Extraction
2.3. Detection of Canine Distemper Virus RNA
2.4. CDV Sequence and Phylogenetic Analyses
2.5. Bacteriological, Parasitological, and Mycological Analyses
3. Results
3.1. Clinical Examination
3.2. CDV Detection and RFLP Analysis
3.3. Sequence and Phylogenetic Analysis
3.4. Bacteriological, Parasitological, and Mycological Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Method | Primer Sequence (5′-3′) | Target | Size | Reference |
---|---|---|---|---|
RT-PCR | DMV 1: ATG TTT ATG ATC ACA GCG GT’ DMV 2: ATT GGG TTG CAC CAC TTG TC | P gene | 429 bp | [30] |
RT-PCR/RFLP | B-for: AGG CCG TAC ATC ACC AAG TC B-rev: TGG TAA GCC ATC CGG AGT TC | H gene | 1110 bp | [31] |
Sequence analysis | C-for: AAC TTA GGG CTC AGG TAG TC C-rev: AGA TGG ACC TCA GGG TAT AG | H gene | 2023 bp | [31] |
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Guercio, A.; Mira, F.; Di Bella, S.; Gucciardi, F.; Lastra, A.; Purpari, G.; Castronovo, C.; Pennisi, M.; Di Marco Lo Presti, V.; Rizzo, M.; et al. Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo). Vet. Sci. 2023, 10, 375. https://doi.org/10.3390/vetsci10060375
Guercio A, Mira F, Di Bella S, Gucciardi F, Lastra A, Purpari G, Castronovo C, Pennisi M, Di Marco Lo Presti V, Rizzo M, et al. Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo). Veterinary Sciences. 2023; 10(6):375. https://doi.org/10.3390/vetsci10060375
Chicago/Turabian StyleGuercio, Annalisa, Francesco Mira, Santina Di Bella, Francesca Gucciardi, Antonio Lastra, Giuseppa Purpari, Calogero Castronovo, Melissa Pennisi, Vincenzo Di Marco Lo Presti, Maria Rizzo, and et al. 2023. "Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo)" Veterinary Sciences 10, no. 6: 375. https://doi.org/10.3390/vetsci10060375
APA StyleGuercio, A., Mira, F., Di Bella, S., Gucciardi, F., Lastra, A., Purpari, G., Castronovo, C., Pennisi, M., Di Marco Lo Presti, V., Rizzo, M., & Giudice, E. (2023). Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (Mustela putorius furo). Veterinary Sciences, 10(6), 375. https://doi.org/10.3390/vetsci10060375