16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
3. Results
3.1. Bacterial Concentration Results
3.2. Sequence Processing
3.3. Four Probiotics Affect Gut Microbiota Diversity in Mice
3.4. Four Probiotics Alter the Distribution of Gut Microbiota in Mice
3.5. α-Diversity Analysis
3.6. β-Diversity Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number. | Strain | Source | Preservation Location |
---|---|---|---|
1 | L. plantarum | Separated from mink manure in August 2017 | Laboratory of Veterinary Microbiology and Immunology, College of Veterinary Medicine, Qingdao Agricultural University |
2 | L. acidophilus | Separated from mink manure in August 2017 | Laboratory of Veterinary Microbiology and Immunology, College of Veterinary Medicine, Qingdao Agricultural University |
3 | B. subtilis | Separated from mink manure in August 2017 | Laboratory of Veterinary Microbiology and Immunology, College of Veterinary Medicine, Qingdao Agricultural University |
4 | E.faecalis | Separated from mink manure in August 2017 | Laboratory of Veterinary Microbiology and Immunology, College of Veterinary Medicine, Qingdao Agricultural University |
Time | 6 h | 8 h | 10 h | 12 h | 14 h | |
---|---|---|---|---|---|---|
Strains | ||||||
L. plantarum | 6.01 × 108 | 1.72 × 109 | 2.34 × 109 | 2.58 × 109 | 2.62 × 109 | |
L. acidophilus | 2.98 × 108 | 1.35 × 109 | 2.35 × 109 | 3.55 × 109 | 3.59 × 109 | |
B. subtilis | 1.78 × 109 | 2.28 × 109 | 2.62 × 109 | 2.65 × 109 | 2.66 × 109 | |
E.faecalis | 2.20 × 109 | 2.61 × 109 | 2.82 × 109 | 2.90 × 109 | 2.91 × 109 |
Sample ID | Input | Filtered | Denoised | Merged | Non-Chimeric | Non-Singleton |
---|---|---|---|---|---|---|
La | 77,695 | 71,285 | 69,469 | 63,712 | 44,755 | 42,656 |
Lp | 132,321 | 122,785 | 119,571 | 109,512 | 72,120 | 70,366 |
MRS | 57,511 | 53,107 | 51,734 | 47,739 | 34,229 | 32,166 |
Bs | 57,891 | 53,649 | 52,800 | 50,650 | 36,812 | 35,819 |
Ef | 56,746 | 52,825 | 51,550 | 47,408 | 36,800 | 36,000 |
LB | 77,804 | 73,483 | 72,382 | 69,136 | 49,334 | 48,793 |
Sample | Chao1 | Shannon | Simpson |
---|---|---|---|
La | 3555.3 | 8.06856 | 0.958022 |
Lp | 3532.69 | 7.7823 | 0.961972 |
MRS | 2582.61 | 8.39776 | 0.964692 |
Bs | 1839.56 | 6.62015 | 0.88648 |
Ef | 1512.04 | 6.2814 | 0.904921 |
LB | 1214.77 | 6.23755 | 0.901051 |
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Ren, J.; He, F.; Yu, D.; Xu, H.; Li, N.; Cao, Z.; Wen, J. 16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice. Vet. Sci. 2023, 10, 288. https://doi.org/10.3390/vetsci10040288
Ren J, He F, Yu D, Xu H, Li N, Cao Z, Wen J. 16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice. Veterinary Sciences. 2023; 10(4):288. https://doi.org/10.3390/vetsci10040288
Chicago/Turabian StyleRen, Jianwei, Fang He, Detao Yu, Hang Xu, Nianfeng Li, Zhi Cao, and Jianxin Wen. 2023. "16S rRNA Gene Amplicon Sequencing of Gut Microbiota Affected by Four Probiotic Strains in Mice" Veterinary Sciences 10, no. 4: 288. https://doi.org/10.3390/vetsci10040288