1. General Introduction
Red fruit juices have long been popular in Europe where the technology has been extensively developed for high value added fruits [
1]. The documented phytochemical composition of red fruits is resulting in renewed global interest in industrial demand for these juices [
2], since many red fruit juices, especially those that are naturally colorful, have high levels of antioxidants. Several researchers have concentrated their work on studying the formation of consumers’ food quality expectations and sensorial experiences, as determinants of food acceptance [
3,
4]. Health issues can lead to the choice of red fruit juices and consequently to their acceptance and consumption [
5]. Common fruits, such as berries, grapes, pomegranate, guava, sweetsop, persimmon and plum are rich in antioxidant phytochemicals [
6,
7,
8] that have a protective role associated with their antioxidant activity, since overproduction of oxidants (reactive oxygen species and reactive nitrogen species) in the human body is involved in the pathogenesis of many chronic diseases [
9], for instance, cardiovascular diseases, diabetes and cancers. It has been demonstrated that red-fruit juice grains wield a protective effect against the development of these diseases [
10,
11,
12]. However, economic considerations limit but do not eliminate 100 percent red fruit products. The characteristic dominating flavor and color of these red fruits make them ideal for blending [
13].
2. Red Fruit Juices
The market for non-alcoholic beverages is growing because consumers prefer healthier beverages. Nowadays, red fruit juices are known for their health benefits due to their higher phenolic compounds concentration, namely in flavonoid and non-flavonoid compounds, and consequently high antioxidant activity. The main flavonoid compounds in red fruits are anthocyanins (cyanidin and other colorings of the skin, for example in red grape, delphinidin, peonidin, malvidin, pelargonidin, petunidin), flavanols (proanthocyanidins and catechins), flavonols (derivatives of rutin, quercetin, myricetin and kaempferol). For non-flavonoid compounds, the main phenolic acids in red fruits are represented by hydroxylated derivatives of benzoic and hydroxycinnamic acid [
14,
15].
Within the red fruits used for the production of juices, pomegranate, red currant, blood orange, black currant, cherry, red raspberry, blackberry, strawberry, blueberry, elderberry and grape berries are amongst the richest in anthocyanins—water soluble pigments that contribute to the blue, purple, and red color in many fruits and their high antioxidant activity [
16]. Numerous studies have shown that anthocyanins present a wide range of biological activities including antioxidant, anti-inflammatory, antimicrobial and anti-carcinogenic activities [
17,
18,
19]. So, a high correlation has been shown between antioxidant activity and total anthocyanin concentration of red fruit juices [
20]. However, anthocyanin stability depends on the existence of methoxyl or a hydroxyl group in the anthocyanin structure, giving them more or less stability and thus having an impact on their bioavailability. For example, delphinidin-3-glucoside has three hydroxyl groups in its B ring, being the most unstable anthocyanin, while malvidin-3-glucoside with more methoxyl groups is the most stable anthocyanin. Consequently, the biological activity of anthocyanins can also be influenced by glycosylation, which makes the anthocyanin more water soluble but less reactive to free radicals, subsequently decreasing their antioxidant activity [
21].
For example, there is a motivation to produce red fruit beverages like grape juice or grape nectar for their important nutritional properties [
22], such as antioxidant activity, due to the presence of phenolic compounds [
23]. Grape juice is an unfermented beverage obtained from the dilution of grape pulp in water, with or without addition of sugars and acids [
24]. Nevertheless, the existing juice processing technology leads to significant losses of these compounds through heating degradation and poor extraction from fruit. Therefore, consumers demand fresh, unpasteurized juices. According to Yuste et al. [
25], in order to better preserve the properties of red fresh juices, research into alternative juice processing technology has been stimulated due to consumer demand for novel, natural and fresh-like beverages that are both safe and have improved nutritional and sensory characteristics. Manea [
26] observed that the preservation of red fruit juices at refrigeration temperature conserves anthocyanins and tannins. Elderberry juice presented high concentration of anthocyanins, 0.84 g/L and tannins 3.22 g/L. Elderberry and grapes have anthocyanins in the skin, however the ratio of the skin and pulp is lower in elderberry, consequently the elderberry juice will be more concentrated in anthocyanins than grape juice (0.45 g/L). After three months of storage at refrigeration temperature, anthocyanin content decreased by 49.74% for grape juice and by 25.15% for the elderberry juice, since in the elderberry juice tannins were better preserved and act as antioxidants and preservatives, inhibiting juice enzymes that contributed to the protection of the anthocyanins; therefore juice color and flavor were better maintained [
26]. So, novel treatments such as high hydrostatic pressure, high-intensity pulsed electric fields, and ultrasound are alternative treatments to pasteurization, in order to avoid the detrimental effects produced by high temperatures in the degradation of phenolic compounds as well as to be able to maintain the sensorial quality attributes of the final product [
27,
28].
