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Toxics 2013, 1(1), 46-59; doi:10.3390/toxics1010046

Chronic Exposure to Particulate Nickel Induces Neoplastic Transformation in Human Lung Epithelial Cells

Sr. 1,2,3,*
Received: 16 October 2013 / Revised: 6 November 2013 / Accepted: 14 November 2013 / Published: 25 November 2013
(This article belongs to the Special Issue Feature Papers)
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Nickel is a well-known human lung carcinogen with the particulate form being the most potent; however, the carcinogenic mechanism remains largely unknown. Few studies have investigated the genotoxicity and carcinogenicity of nickel in its target cell, human bronchial epithelial cells. Thus, the goal of this study was to investigate the effects of particulate nickel in human lung epithelial cells. We found that nickel subsulfide induced concentration- and time-dependent increases in both cytotoxicity and genotoxicity in human lung epithelial cells (BEP2D). Chronic exposure to nickel subsulfide readily induced cellular transformation, inducing 2.55, 2.9 and 2.35 foci per dish after exposure to 1, 2.5 and 5 μg/cm2 nickel subsulfide, respectively. Sixty-one, 100 and 70 percent of the foci isolated from 1, 2.5, and 5 μg/cm2 nickel subsulfide treatments formed colonies in soft agar and the degree of soft agar colony growth increased in a concentration-dependent manner. Thus, chronic exposure to particulate nickel induces genotoxicity and cellular transformation in human lung epithelial cells.
Keywords: particulate nickel; nickel subsulfide; chromosome damage; transformation particulate nickel; nickel subsulfide; chromosome damage; transformation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Holmes, A.L.; The, T.; Thompson, K.; Mason, M.; Kandpal, S.; Zheng, T.; Wise, J.P., Sr. Chronic Exposure to Particulate Nickel Induces Neoplastic Transformation in Human Lung Epithelial Cells. Toxics 2013, 1, 46-59.

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