Unraveling the Mechanism of Platelet Aggregation Suppression by Monoterpenoids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemistry
2.1.1. Synthesis of 2-((((1S,5R)-6,6-Dimethylbicyclo[3.1.1]hept-2-en-2-yl)methyl)thio)ethan-1-ol (4)
2.1.2. Synthesis of 2-((((1S,5R)-6,6-Dimethylbicyclo[3.1.1]hept-2-en-2-yl)methyl)amino)ethan-1-ol (5)
2.2. Platelet Aggregation Assays
2.3. Molecular Docking
2.4. NMR Experiments
2.5. Cells Viability MTS Assay
2.6. Statistic Analysis
3. Results
3.1. Synthesis of Monoterpenoids and Their Effect on Platelet aggregation
3.2. Molecular Docking
3.3. NMR Experiments
3.4. Cytotoxicity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Inductor | Solely Inductor | 3 | 4 | 5 |
---|---|---|---|---|
ADP | 66.5 ± 2.4 | 34.2 ± 1.2 * | 30.4 ± 3.2 * | 23.4 ± 2.8 * |
adrenaline | 53.8 ± 2.2 | 8.4 ± 3.8 * | 10.2 ± 2.6 * | 2.6 ± 1.8 * |
arachidonic acid | 61.6 ± 1.4 | 0 * | 0 * | 0 * |
collagen | 70.4 ± 4.2 | 11.6 ± 3.4 * | 4.4 ± 2.8 * | 0 * |
ristocetin | 72.7 ± 3.2 | 30.2 ± 2.4 * | 20.8 ± 5.2 * | 42.4 ± 3.8 * |
Compound | Amino Acid Residues |
---|---|
3 | ARG122, LYS125, THR126, ARG128, PRO129, PHE130, LYS131, THR132, LYS232, LYS233, VAL238 |
4 | ARG122, LYS125, THR126, ARG128, PRO129, PHE130, LYS131, THR132, LYS233, VAL234, VAL238, LEU301 |
5 | ASP1005, GLU1008, THR1009, ASP1012, ASN1013, VAL1016, LYS1032 |
Compound | IC50 Value, µM |
---|---|
3 | 548 ± 52.7 |
4 | 314 ± 46.5 |
5 | 450 ± 71.6 |
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Nikitina, L.E.; Pavelyev, R.S.; Gilfanov, I.R.; Kiselev, S.V.; Azizova, Z.R.; Ksenofontov, A.A.; Bocharov, P.S.; Antina, E.V.; Klochkov, V.V.; Timerova, A.F.; et al. Unraveling the Mechanism of Platelet Aggregation Suppression by Monoterpenoids. Bioengineering 2022, 9, 24. https://doi.org/10.3390/bioengineering9010024
Nikitina LE, Pavelyev RS, Gilfanov IR, Kiselev SV, Azizova ZR, Ksenofontov AA, Bocharov PS, Antina EV, Klochkov VV, Timerova AF, et al. Unraveling the Mechanism of Platelet Aggregation Suppression by Monoterpenoids. Bioengineering. 2022; 9(1):24. https://doi.org/10.3390/bioengineering9010024
Chicago/Turabian StyleNikitina, Liliya E., Roman S. Pavelyev, Ilmir R. Gilfanov, Sergei V. Kiselev, Zulfiya R. Azizova, Alexander A. Ksenofontov, Pavel S. Bocharov, Elena V. Antina, Vladimir V. Klochkov, Ayzira F. Timerova, and et al. 2022. "Unraveling the Mechanism of Platelet Aggregation Suppression by Monoterpenoids" Bioengineering 9, no. 1: 24. https://doi.org/10.3390/bioengineering9010024