3. Composition of Red Fruit Juices
Grape nectar and beverages presented a different physicochemical composition to those from the grape juice, since they were obtained from a dilution of grape juice. Among the compounds that showed greater concentrations in grape juice are total soluble solids (16.2 °Brix), phenolic compounds, such as total polyphenol index (65.8), anthocyanins (283.7 mg/L) and color intensity (1.245), organic acids, namely tartaric acid (6.7 g/L) and malic acid (3.3 g/L). Grape nectar and beverage are characterized by a higher °Brix/titratable ratio, respectively 29.7 and 26.7 [
23].
Red fruit juices’ phenolic composition, namely total phenolic compounds and total anthocyanins ranged from 1234.27 mg GAE/L to 6361.89 mg GAE/L, for red raspberry and elderberry, respectively, and the total anthocyanins ranged from 205.98 mgCGE/L to 4188.63 mgCGE/L, for strawberry and elderberry, respectively [
20]. Total anthocyanins were the main phenolic compounds presented in elderberry juice (66%) or black currant juice (56%) [
20].
The antioxidant activity of red fruit juices ranged from 4.07 to 62.14 μmol TE/mL for sweet cherry and elderberry, respectively [
20], elderberry juice being the ones that have higher concentration in total phenolic compounds, total anthocyanins and consequently higher antioxidant activity. Red fruit juices showed considerable variations in anthocyanin content and profile as shown in
Table 1. Some red fruit juices have low concentration of anthocyanins, such as strawberry, red raspberry and sweet cherry juice, while the concentration of these flavonoid compounds is high in elderberry and black currant juice.
Monomeric anthocyanins existing in red fruit juices were derivatives of cyanidin, delphinidin, pelargonidin, malvidin and peonidin. So, it is evident that juices produced with different red fruits, such as strawberry, raspberry, blueberry, black currant and grapes, differ in the quantity and type of anthocyanins. The family of European
Vitis vinifera vines is characterized by anthocyanins, which have only one molecule of glucose, while non-vinifera vines such as
Vitis labrusca or
Vitis rotundafolia grapes and American hybrids have anthocyanins with two molecules of glucose. Obón et al. [
29], Lopes da Silva et al. [
30], Jakobek et al. [
20] and Stój et al. [
31] determined in strawberry juices the existence of cyanidin 3-glucoside, pelargonidin 3-glucoside, and pelargonidin 3-rutinoside, the major anthocyanin in this juice being pelargonidin 3-glucoside (
Table 1). The anthocyanin profile from red raspberry juices showed five anthocyanins, namely cyanidin 3-sophoroside, cyanidin 3-glucosyl-rutinoside, cyanidin 3-glucoside, pelargonidin 3-sophoroside, and cyanidin 3-rutinoside [
29,
31,
32]. Blackcurrant juices are characterized by monomeric anthocyanins such as delphinidin 3-glucoside, delphinidin 3-rutinoside, cyanidin 3-glucoside, cyanidin 3-rutinoside, and delphinidin 3-rutinoside [
29,
32,
33,
34]. Pelargonidin-3-glucoside and cyanidin-3-xylorutinoside were the main anthocyanins in strawberry and red currant juices, respectively. Those anthocyanins were not detected in raspberry and black currant juices, in which cyanidin-3-sophoroside as well as delphinidin-3-rutinoside and cyanidin-3-rutinoside were the main anthocyanins, respectively [
31]. Blueberry juices anthocyanin profile studied by several authors [
29,
32,
35,
36,
37], showed fourteen different anthocyanins, namely dephinidin 3-galactoside, delphinidin 3-glucoside, cyanidin 3-galactoside, delphinidin 3-arabinoside, cyanidin 3-glucoside, petunidin 3-galactoside, cyanidin 3-arabinoside, petunidin 3-glucoside, peonidin 3-galactoside, petunidin 3-arabinoside, peonidin 3-glucoside, malvidin 3-galactoside, malvidin 3-glucoside, and malvidin 3-arabinoside (
Table 1). Grape juice anthocyanins are mainly delphinidin 3-glucoside, cyanidin 3-glucoside, petunidin 3-glucoside, peonidin 3-glucoside and malvidin 3-glucoside [
32,
37]. According to Jakobek et al. [
20], elderberry, blackberry and sour cherry juice are characterized by cyanidin derivatives, whereas black currant juice by delphinidin and cyanidin derivatives and strawberry juice are characterized by pelargonidin derivatives.
