Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cell Viability Assay
2.3. Transmission Electron Microscope
2.4. Lipid-ROS Assay by Flow Cytometry
2.5. Western Blot Analysis
2.6. Nitric Oxide (NO) Content Determination
2.7. Statistical Analysis
3. Results
3.1. PFOS Can Inhibit the Multiplication and Viability of HUVECs In Vitro
3.2. Ferroptosis of HUVECs Observed under Transmission Electron Microscope
3.3. PFOS Can Increase the Production of ROS
3.4. PFOS Can Induce Ferroptosis via Ferroptosis-Related Pathway
3.5. PFOS Can Decrease the Production of NO
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Cui, J.; Wang, P.; Yan, S.; Liang, Y.; Liu, D.; Ren, S. Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway. Toxics 2022, 10, 503. https://doi.org/10.3390/toxics10090503
Cui J, Wang P, Yan S, Liang Y, Liu D, Ren S. Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway. Toxics. 2022; 10(9):503. https://doi.org/10.3390/toxics10090503
Chicago/Turabian StyleCui, Jiajing, Pingwei Wang, Shuqi Yan, Yujun Liang, Dongge Liu, and Shuping Ren. 2022. "Perfluorooctane Sulfonate Induces Dysfunction of Human Umbilical Vein Endothelial Cells via Ferroptosis Pathway" Toxics 10, no. 9: 503. https://doi.org/10.3390/toxics10090503