Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients
2.2. Inclusion/Exclusion Criteria
2.3. Sampling and Patients’ Management
2.4. 2-DE and Mass Spectrometry Analysis
2.5. Statistical Analysis
3. Results
Fecal Proteomics
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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N. | Breed | Age (Years) | Sex | Therapy Undertaken | FS § T0 | FS § T1 | FS § T2 | * Serum Albumin (g/dL) [Reference Value 2.60–3.30] |
---|---|---|---|---|---|---|---|---|
1 | English Setter | 2 | M | Diet ** + probiotics | 4.5 | 5 | 2 | 2.55 |
2 | Pinscher | 4 | M | Diet ** + probiotics + ranitidine + maropitant + complementary feed to support liver function | 3.5 | 4 | 2.5 | 3.56 |
3 | Cocker Spaniel | 3 | M | Probiotics + fluids + ranitidine + maropitant | 3 | - | - | 2.67 |
4 | Labrador Retriever | 2 | F | Probiotics + ranitidine + metoclopramide | 4 | 3 | 2 | 3.7 |
5 | Labrador Retriever | 7 | F | Diet ** + fluids + probiotics + ranitidine | 3 | 3 | 2 | 2.7 |
6 | Australian Shepherd | 5 | M | Diet ** + probiotics | 5 | 4.5 | 2 | - |
7 | Border Collie | 9 | F | Diet ** + probiotics | 4 | 3 | 2 | - |
8 | Mixed breed | 10 | M | Diet ** + probiotics | 4 | 1 | 1 | - |
9 | Mestizo Pinscher | 2 | F | Diet ** + probiotics | 3 | 2 | 2 | 2.86 |
Mean values | 3.8 | 3.2 | 1.9 | 3.00 |
Spot ID a Healthy | Spot ID b AUD | Protein c | Score d | Mr (kDa)/pI e | Mr (kDa)/pI f | Sequence |
---|---|---|---|---|---|---|
n.d. | B | Albumin [Canis lupus familiaris] | 39 | 68.5/5.52 | 35.7/4.8 | DFAEISK |
present | Y | Serum albumin isoform X1 [Canis lupus familiaris] | 56 | 68.6/5.51 | 72/5.8 | LVAAAQAALV |
present | Y2 | Serum albumin isoform X1 [Canis lupus familiaris] | 41 | 68.6/5.51 | 63/5.8 | ADFAEISK |
n.d. | E1 | Albumin [Canis lupus familiaris] | 216 | 68.5/5.52 | 53.3/5.3 | DFAEISKVVTDLTK |
n.d. | E2 | Albumin [Canis lupus familiaris] | 104 | 68.5/5.52 | 50.7/5.2 | KLGEYGFQNALLVR |
n.d. | L1 | Intestinal-type alkaline phosphatase [Mus musculus] | 103 | 60.2/6.24 | 52.3/7.2 | NLIIFLGDGMGVPT |
n.d. | L2 | Albumin [Canis lupus familiaris] | 131 | 68.5/5.52 | 36.1/6.1 | LVAAAQAALV |
n.d. | L3 | Albumin [Canis lupus familiaris] | 332 | 68.5/5.52 | 33.8/5.8 | LVAAAQAALV |
n.d. | L4 | Albumin [Canis lupus familiaris] | 131 | 68.5/5.52 | 43.8/5.5 | LVAAAQAALV |
n.d. | L6 | Albumin [Canis lupus familiaris] | 100 | 68.5/5.52 | 29.9/5.3 | DFAEISKVVT |
present | V1 | Alkaline phosphatase [Canis lupus familiaris] | 125 | 68.6/6,47 | 59/6.6 | ANYQTIGVSAAAR |
present | V2 | Alkaline phosphatase [Canis lupus familiaris] | 117 | 48.3/6.15 | 58/6.6 | ANYQTIGVSAAAR |
present | H | Chymotrypsin-C-like [Canis lupus dingo] | 49 | 29.1/5.33 | 29/5.6 | LAEPVQLSDTIK |
present | H1 | Elastase-3B, Proteinase E [Canis lupus familiaris] | 40 | 28.8/5.27 | 29/5.2 | VSAFNDWIEEVMSS |
