Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals
2.2. Plant Material and Extraction of Essential Oil
2.3. GC-MS Analysis
2.4. Cell Culture
2.5. Measurement of Anti-Inflammatory Activity
2.6. Evaluation of Antioxidant Activity Using Cell-Based Assays
2.7. Evaluation of Antibacterial Activity
2.8. Cytotoxic Assays
3. Results and Discussion
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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RI DB-5 1 | RI S-Wax 2 | Identified Compounds | Relative CONCENTRATION (%) |
---|---|---|---|
903 | 991 | Tricyclene | 0.11 |
911 | 1010 | α-Thujene | 0.58 |
917 | 1006 | α-Pinene | 4.43 |
933 | 1035 | Camphene | 7.29 |
962 | 1085 | Sabinene | 2.02 |
965 | 1069 | β-Pinene | 2.51 |
982 | 1160 | dehydro-1,8-Cineole | 0.23 |
1011 | 1148 | α-Terpinene | 0.09 |
1019 | 1242 | ρ-Cymene | 1.20 |
1023 | 1172 | Limonene | 0.16 2 |
1025 | 1181 | 1,8-Cineole | 10.80 2 |
1053 | 1222 | γ-Terpinene | 0.29 |
1061 | 1443 | cis-Sabinene hydrate | 0.11 |
1082 | 1254 | Terpinolene | 0.09 |
1096 | 1405 | Filifolone | 0.15 |
1100 | 1531 | Linalol | 0.38 |
1102 | 1386 | α-Thujone | 0.08 |
1109 | 1405 | β-Thujone | 3.66 |
1117 | 1468 | Chrysanthenone | 3.76 |
1132 | 1614 | trans-Pinocarveol | 0.64 |
1136 | 1471 | Camphor | 30.48 |
1153 | 1522 | Pinocarvone | 0.27 |
1160 | 1664 | Borneol | 14.80 |
1171 | 1570 | Terpinen-4-ol | 0.81 |
1176 | 1601 | Thuj-3-en-10-al | 0.45 |
1185 | 1667 | α-Terpineol | 0.69 |
1187 | 1579 | Myrtenal | 0.11 |
1190 | 1752 | Myrtenol | 0.09 |
1216 | 1799 | trans-Carveol | 0.10 |
1257 | 1539 | cis-Chrysanthenyl acetate | 0.10 |
1282 | 1545 | Bornyl acetate | 5.53 |
1352 | 2113 | Eugenol | 0.16 |
1370 | 1648 | Isobornyl propionate | 0.75 |
1391 | 1880 | cis-Jasmone | 0.08 |
1411 | 1556 | β-Caryophyllene | 0.09 |
1446 | 1629 | α-Humulene | 0.21 |
1475 | 1667 | Germacrene D | 1.13 |
1482 | 1674 | β-Selinene | 0.12 |
1491 | 1690 | Bicyclogermacrene | 0.16 |
1496 | 1698 | α-Muurolene | ˂−Muuro |
1505 | 1701 | β-Bisabolene | 0.09 |
1499 | 1735 | Germacrene A | 0.09 |
1503 | 1721 | δ-Amorphene | 0.17 |
1509 | 1719 | γ-Cadinene (1513) | 0.15 |
1519 | 1742 | β-Sesquiphellandrene | 0.17 |
1522 | 1728 | δ-Cadinene | 0.63 |
1536 | 1865 | α-Calacorene | 0.19 |
1573 | 1922 | Caryophyllene oxide | 1.13 |
1593 | 1976 | Ledol | 0.65 |
1597 | 1970 | Rosifoliol | 0.07 |
1622 | 2015 | 1-epi-Cubenol | 0.05 |
1624 | 2124 | γ-Eudesmol | 0.11 |
1631 | 2138 | τ-Muurolol | 0.66 |
1635 | 2131 | τ-Cadinol | 0.19 |
1641 | 2171 | β-Eudesmol | ˂−Eudes |
1647 | 2178 | α-Cadinol | 0.05 |
1663 | 2044 | Valeranone | 0.07 |
1713 | 1880 | Pentadecanal | ˂entade |
Total | 99.23 |
Compounds | Anti-Inflammation IC50 (µg·mL−1) | Antioxidant IC50 (µg·mL−1) | Antibacterial IC50 (µg·mL−1) | Cytotoxicity IC50 (µg·mL−1) | |||
---|---|---|---|---|---|---|---|
E. coli | S. aureus | A549 | DLD1 | WS1 | |||
Essential oil | 72 ± 9 | 51 ± 11 | 241 ± 13 | 59 ± 5 | 232 ± 51 | 105 ± 18 | 292 ± 17 |
1,8-Cineole | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
α-Humulene | 15 ± 2 | >200 | >200 | >200 | 28 ± 1 | 43 ± 3 | 24 ± 3 |
α-Pinene | 30 ± 4 | 3.4 ± 0.2 | nd | nd | 35 ± 6 | 58 ±18 | 57 ± 4 |
α-Terpinene | 46 ± 4 | >200 | >200 | >200 | 63 ± 10 | 73 ± 7 | 53 ± 12 |
β-Caryophyllene | >200 | >200 | >200 | >200 | 55 ± 5 | 97 ± 6 | 59 ± 7 |
β-Pinene | 46 ± 9 | >200 | >200 | >200 | 43 ± 4 | 49.4 ± 0.3 | 29 ± 14 |
γ-Terpinene | >200 | >200 | >200 | 50 ± 9 | 112 ± 15 | >200 | 82 ± 15 |
p-Cymene | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
Borneol | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
Bornyl acetate | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
Camphene | >200 | >200 | >200 | >200 | 72 ± 2 | 75 ± 5 | 49 ± 1 |
Camphor | >200 | >200 | 22 ± 1 | 26 ± 3 | >200 | >200 | >200 |
Caryophyllene oxide | 183 ± 15 | 6.2 ± 0.5 | 97 ± 2 | 10.4 ± 0.9 | 36 ± 2 | 43 ± 3 | 50 ± 4 |
Limonene | >200 | >200 | >200 | >200 | 71 ± 6 | 31 ± 3 | 53 ± 12 |
Terpinen-4-ol | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
Terpinolene | >200 | >200 | >200 | >200 | >200 | >200 | >200 |
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Coté, H.; Boucher, M.-A.; Pichette, A.; Legault, J. Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents. Medicines 2017, 4, 34. https://doi.org/10.3390/medicines4020034
Coté H, Boucher M-A, Pichette A, Legault J. Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents. Medicines. 2017; 4(2):34. https://doi.org/10.3390/medicines4020034
Chicago/Turabian StyleCoté, Héloïse, Marie-Anne Boucher, André Pichette, and Jean Legault. 2017. "Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents" Medicines 4, no. 2: 34. https://doi.org/10.3390/medicines4020034