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Bioengineering 2017, 4(3), 67; https://doi.org/10.3390/bioengineering4030067

Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells

1
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA
2
Division of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Jochen Strube
Received: 11 July 2017 / Revised: 28 July 2017 / Accepted: 29 July 2017 / Published: 2 August 2017
(This article belongs to the Special Issue Advances in Microseparation Technology)
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Abstract

Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer as a narrow band between the density-gradient medium and platelet-rich plasma. We hypothesized that microfluidic adaptation of this technique could transform this process into a rapid fractionation approach where samples are separated in a continuous fashion while being exposed to lower levels of stress (<100 g) for shorter durations of time (<3 min). To demonstrate proof-of-concept, we designed a microfluidic density-gradient centrifugation device and constructed a setup to introduce samples and medium like Ficoll in a continuous, pump-less fashion where cells and particles can be exposed to centrifugal force and separated via different outlets. Proof-of-concept studies using binary mixtures of low-density polystyrene beads (1.02 g/cm3) and high-density silicon dioxide beads (2.2 g/cm3) with Ficoll–Paque (1.06 g/cm3) show that separation is indeed feasible with >99% separation efficiency suggesting that this approach can be further adapted for separation of cells. View Full-Text
Keywords: cell separations; label-free cell separation; microfluidics; density-gradient centrifugation cell separations; label-free cell separation; microfluidics; density-gradient centrifugation
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Sun, Y.; Sethu, P. Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells. Bioengineering 2017, 4, 67.

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