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Article

Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro

1
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA
2
Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX 75080, USA
3
Department of Biological Sciences, University of Texas at Dallas, Richardson, TX 75080, USA
*
Author to whom correspondence should be addressed.
Bioengineering 2020, 7(2), 44; https://doi.org/10.3390/bioengineering7020044
Received: 29 March 2020 / Revised: 8 May 2020 / Accepted: 12 May 2020 / Published: 16 May 2020
Sensory neurons respond to noxious stimuli by relaying information from the periphery to the central nervous system via action potentials driven by voltage-gated sodium channels, specifically Nav1.7 and Nav1.8. These channels play a key role in the manifestation of inflammatory pain. The ability to screen compounds that modulate voltage-gated sodium channels using cell-based assays assumes that key channels present in vivo is maintained in vitro. Prior electrophysiological work in vitro utilized acutely dissociated tissues, however, maintaining this preparation for long periods is difficult. A potential alternative involves multi-electrode arrays which permit long-term measurements of neural spike activity and are well suited for assessing persistent sensitization consistent with chronic pain. Here, we demonstrate that the addition of two inflammatory mediators associated with chronic inflammatory pain, nerve growth factor (NGF) and interleukin-6 (IL-6), to adult DRG neurons increases their firing rates on multi-electrode arrays in vitro. Nav1.7 and Nav1.8 proteins are readily detected in cultured neurons and contribute to evoked activity. The blockade of both Nav1.7 and Nav1.8, has a profound impact on thermally evoked firing after treatment with IL-6 and NGF. This work underscores the utility of multi-electrode arrays for pharmacological studies of sensory neurons and may facilitate the discovery and mechanistic analyses of anti-nociceptive compounds. View Full-Text
Keywords: inflammation; dorsal root ganglion; nociceptor; IL-6; NGF; Nav1.7; Nav1.8; microelectrode arrays inflammation; dorsal root ganglion; nociceptor; IL-6; NGF; Nav1.7; Nav1.8; microelectrode arrays
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MDPI and ACS Style

Atmaramani, R.R.; Black, B.J.; de la Peña, J.B.; Campbell, Z.T.; Pancrazio, J.J. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering 2020, 7, 44. https://doi.org/10.3390/bioengineering7020044

AMA Style

Atmaramani RR, Black BJ, de la Peña JB, Campbell ZT, Pancrazio JJ. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering. 2020; 7(2):44. https://doi.org/10.3390/bioengineering7020044

Chicago/Turabian Style

Atmaramani, Rahul R., Bryan J. Black, June Bryan de la Peña, Zachary T. Campbell, and Joseph J. Pancrazio. 2020. "Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro" Bioengineering 7, no. 2: 44. https://doi.org/10.3390/bioengineering7020044

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