Mesoporous Bioactive Glass-Incorporated Injectable Strontium-Containing Calcium Phosphate Cement Enhanced Osteoconductivity in a Critical-Sized Metaphyseal Defect in Osteoporotic Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. General Considerations
2.2. Preparation of pS100 and pS100G10
In Vitro Investigations
2.3. Animal Surgery
2.4. Sample Processing, Staining Procedures, and Histomorphometry
2.5. Immunohistochemistry
2.6. ToF-SIMS Measurements
2.7. mRNA Preparation and Gene Expression Analysis
2.8. Statistics
3. Results
3.1. In Vitro Characterization of pS100 and pS100G10
3.2. In Vivo Characterization of pS100 and pS100G10–General Information
3.3. Histomorphometry, Histology, and Immunohistochemistry
3.4. Molecular Biology
3.5. ToF-SIMS Analysis
Mineralized Bone Signals (HAP) | Collagen Signals | Strontium Signals | |||||
---|---|---|---|---|---|---|---|
m/z | Peak Label | m/z | Peak Label | m/z | Peak Label | m/z | Peak Label |
39.96 | Ca+ | 230.78 | Ca3PO5+ | 30.04 | CH4N+ | 85.89 | 86Sr+ |
55.97 | CaO+ | 286.71 | Ca4PO6+ | 44.05 | C2H6N+ | 86.91 | 87Sr+ |
56.96 | CaOH+ | 70.06 | C4H8N+ | 87.89 | Sr+ | ||
95.92 | Ca2O+ | 86.06 | C4H8NO+ | 88.90 | SrH+ | ||
102.92 | CaPO2+ | 103.90 | SrO+ | ||||
111.90 | Ca2O2+ | 104.90 | SrOH+ | ||||
112.91 | Ca2O2H+ | 150.85 | SrPO2+ | ||||
118.92 | CaPO3+ | 166.85 | SrPO3+ | ||||
134.88 | CaPO4+ | 182.86 | SrPO4+ | ||||
151.84 | Ca3O2+ | Silicon signals | 191.77 | Sr2O+ | |||
158.85 | Ca2PO3+ | m/z | Peak label | 207.80 | Sr2O2+ | ||
168.84 | Ca3O3H+ | 27.97 | Si+ | 208.80 | Sr2O2H+ | ||
174.84 | Ca2PO4+ | 28.98 | SiH+ | 254.80 | Sr2PO3+ | ||
214.79 | Ca3PO4+ | 44.98 | SiOH+ | 270.76 | Sr2PO4+ |
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Figure | 11b–d | 11e–g, 12c | 12b |
Analysis options | |||
Cycle time | 65 µs | 65 µs | 65 µs |
Analysis area | 2 × 2 mm2 | 500 × 500 µm2 | 2 × 2 mm2 |
Raster mode | sawtooth | sawtooth | sawtooth |
Primary ion current | 0.05 pA | 0.04 pA | 0.05 pA |
Pixel resolution | 1600 × 1600 | 1024 × 1024 | 2000 × 2000 |
Frames per patch | 3 | 1 | 3 |
Patch size | 0.4 mm | 0.5 mm | 0.4 mm |
Primary ion Shots/frame/pixel | 3 | 2 | 3 |
Number of scans | 10 | 150 | 15 |
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Ray, S.; Thormann, U.; Kramer, I.; Sommer, U.; Budak, M.; Schumacher, M.; Bernhardt, A.; Lode, A.; Kern, C.; Rohnke, M.; et al. Mesoporous Bioactive Glass-Incorporated Injectable Strontium-Containing Calcium Phosphate Cement Enhanced Osteoconductivity in a Critical-Sized Metaphyseal Defect in Osteoporotic Rats. Bioengineering 2023, 10, 1203. https://doi.org/10.3390/bioengineering10101203
Ray S, Thormann U, Kramer I, Sommer U, Budak M, Schumacher M, Bernhardt A, Lode A, Kern C, Rohnke M, et al. Mesoporous Bioactive Glass-Incorporated Injectable Strontium-Containing Calcium Phosphate Cement Enhanced Osteoconductivity in a Critical-Sized Metaphyseal Defect in Osteoporotic Rats. Bioengineering. 2023; 10(10):1203. https://doi.org/10.3390/bioengineering10101203
Chicago/Turabian StyleRay, Seemun, Ulrich Thormann, Inga Kramer, Ursula Sommer, Matthäus Budak, Matthias Schumacher, Anne Bernhardt, Anja Lode, Christine Kern, Marcus Rohnke, and et al. 2023. "Mesoporous Bioactive Glass-Incorporated Injectable Strontium-Containing Calcium Phosphate Cement Enhanced Osteoconductivity in a Critical-Sized Metaphyseal Defect in Osteoporotic Rats" Bioengineering 10, no. 10: 1203. https://doi.org/10.3390/bioengineering10101203