Next Article in Journal
Bioengineering of rFVIIa Biopharmaceutical and Structure Characterization for Biosimilarity Assessment
Next Article in Special Issue
Effects of Heterologous tRNA Modifications on the Production of Proteins Containing Noncanonical Amino Acids
Previous Article in Journal
Acknowledgement to Reviewers of Bioengineering in 2017
Previous Article in Special Issue
Chitosan–Cellulose Multifunctional Hydrogel Beads: Design, Characterization and Evaluation of Cytocompatibility with Breast Adenocarcinoma and Osteoblast Cells
Open AccessArticle

Development of Self-Assembled Nanoribbon Bound Peptide-Polyaniline Composite Scaffolds and Their Interactions with Neural Cortical Cells

Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, New York, NY 10458, USA
Author to whom correspondence should be addressed.
Academic Editor: Gary Chinga Carrasco
Bioengineering 2018, 5(1), 6;
Received: 4 November 2017 / Revised: 6 January 2018 / Accepted: 8 January 2018 / Published: 13 January 2018
(This article belongs to the Special Issue Novel Biocomposite Engineering and Bio-Applications)
Degenerative neurological disorders and traumatic brain injuries cause significant damage to quality of life and often impact survival. As a result, novel treatments are necessary that can allow for the regeneration of neural tissue. In this work, a new biomimetic scaffold was designed with potential for applications in neural tissue regeneration. To develop the scaffold, we first prepared a new bolaamphiphile that was capable of undergoing self-assembly into nanoribbons at pH 7. Those nanoribbons were then utilized as templates for conjugation with specific proteins known to play a critical role in neural tissue growth. The template (Ile-TMG-Ile) was prepared by conjugating tetramethyleneglutaric acid with isoleucine and the ability of the bolaamphiphile to self-assemble was probed at a pH range of 4 through 9. The nanoribbons formed under neutral conditions were then functionalized step-wise with the basement membrane protein laminin, the neurotropic factor artemin and Type IV collagen. The conductive polymer polyaniline (PANI) was then incorporated through electrostatic and π–π stacking interactions to the scaffold to impart electrical properties. Distinct morphology changes were observed upon conjugation with each layer, which was also accompanied by an increase in Young’s Modulus as well as surface roughness. The Young’s Modulus of the dried PANI-bound biocomposite scaffolds was found to be 5.5 GPa, indicating the mechanical strength of the scaffold. Thermal phase changes studied indicated broad endothermic peaks upon incorporation of the proteins which were diminished upon binding with PANI. The scaffolds also exhibited in vitro biodegradable behavior over a period of three weeks. Furthermore, we observed cell proliferation and short neurite outgrowths in the presence of rat neural cortical cells, confirming that the scaffolds may be applicable in neural tissue regeneration. The electrochemical properties of the scaffolds were also studied by generating I-V curves by conducting cyclic voltammetry. Thus, we have developed a new biomimetic composite scaffold that may have potential applications in neural tissue regeneration. View Full-Text
Keywords: self-assembly; templates; tissue regeneration; peptide amphiphiles self-assembly; templates; tissue regeneration; peptide amphiphiles
Show Figures

Figure 1

MDPI and ACS Style

Smith, A.M.; Pajovich, H.T.; Banerjee, I.A. Development of Self-Assembled Nanoribbon Bound Peptide-Polyaniline Composite Scaffolds and Their Interactions with Neural Cortical Cells. Bioengineering 2018, 5, 6.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop