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Article

Role of Dysregulated Ion Channels in Sensory Neurons in Chronic Kidney Disease-Associated Pruritus

1
Department of Urology, Jusendo General Hospital, Koriyama 9638585, Japan
2
Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Hirosaki 0368563, Japan
*
Author to whom correspondence should be addressed.
Medicines 2019, 6(4), 110; https://doi.org/10.3390/medicines6040110
Received: 12 September 2019 / Revised: 25 October 2019 / Accepted: 11 November 2019 / Published: 13 November 2019
(This article belongs to the Special Issue Pathogenesis and Treatment of Chronic Pruritus)
Background: We investigated ion channels at the skin, including peripheral nerve endings, which serve as output machines and molecular integrators of many pruritic inputs mainly received by multiple G protein-coupled receptors (GPCRs). Methods: Based on the level of chronic kidney disease–associated pruritus (CKD-aP), subjects were divided into two groups: non-CKD-aP (no or slight pruritus; n = 12) and CKD-aP (mild, moderate, or severe pruritus; n = 11). Skin samples were obtained from the forearm or elbow during operations on arteriovenous fistulas. We measured ion channels expressed at the skin, including peripheral nerve endings by RT-PCR: Nav1.8, Kv1.4, Cav2.2, Cav3.2, BKCa, Anoctamin1, TRPV1, TRPA1, and ASIC. Results: Expression of Cav3.2, BKCa, and anoctamin1 was significantly elevated in patients with CKD-aP. On the other hand, expression of TRPV1 was significantly reduced in these patients. We observed no significant difference in the levels of Cav2.2 or ASIC between subjects with and without CKD-aP. TRPA1, Nav1.8, and Kv1.4 were not expressed. Conclusions: It was concluded that this greater difference in the expression of ion channels in the skin tissue including, specially cutaneous peripheral nerve endings in CKD patients with CKD-aP may increase generator potential related to itching. View Full-Text
Keywords: uremic pruritus; ion channels; cell signaling; Cav3.2 calcium channel; RT-PCR; skin uremic pruritus; ion channels; cell signaling; Cav3.2 calcium channel; RT-PCR; skin
MDPI and ACS Style

Momose, A.; Yabe, M.; Chiba, S.; Kumakawa, K.; Shiraiwa, Y.; Mizukami, H. Role of Dysregulated Ion Channels in Sensory Neurons in Chronic Kidney Disease-Associated Pruritus. Medicines 2019, 6, 110. https://doi.org/10.3390/medicines6040110

AMA Style

Momose A, Yabe M, Chiba S, Kumakawa K, Shiraiwa Y, Mizukami H. Role of Dysregulated Ion Channels in Sensory Neurons in Chronic Kidney Disease-Associated Pruritus. Medicines. 2019; 6(4):110. https://doi.org/10.3390/medicines6040110

Chicago/Turabian Style

Momose, Akishi, Micihihiro Yabe, Shigetoshi Chiba, Kenjirou Kumakawa, Yasuo Shiraiwa, and Hiroki Mizukami. 2019. "Role of Dysregulated Ion Channels in Sensory Neurons in Chronic Kidney Disease-Associated Pruritus" Medicines 6, no. 4: 110. https://doi.org/10.3390/medicines6040110

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