Table 1 showed that each red fruit juice possesses a unique anthocyanin profile. Although all red fruit juices are good sources of bioactive phytochemicals, elderberry and black currant juices stand out in higher anthocyanin concentration and consequently have higher antioxidant activities [
20].
4. Color and Flavor Perception vs. Consumer Acceptance
There is no color without light, nor is there color perception without a sensory organ and brain to process visual input. Our visual perceptions are initiated and influenced by the anatomical structure of the eye. The cornea and lens, on the eye, act together like a camera lens to focus an image of the visual world on the retina, at the back of the eye, which acts like the film of a camera. An image is formed, upside down, on the retina—a structure less than 0.5 mm thick (
Figure 1) [
38,
39]—with light rays converging most at the cornea and upon entering and exiting the lens [
40].
The human retina contains two types of photoreceptors, rods and cones. Cones are involved in color vision, while rods are stimulated by weak light like moonlight. The photoreceptors synapse on layer upon layer of interneurons that are innervated by different combinations of photoreceptor cells. All these signals are processed and interpreted by the part of the brain called the visual cortex [
41].
Color is the single most important product-intrinsic sensory cue when it comes to setting people’s expectations regarding the likely taste and flavor of food and drink. The color of food is an often ignored sensory attribute that can really change consumers’ aroma/flavor perception. A growing body of scientific research now suggests that our experience of taste and flavor is determined to a large degree by the expectations that we generate (often automatically) prior to tasting [
43]. Under most everyday conditions, consumers have the opportunity to inspect food and drink visually before deciding on whether or not to buy or taste it. As Roth et al. [
44] mentioned in their work, when food colors are different from the expected norm, flavor identification is decreased, the color-flavor association becomes stronger, and color has a greater effect on liking of the food [
44]. Consumers associate certain colors with certain flavors, so it is important for food manufacturers to have the expected color in their food. Color and appearance of food create expectations that affect how we feel and behave [
45], and influence food identification [
46]. This occurs in simple foods and in complex foods in which there are multiple taste stimuli. For example, when white wine is colored red, the tendency is to describe the wine using red wine odor terms instead of the white wine odor terms that are instinctively used in white wine [
47]. As such, food color can be considered the most important product-intrinsic sensory cue leading the sensory and hedonic expectations that the consumer holds concerning the foods and drinks that they search for, purchase, and that they may subsequently consume [
48].
Therefore, it is not surprising that the colors sector of the food industry has become very important. Color producing companies are high-tech and sophisticated and their main objective is naturalness. Besides the juice industry, red fruit juices have also been used to enhance or change the color of different food recipes [
49]. According to this author, red fruit juices behave in different circumstances. In mixtures of water, soy milk and yoghurt, each made with 10% juice, it was noticed that each juice changed color as a reaction to such things as pH or presence of proteins. Anthocyanins can change from bright red to blue depending on the pH (
Figure 2) [
49].
The best juices to use as coloring agents are blackcurrant (
Ribes nigrum), elderberry (
Sambucus niger) and aronia (
Aronia melanocarpa) (
Figure 2). However, the taste of blackcurrant is very strong and, as such, elderberry and aronia are used in cases where fruit flavor would be not required [
49].
Consumers often confuse tastes with flavors. Taste perception refers to those sensations that are elicited by the stimulation of the gustatory receptors on the tongue—sweet, sour, salty, bitter, umami and fat taste [
50]. Humans, usually, never experience pure tastants in isolation. We mostly experience flavors, resulting from the combination of taste, retronasal olfaction (referred to as ‘mouth smell’, in contrast to orthonasal olfaction or ‘sniffing’,
Figure 3) and trigeminal inputs. ‘fruity’, ‘meaty’, ‘floral’ and ‘burnt’ are all flavor descriptors [
51].
Smell (both orthonasal and retronasal) often combines with taste to enhance our perception of flavor [
51]. A study performed by Dalton et al. [
52] demonstrated that the ability to detect threshold levels of benzaldehyde (almond-like aroma) sniffed in solution could be intensely enhanced by placing a subthreshold drop of saccharin on the tongue.
One important aspect of food consumption that, sadly, is often over-looked, is that consumers want to enjoy their food. In fact, many foods are consumed almost entirely for the pleasure value they impart [
53]. Since childhood, our behavior towards foods seems to be strongly influenced by the effects of taste and flavor. Steiner [
54], in 1979, studied the facial expressions of young babies when given solutions of sweet and bitter compounds (
Figure 4) the infant reacted in very different ways. Sweet tastes elicited a facial acceptance response, but, bitter tastes, gave a very different response, with tight closing of the eyes, gaping mouth and sudden turn of the head [
54]. These inborn reactions of young babies are indicative of a basic survival instinct in all of us. Some of these early pre-dispositions tend to remain with us throughout life. We all like sweets and pleasant smells! However, some other food preferences (liking for ‘bitter’ tasting lagers or hot spicy foods) demonstrate a degree of acquired liking [
53].
Studies have demonstrated that changing the color (both the hue and the intensity) can change the perceived flavor and the intensity of the taste [
55]. Thus, the flavor perception in food is influenced by color. Red food coloring also appears to be a particularly good inducer of sweetness. It seems that we may have internalized the environmental association between sweetness and redness in ripe fruits [
51].
Stevenson [
56] has demonstrated that whether or not a given color, added to a food or beverage, affects a person’s taste/flavor perception largely depends on the meaning that the person associates with foods and drinks having that color. In an experiment conducted in UK among young consumers, it was shown that they expected that a drink colored blue would have a raspberry-like taste/flavor. Taiwanese young consumers expect that such a drink would present a mint taste/flavor [
56]. Therefore, one needs to know what expectations a consumer has in order to predict what it is they will likely experience when a particular color is added to a given food or drink [
51]. If the expectation and the experience are not too different from one another, for instance, expecting blackcurrant, while getting blackberry, then the consumer will likely report experiencing the ‘expected’ flavor [
51].
Despite growing demand by consumers for healthy beverages, artificial colors are still widely used. Red drinks’ labels generally conformed to the requirements of the law, but food product labels can often be misleading to consumers about the real characteristics of the product. Fallico et al. [
57] determined by HPLC with UV-Vis detection, the anthocyanins levels of, and artificial colors in, red orange juices and other red beverages. They found that red juice-based drinks, containing from 0% to 30% of red orange, berry, grape or pomegranate juices, had low levels of anthocyanins (7 mg/L) and high levels of E129 (Allura Red AC, a red azo dye) (32 mg/L), which were responsible for about 90.7% of the color of these beverages. Red health drinks, enriched with vitamins and polyphenols, contained from 3% to 50% of red fruit juices, but, in this case, the E129 levels were also higher than anthocyanins (about 22 mg/L and 9 mg/L, respectively), and were responsible for 76.1% of the beverages’ color. They found no correlation between the level of anthocyanins and the declared percentage of red fruits, although all of the beverages claimed to contain red fruits on the labels.
6. Taste and Flavor Modification Techniques
Blackcurrant juices are often sweetened with sugars or other added sweeteners to reduce sourness and astringency [
83]. However, addition of sugars compromises the health effects of the juices, making it less attractive for consumers with health issues. Diluting the juice with water and mixing with other fruits are alternative ways often applied to improve the sensory properties of the final product. Optimal selection of cultivars and processing techniques could minimize the need of dilution, sweetening and other types of modification [
84].
Laaksonen and co-workers [
84] compared the composition and sensory properties of juices extracted, at laboratory scale, from berries of different blackcurrant cultivars by different processes. The cultivars used were Mortti, Mikael, Marski, Ola and a new breed no. 15 (Breed15) of blackcurrant cultivated in Southern Finland. Two processing methods were applied to press juices from berries of each cultivar: a non-enzymatic pressing and an enzyme-aided juice pressing. Both juice-processing technologies and cultivars significantly affect the chemical composition and sensory quality of blackcurrant juice. Enzyme-aided juice pressing led to increased juice yield, with higher content of phenolic compounds increasing the intensities of astringency and bitterness and adversely affecting the sensory properties of the juices when compared with non-enzyme pressing [
84]. Therefore, non-enzyme pressing provides an alternative processing technology to produce juices of more pleasant sensory profiles. Moreover, non-enzymatic processing can be considered as an alternative processing technology to produce more natural products, including juices with more pleasant sensory profiles. Non-enzyme pressing reduces the need of modification (dilution and blending) of the end products with less addition of sugars or other sweeteners. This process may also be used to produce raw material for non-juice products such as purees and smoothies. Due to the low yield, efficient use of non-enzymatic juice processing requires innovative exploitation methods for press residues [
84].
Development and production of natural berry products with the ideal sensory properties for various consumer groups still remains a challenge for the food industry. Recently Laaksonen et al. [
85], in a study with the blackcurrant cultivar ‘Mortti’, found that the press residue from the non-enzyme-aided pressing can be further pressed by an enzyme-aided process to produce polyphenol-rich juice with a fermented, juice-type aroma profile that is well accepted by the consumers. The new juice concepts studied after process optimization could be commercialized, providing alternative products for various consumer groups. The processes may be extended to the processing of other berries to increase the consumption of berries as a part of healthy diet.