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Article

Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells

1
Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan
2
Laboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8578, Japan
3
Watarase Research Center, Kyorin Pharmaceutical Co., Ltd., 1848, Nogi, Nogi-machi, Shimotsuga-gun, Tochigi 329-0014, Japan
4
Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan
*
Author to whom correspondence should be addressed.
Toxics 2020, 8(4), 78; https://doi.org/10.3390/toxics8040078
Received: 12 August 2020 / Revised: 23 September 2020 / Accepted: 27 September 2020 / Published: 28 September 2020
(This article belongs to the Section Toxicology)
Although homeobox protein B13 (HOXB13) is an oncogenic transcription factor, its role in stress response has rarely been examined. We previously reported that knockdown of HOXB13 reduces the cytotoxicity caused by various oxidative stress inducers. Here, we studied the role of HOXB13 in cytotoxicity caused by hydrogen peroxide in human lung epithelial A549 cells. The knockdown of HOXB13 reduced hydrogen peroxide-induced cytotoxicity; however, this phenomenon was largely absent in the presence of antioxidants (Trolox or N-acetyl cysteine (NAC)). This suggests that HOXB13 may be involved in the cytotoxicity caused by hydrogen peroxide via the production of reactive oxygen species (ROS). Hydrogen peroxide also increased both the mRNA and protein levels of HOXB13. However, these increases were rarely observed in the presence of a transcriptional inhibitor, which suggests that hydrogen peroxide increases protein levels via increased transcription of HOXB13. Furthermore, cell death occurred in A549 cells that highly expressed HOXB13. However, this cell death was mostly inhibited by treatment with antioxidants. Taken together, our findings indicate that HOXB13 may be a novel factor involved in the induction of oxidative stress, which causes cell death via intracellular ROS production. View Full-Text
Keywords: HOXB13; hydrogen peroxide; oxidative stress; reactive oxygen species (ROS) HOXB13; hydrogen peroxide; oxidative stress; reactive oxygen species (ROS)
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MDPI and ACS Style

Endo, N.; Toyama, T.; Naganuma, A.; Saito, Y.; Hwang, G.-W. Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells. Toxics 2020, 8, 78. https://doi.org/10.3390/toxics8040078

AMA Style

Endo N, Toyama T, Naganuma A, Saito Y, Hwang G-W. Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells. Toxics. 2020; 8(4):78. https://doi.org/10.3390/toxics8040078

Chicago/Turabian Style

Endo, Naoki, Takashi Toyama, Akira Naganuma, Yoshiro Saito, and Gi-Wook Hwang. 2020. "Hydrogen Peroxide Causes Cell Death via Increased Transcription of HOXB13 in Human Lung Epithelial A549 Cells" Toxics 8, no. 4: 78. https://doi.org/10.3390/toxics8040078

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