Long-Term Exposure to Cadmium Causes Hepatic Iron Deficiency through the Suppression of Iron-Transport-Related Gene Expression in the Proximal Duodenum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Animal Treatments
2.3. Hepatotoxicity
2.4. Real-Time RT-PCR Analysis
2.5. Cd and Fe Concentrations
2.6. Serum UIBC and TIBC Quantification
2.7. Statistical Analysis
3. Results
3.1. Effect of Long-Term Exposure to Cd on Accumulation of Iron and Cd in the Liver
3.2. Changes in Body Weight and Hepatotoxicity in Mice Exposed to Cd for a Long Time
3.3. Effect of Long-Term Exposure to Cd on Iron Concentration, UIBC and TIBC in the Serum
3.4. Effect of Long-Term Exposure to Cd on the Expression of Genes Involved in Absorption of Heme Iron in the Proximal Duodenum
3.5. Effect of Long-Term Exposure to Cd on the Expression of Genes Involved in Absorption of Non-Heme Iron in the Proximal Duodenum
3.6. Effect of Long-Term Exposure to Cd on the Expression of Genes Involved in the Efflux of Iron from the Proximal Duodenum Enterocytes into Blood Vessels
3.7. Effect of Long-Term Exposure to Cd on Gene Expression of Hamp and Fpn1 in the Liver
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Names | Forward | Reverse | Product Size (bp) |
---|---|---|---|
Slc46a1 (HCP1) | ATCTACCCGGCCACTCTGAA | GGAACTCCGGGTGTGGATTA | 121 |
Hmox1 | ACCTTCCCGAACATCGACAG | GGAAGGCGGTCTTAGCCTCT | 121 |
Slc11a2 (Dmt1) | GGCTTTCTTATGAGCATTGCCTA | GGAGCACCCAGAGCAGCTTA | 97 |
Cybrd1 | GCAGCGGGCTCGAGTTTA | TTCCAGGTCCATGGCAGTCT | 103 |
Slc40a1 (Fpn1) | CTGTCGGCCAGATTATGACA | GAGCAGGGGTCTTCTGGTAA | 126 |
Heph | TTGTCTCATGAAGAACATTTACAGCAC | CATATGGCAATCAAAGCAGAAGA | 161 |
Hamp | GGCAGACATTGCGATACCAA | TGGCTCTAGGCTATGTTTTGCA | 128 |
Actin, beta (Actb) | CCTAAGGCCAACCGTGAAAA | AGGCATACAGGGACAGCACA | 100 |
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Tokumoto, M.; Lee, J.-Y.; Fujiwara, Y.; Satoh, M. Long-Term Exposure to Cadmium Causes Hepatic Iron Deficiency through the Suppression of Iron-Transport-Related Gene Expression in the Proximal Duodenum. Toxics 2023, 11, 641. https://doi.org/10.3390/toxics11070641
Tokumoto M, Lee J-Y, Fujiwara Y, Satoh M. Long-Term Exposure to Cadmium Causes Hepatic Iron Deficiency through the Suppression of Iron-Transport-Related Gene Expression in the Proximal Duodenum. Toxics. 2023; 11(7):641. https://doi.org/10.3390/toxics11070641
Chicago/Turabian StyleTokumoto, Maki, Jin-Yong Lee, Yasuyuki Fujiwara, and Masahiko Satoh. 2023. "Long-Term Exposure to Cadmium Causes Hepatic Iron Deficiency through the Suppression of Iron-Transport-Related Gene Expression in the Proximal Duodenum" Toxics 11, no. 7: 641. https://doi.org/10.3390/toxics11070641