present | H3 | Immunoglobulin kappa light chain [Felis catus] | 41 | 26.7/6.10 | 29/6.3 | FSGSGSGTDFTLR |
present | G | Immunoglobulin λ-1 light chain [Canis lupus familiaris] | 34 | 25.2/6.88 | 29/7.1 | KGTHVTVLGQPK |
present | G1 | Immunoglobulin λ-1 light chain [Felis catus] | 39 | 27.8/8.17 | 29/7.6 | QSNNKYAASSYL |
present | G2 | Immunoglobulin λ-light chain VLJ region [Homo sapiens] | 42 | 29.0/8.14 | 29/6.6 | EFGGGTKLTVLGQP |
present | G3 | Immunoglobulin λ-light chain VLJ region [Homo sapiens] | 30 | 29.0/8.14 | 27/8.4 | EFGGGTKLTVLGQP |
present | G4 | Immunoglobulin λ-light chain VLJ region [Homo sapiens] | 40 | 29.0/8.14 | 27.7/8.9 | QSNNKYAASSYL |
n.d. | G5 | Immunoglobulin kappa chain V region [Canis lupus familiaris] | 84 | 12.0/6.4 | 25/7 | FSGSGSGTDFTLR |
present | F1 | Nuclear pore membrane glycoprotein 210 [Canis lupus familiaris] | 29 | 192.4/6.30 | 19.6 ± 1.5/5.8 ± 0.14 | TALLVTASISGSHAPR |
present | F2 | Cytosol aminopeptidase [Canis lupus familiaris] | 29 | 56.2/8.03 | 21.0 ± 1.3/5.7 ± 0.07 | EILNISGPPLK |
n.d. | F3 | Immunoglobulin lambda variable 4–60 [Homo sapiens] | 69 | 12.9/5.8 | 16.3 ± 1.6/6 ± 0.1 | FSGSSSGADR |
n.d. | W1 | Immunoglobulin J chain [Homo sapiens] | 43 | 18.1/5.12 | 21 ± 0.07/4.1 ± 0.05 | IIVPLNNR |
n.d. | Z4 | Albumin [Canis lupus familiaris] | 152 | 68.5/5.52 | 8.8 ± 0.5/4.1 ± 0.03 | LVAAAQAALV |
n.d. | Z5 | Fatty acid-binding protein, intestinal [Homo sapiens] | 69 | 15.2/6.6 | 8.3 ± 3.4/5.9 ± 0.07 | LTITQEGNK |
n.d. | Z6 | Albumin [Canis lupus familiaris] | 76 | 68.5/5.52 | 7.1 ± 3.1/5.5 ± 0.2 | EAYKSEIAHRYNDLGE |
n.d. | Z8 | Albumin [Canis lupus familiaris] | 80 | 68.5/5.52 | 8.1 ± 0.1/6 ± 0.1 | EAYKSEIAHRYNDLGE |
n.d. | Z10 | Superoxide dismutase [Cu-Zn] [Canis lupus familiaris] | 233 | 15.9/5.7 | 8.8 ± 0.5/6.2 ± 0.1 | EKRDDLGKGDNEEST |
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Cerquetella, M.; Mangiaterra, S.; Rossi, G.; Gavazza, A.; Marchegiani, A.; Sagratini, G.; Ricciutelli, M.; Angeloni, S.; Fioretti, L.; Marini, C.; et al. Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment. Vet. Sci. 2023, 10, 233. https://doi.org/10.3390/vetsci10030233
Cerquetella M, Mangiaterra S, Rossi G, Gavazza A, Marchegiani A, Sagratini G, Ricciutelli M, Angeloni S, Fioretti L, Marini C, et al. Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment. Veterinary Sciences. 2023; 10(3):233. https://doi.org/10.3390/vetsci10030233
Chicago/Turabian StyleCerquetella, Matteo, Sara Mangiaterra, Giacomo Rossi, Alessandra Gavazza, Andrea Marchegiani, Gianni Sagratini, Massimo Ricciutelli, Simone Angeloni, Licia Fioretti, Carlotta Marini, and et al. 2023. "Fecal Protein Profile in Eight Dogs Suffering from Acute Uncomplicated Diarrhea before and after Treatment" Veterinary Sciences 10, no. 3: 233. https://doi.org/10.3390/vetsci10030233