Evaluation of In Vitro Genotoxicity of Polystyrene Nanoparticles in Human Peripheral Blood Mononuclear Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Particle Characterization and Preparation of NPs Suspensions
2.2. Biological Material
2.3. Lymphocyte Isolation
2.4. NPs Uptake Analysis
2.5. Analysis of Cell Viability and Primary DNA Damage (the Comet Assay)
2.6. The Micronucleus Assay
2.7. Statistical Analysis
3. Results
3.1. PS-NPs Characterization
3.2. Uptake Analysis
3.3. Cell Viability and Induction of Primary DNA Damage
3.4. The Micronucleus Assay
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Matrix | Time after Sonication, h | Highest Peak Size, nm | Mean Size, nm (SD) |
---|---|---|---|
(a) Distilled H2O | 0 | 71 | 80.4 (22.4) |
(b) RPMI 1640 | 0 | 79 | 114.9 (45.9) |
(c) RPMI 1640 | 3 | 105 | 202 (103.1) |
(d) RPMI 1640 | 24 | 17 | 47.5 (34.1) |
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Babonaitė, M.; Čepulis, M.; Kazlauskaitė, J.; Lazutka, J.R. Evaluation of In Vitro Genotoxicity of Polystyrene Nanoparticles in Human Peripheral Blood Mononuclear Cells. Toxics 2023, 11, 627. https://doi.org/10.3390/toxics11070627
Babonaitė M, Čepulis M, Kazlauskaitė J, Lazutka JR. Evaluation of In Vitro Genotoxicity of Polystyrene Nanoparticles in Human Peripheral Blood Mononuclear Cells. Toxics. 2023; 11(7):627. https://doi.org/10.3390/toxics11070627
Chicago/Turabian StyleBabonaitė, Milda, Matas Čepulis, Jūratė Kazlauskaitė, and Juozas Rimantas Lazutka. 2023. "Evaluation of In Vitro Genotoxicity of Polystyrene Nanoparticles in Human Peripheral Blood Mononuclear Cells" Toxics 11, no. 7: 627. https://doi.org/10.3390/toxics11070627