RNA-Seq Analysis of Testes from Mice Exposed to Neodymium Oxide
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Sample Collection
2.2. H&E Staining
2.3. Sperm Count and Morphology Assessment
2.4. RNA Library Construction and Sequencing
2.5. RNA Identification and Expression Analysis
2.6. ceRNA Networks Analysis
2.7. Protein–Protein Interaction (PPI) Network Construction
2.8. qPCR Confirmation
2.9. Statistical Analysis
3. Results
3.1. Effects of Nd2O3 Exposure on Testis and Sperm Count and Sperm Morphology
3.2. Expression Profiles of mRNA, miRNA, lncRNA, and circRNA
3.3. GO and KEGG Analysis of DEGs
3.4. ceRNA Network Construction
3.5. PPI Analysis of Differential mRNAs
3.6. Validation of Profiles via qRT-PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, S.; Bu, N.; Yun, Y.; Shi, X.; Wang, S.; Gao, Y. RNA-Seq Analysis of Testes from Mice Exposed to Neodymium Oxide. Toxics 2023, 11, 952. https://doi.org/10.3390/toxics11120952
Wang S, Bu N, Yun Y, Shi X, Wang S, Gao Y. RNA-Seq Analysis of Testes from Mice Exposed to Neodymium Oxide. Toxics. 2023; 11(12):952. https://doi.org/10.3390/toxics11120952
Chicago/Turabian StyleWang, Shurui, Ning Bu, Yudan Yun, Xuemin Shi, Suhua Wang, and Yanrong Gao. 2023. "RNA-Seq Analysis of Testes from Mice Exposed to Neodymium Oxide" Toxics 11, no. 12: 952. https://doi.org/10.3390/toxics11120952